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  本文選題：肺損傷　切入點：腸　出處：《細胞與分子免疫學(xué)雜志》2014年04期 　論文類型：期刊論文

【摘要】：目的通過建立大鼠腸缺血再灌注肺損傷(IIRI)模型,觀察急性肺損傷(ALI)時大鼠肺組織中具有CCCH鋅指結(jié)構(gòu)域的蛋白12D(Zc3h12d)表達水平的變化。方法健康成年的雄性SD大鼠40只,隨機分成對照組(contro1)、缺血再灌注30 min組(IR30 min)、缺血再灌注60 min組(IR60 min)和缺血再灌注120 min組(IR120 min),每組10只。對照組(contro1)行假手術(shù),4個缺血再灌注組缺血60 min后分別再灌注30、60、120 min,在相應(yīng)時間點處死大鼠,獲取血清及肺組織。術(shù)后各樣本行肺濕干質(zhì)量比(W/D)檢測、HE染色觀察肺組織病變,ELISA檢測血清及肺勻漿中TNF-α及IL-1β含量,實時定量PCR(qRT-PCR)檢測肺組織Zc3h12d mRNA的表達,免疫組化、Western blot法檢測肺組織Zc3h12d蛋白的表達。結(jié)果與對照組比較,再灌注損傷組病理切片可見肺泡壁增寬,肺泡腔內(nèi)出血,肺濕干質(zhì)量比值(W/D)顯著增大(P0.05),qRT-PCR、免疫組化和Western blot法顯示再灌注損傷組肺組織Zc3h12d的mRNA和蛋白表達水平在再灌注120 min時明顯降低(P0.05)。結(jié)論小腸缺血再灌注肺損傷時肺組織中Zc3h12d基因表達下調(diào),提示在肺損傷時Zc3h12d蛋白可能起保護作用。
[Abstract]:Objective to observe the expression of CCCH zinc finger domain protein 12DX Zc3h12d in the lung tissue of rats with acute lung injury (Ali) by establishing the model of intestinal ischemia-reperfusion lung injury (IIRI). Methods 40 adult male SD rats were enrolled in this study. The rats were randomly divided into control group (control group), ischemia reperfusion group (30 min group), ischemia reperfusion group (60 min group) and ischemia reperfusion group (120 min group) with 10 rats in each group. Sham operation was performed in the control group (n = 10), and reperfusion was performed in 4 ischemia reperfusion groups after ischemia for 60 min. The rats were killed at the corresponding time point. Serum and lung tissue were obtained. After operation, lung wet / dry mass ratio (WPR / DX) was used to detect the content of TNF- 偽 and IL-1 尾 in serum and lung homogenate by Elisa, and the expression of Zc3h12d mRNA in lung tissue was detected by real-time quantitative PCRQ RT-PCR. Results compared with the control group, the alveolar wall was enlarged and the alveolar cavity hemorrhage was observed in the reperfusion injury group. Lung wet / dry weight ratio (W / D) significantly increased P0.05 / qRT-PCR.Immunohistochemical and Western blot methods showed that the expression of Zc3h12d mRNA and protein in lung tissue of reperfusion group decreased significantly at 120 min reperfusion. Conclusion the lung tissue of small intestine ischemia-reperfusion lung injury is significantly decreased by immunohistochemistry and Western blot method. Conclusion\\\; The expression of Zc3h12d gene was down-regulated. These results suggest that Zc3h12d protein may play a protective role in lung injury.
【作者單位】： 第四軍醫(yī)大學(xué)附屬唐都醫(yī)院胸腔外科;
【基金】：國家自然科學(xué)基金(30470873)
【分類號】：R363

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本文編號：1597654