本文選題：氣管　切入點(diǎn)：組織工程　出處：《第四軍醫(yī)大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:氣管疾病由于其解剖生理特點(diǎn),直接危機(jī)患者的生命。外科治療是解決氣管疾病的首選方法,但對(duì)于切除范圍長(zhǎng)度在6cm以上的病變,直接端端吻合成功機(jī)率很小。因此,尋求一種理想的氣管替代物是解決這一問題的關(guān)鍵。利用人工材料、自體組織和氣管移植等方法重建氣管,術(shù)后都存在著一定程度的狹窄,嚴(yán)重影響患者的生活質(zhì)量。近年來(lái)組織工程學(xué)的興起及其突飛猛進(jìn)的發(fā)展,可望在這一領(lǐng)域做出突破性的貢獻(xiàn)。依據(jù)我們長(zhǎng)期以來(lái)在氣管重建領(lǐng)域的研究,對(duì)氣管重建后軟骨細(xì)胞的再生,氣管粘膜上皮的培養(yǎng)及氣管重建后的再血管化等方面,已經(jīng)積累了大量的實(shí)驗(yàn)基礎(chǔ)。本實(shí)驗(yàn)以骨髓間質(zhì)干細(xì)胞(mesenchymal stem cells,MSCs)為種子細(xì)胞,利用骨形態(tài)發(fā)生蛋白-2(bone morphogenetic protein,BMP-2)誘導(dǎo)MSCs定向分化為軟骨細(xì)胞,復(fù)合聚乙醇酸(poly glycolic acid,PGA)支架材料上塑形,形成軟骨組織。通過(guò)本實(shí)驗(yàn)的進(jìn)一步研究,為構(gòu)建組織工程氣管軟骨及氣管粘膜上皮的復(fù)合提供理論和實(shí)驗(yàn)基礎(chǔ),最終構(gòu)建組織工程氣管,從結(jié)構(gòu)和功能上完整重建氣管,克服氣管重建后軟骨吸收造成的軟化性狹窄,以及缺乏上皮覆蓋導(dǎo)致的瘢痕性狹窄。 方法:從兔骨髓中分離出MSCs,進(jìn)行體外培養(yǎng)增殖。在培養(yǎng)基中加入BMP-2,誘導(dǎo)MSCs向軟骨細(xì)胞分化,觀察其形態(tài)學(xué)改變及堿性磷酸酶(alkaline phosphatase,ALP)活性,并進(jìn)行ALP免疫組化染色觀察。以不同濃度BMP-2
[Abstract]:Objective: because of its anatomical and physiological characteristics, trachea diseases directly threaten the life of patients. Surgical treatment is the first choice to solve trachea diseases, but the success rate of direct end-to-end anastomosis is very small for lesions with a resection range of more than 6 cm. To find an ideal tracheal substitute is the key to solve this problem. Using artificial materials, autologous tissue and trachea transplantation, there is a certain degree of stenosis after operation. The rise of tissue engineering and its rapid development in recent years are expected to make a breakthrough contribution in this field, according to our long-term research in the field of trachea reconstruction. A large number of experimental bases have been accumulated for the regeneration of chondrocytes after trachea reconstruction, the culture of tracheal mucosa epithelium and the vascularization after trachea reconstruction. In this experiment, mesenchymal stem cells (MSCs) were used as seed cells. Bone morphogenetic protein (BMP) -2bone morphogenetic protein (BMP-2) was used to induce MSCs to differentiate into chondrocytes and to form cartilage tissue on the scaffold material of poly glycolic acidine. It provides a theoretical and experimental basis for the construction of tracheal cartilage and epithelium in tissue engineering. Finally, the trachea is constructed. The trachea can be reconstructed completely in terms of structure and function, and the softening stenosis caused by cartilage absorption after trachea reconstruction can be overcome. And scarring stenosis due to a lack of epithelial coverage. Methods: MSCs were isolated from rabbit bone marrow and cultured in vitro. BMP-2 was added to the culture medium to induce the differentiation of MSCs into chondrocytes, and the morphological changes and alkaline phosphatase (ALP) activity were observed. The immunohistochemical staining of ALP was carried out. BMP-2 with different concentrations was observed.
【學(xué)位授予單位】：第四軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R329.2

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