目的:研究蛋白酶體抑制劑MG132對大鼠腸缺血再灌注腸、肺損傷的保護(hù)作用與調(diào)控Nrf2/Keap1-ARE通路之間的關(guān)系。方法:將32只健康雄性SD大鼠隨機(jī)分為4組:正常對照組、I/R組、對照+MG132預(yù)處理組、I/R+MG132預(yù)處理組。I/R組及I/R+MG132組使用無創(chuàng)動(dòng)脈夾夾閉腸系膜上動(dòng)脈（SMA）1小時(shí)、再灌注2小時(shí),建立大鼠腸缺血再灌注損傷模型。MG132預(yù)處理組于缺血前30分鐘腹腔注射0.5 mg/kg MG132,正常對照組僅進(jìn)行剖腹術(shù)及SMA分離術(shù)。再灌注結(jié)束后,腹主動(dòng)脈取血并留取肺腸組織樣本。光鏡下觀察大鼠腸、肺石蠟切片組織形態(tài)學(xué)改變,評價(jià)組織損傷程度,檢測血清中IL-6、TNF-α水平、26S蛋白酶體活性及組織勻漿中超氧化物歧化酶（SOD）、髓過氧化物酶（MPO）活性,采用免疫組織化學(xué)法和免疫印跡法檢測肺、腸組織中核轉(zhuǎn)錄因子Nrf2（NF-E2-related factor 2）和血紅素氧合酶-1（Heme Oxygenase-1,HO-1）蛋白表達(dá)。結(jié)果:1.與正常對照組相比,I/R組:（1）肺、腸組織有明顯的水腫、滲出和炎性細(xì)胞浸潤（P<0.01,P... 

【文章來源】：大連醫(yī)科大學(xué)遼寧省

【文章頁數(shù)】：46 頁

【學(xué)位級別】：碩士

【文章目錄】：
中文摘要
英文摘要
前言
材料和方法
結(jié)果
討論
結(jié)論
參考文獻(xiàn)
綜述
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附錄
致謝


【參考文獻(xiàn)】：
期刊論文
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