【摘要】：目的建立HO-8910卵巢癌熒光原位移植瘤模型,并進(jìn)行分期研究。方法將人卵巢癌HO-8910細(xì)胞株在細(xì)胞培養(yǎng)瓶中培養(yǎng)至10代,選熒光穩(wěn)定表達(dá)的細(xì)胞注射到裸鼠皮下,待成瘤后通過顯微外科手術(shù)將體積為1mm3腫瘤塊植入到實(shí)驗(yàn)裸鼠卵巢黏膜上,在活體成像技術(shù)下觀察腫瘤的生長(zhǎng)和轉(zhuǎn)移情況,并應(yīng)用病理學(xué)檢測(cè)方法對(duì)腫瘤組織及附近組織進(jìn)行觀察。結(jié)果活體成像技術(shù)成功追蹤了腫瘤的發(fā)展與轉(zhuǎn)移,病理結(jié)果顯示腫瘤細(xì)胞的轉(zhuǎn)移趨勢(shì),2者與中醫(yī)臨床卵巢癌分期相結(jié)合,確立了卵巢癌分期。結(jié)論成功建立卵巢癌原位移植瘤模型,再現(xiàn)了腫瘤臨床演變過程。其3期分期為腫瘤研究及抗腫瘤藥物療效判定研究提供了實(shí)驗(yàn)基礎(chǔ)。
[Abstract]:Objective to establish a fluorescent orthopaedic transplantation model of HO-8910 ovarian cancer and study its staging. Methods Human ovarian cancer cell line HO-8910 was cultured in cell culture bottle for 10 generations. The cells stably expressed by fluorescence were injected into the skin of nude mice. After the tumor was formed, the volume of 1mm3 tumor was implanted into the ovarian mucosa of experimental nude mice by microsurgery. The growth and metastasis of the tumor were observed by in vivo imaging technique, and the tumor tissue and nearby tissues were observed by pathological examination. Results the development and metastasis of tumor were successfully followed by in vivo imaging. The pathological results showed the metastasis trend of tumor cells. The two were combined with the clinical stage of ovarian cancer in traditional Chinese medicine to establish the stage of ovarian cancer. Conclusion the orthotopic transplantation model of ovarian cancer was successfully established and the clinical evolution of the tumor was reproduced. The stage 3 stage provides an experimental basis for tumor research and antineoplastic drug efficacy evaluation.
【作者單位】： 南京中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院;南陽理工學(xué)院張仲景國(guó)醫(yī)國(guó)藥學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(81373990);國(guó)家自然科學(xué)基金青年基金(81503267;81503270) 江蘇省高校自然科學(xué)基金(15KJB360003)
【分類號(hào)】：R-332;R737.31

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1 王磊,陳衛(wèi)昌,陳桂林,馬文雄,謝學(xué)順;人結(jié)腸癌裸鼠原位移植瘤模型的建立[J];蘇州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2005年02期

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