【摘要】：目的研究Smad蛋白抑制劑SIS3對體外細(xì)粒棘球蚴原頭節(jié)的影響。方法建立體外細(xì)粒棘球蚴培養(yǎng)體系,以不同濃度(12.5、25、50和100μmol/L)、不同作用時間(24、48、72和96h)對原頭節(jié)進(jìn)行藥物(Smad蛋白抑制劑SIS3)干預(yù)試驗,伊紅染色和掃描及透射電鏡觀察不同濃度藥物干預(yù)過程中原頭節(jié)活力及蟲體超微結(jié)構(gòu)的變化。結(jié)果SIS3干預(yù)48h,50和100μmol/L SIS3組與空白對照組原頭節(jié)活力顯著下降;作用96h,50和100μmol/L SIS3組原頭節(jié)活力分別下降(10.06±2.79)%和(19.49±2.63)%。隨著藥物濃度的增加,掃描電鏡觀察原頭節(jié)體表絨毛排列紊亂,吸盤結(jié)構(gòu)變形,頂突小鉤脫落;透射電鏡觀察藥物組原頭節(jié)表層微毛減少或消失,合胞體帶變薄,胞漿空泡化,蟲體內(nèi)部出現(xiàn)脂滴等。結(jié)論 Smad信號通路抑制劑SIS3對體外細(xì)粒棘球蚴有抑制作用,為治療細(xì)粒棘球蚴病提供了理論基礎(chǔ)。
[Abstract]:Objective to study the effect of Smad protein inhibitor SIS3 on the procephalic ganglia of Echinococcus granulosus in vitro. Methods the culture system of Echinococcus granulosus in vitro was established. Different concentrations of Echinococcus granulosus were treated with different concentrations (12.5, 25, 50 and 100 渭 mol / L), for 24, 48, 72 and 96 hours). The drug (Smad protein inhibitor SIS3) was used to interfere with Echinococcus granulosus. The changes of the activity of procephalic ganglia and the ultrastructure of parasite during different concentrations of drug intervention were observed by eosin staining, scanning electron microscope and transmission electron microscope. Results after SIS3 intervention for 48 h, the activity of primary head node in 50 渭 mol / L SIS3 group and blank control group decreased significantly, and that in 50 渭 mol / L SIS3 group and 100 渭 mol / L SIS3 group decreased by (10.06 鹵2.79)% and (19.49 鹵2.63)% at 96 h, respectively. With the increase of drug concentration, the arrangement of villi on the surface of the original head node was disordered, the structure of the sucker was deformed and the hook of the apical process fell off with the increase of the concentration of the drug. Transmission electron microscope showed that in the drug group, the microhair on the surface of the original head decreased or disappeared, the syncytial zone became thinner, the cytoplasm was vacuolated, and lipid droplets appeared in the insect body. Conclusion SIS3, an inhibitor of Smad signaling pathway, has inhibitory effect on Echinococcus granulosus in vitro, which provides a theoretical basis for the treatment of Echinococcus granulosus.
【作者單位】： 新疆醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院;新疆醫(yī)科大學(xué)第一附屬醫(yī)院/臨床醫(yī)學(xué)研究院 新疆包蟲病基礎(chǔ)醫(yī)學(xué)重點實驗室;
【基金】：新疆維吾爾自治區(qū)自然科學(xué)基金項目(No.2015211C029) 新疆醫(yī)科大學(xué)大學(xué)生創(chuàng)新性實驗計劃項目基金項目(No.國家級201510760019) 新疆重大疾病醫(yī)學(xué)重點實驗室開放課題項目(No.SKLIB-XJMDR-2016-3)
【分類號】：R383.33
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本文編號：2479609