發(fā)布時間：2019-05-17 13:48

【摘要】：目的探討未知功能蛋白C7orf42對體外培養(yǎng)的大鼠肝細(xì)胞BRL-3A增殖的影響。方法基因干涉下調(diào)C7orf42表達(dá)后,用MTT、Ed U摻入法檢測C7orf42對BRL-3A細(xì)胞增殖的影響,流式細(xì)胞術(shù)檢測細(xì)胞周期進(jìn)程以及Reat-time PCR和Western blotting檢測細(xì)胞增殖相關(guān)基因表達(dá)。結(jié)果 MTT法檢測表明,轉(zhuǎn)染C7orf42干涉片段后48h,干涉組比陰性對照組細(xì)胞活力降低11%(P0.05);Ed U摻入法檢測表明,實驗組的Ed U陽性細(xì)胞比率比對照組降低了13%(P0.05);流式細(xì)胞術(shù)檢測表明,干涉組比陰性對照組S+G2/M期的細(xì)胞數(shù)降低12%(P0.05);Reat-time PCR檢測表明,干涉C7orf42表達(dá)后細(xì)胞增殖相關(guān)基因JUN、CCND1、MYC和CCNA2的表達(dá)分別下調(diào)26%、31%、37%和14%(P0.05);Western blotting檢測表明,干涉C7orf42表達(dá)后細(xì)胞增殖相關(guān)蛋白JUN、CCND1、MYC和CCNA2的表達(dá)分別下調(diào)59%、54%、18%和27%(P0.05)。結(jié)論 C7orf42可能通過上調(diào)細(xì)胞增殖相關(guān)蛋白JUN、CCND1、MYC和CCNA2的表達(dá),促進(jìn)體外培養(yǎng)的大鼠肝細(xì)胞BRL-3A增殖。
[Abstract]:Objective to investigate the effect of unknown functional protein C7orf42 on the proliferation of rat hepatocytes BRL-3A in vitro. Methods after down-regulating the expression of C7orf42 by gene interference, the effect of C7orf42 on the proliferation of BRL-3A cells was detected by MTT,Ed U incorporation, the cell cycle process was detected by flow cytometry, and the expression of genes related to cell proliferation was detected by Reat-time PCR and Western blotting. Results MTT assay showed that 48 hours after C7orf42 interference, the cell vitality of the interference group was 11% lower than that of the negative control group (P 0.05). The results of Ed U incorporation showed that the percentage of Ed U positive cells in the experimental group was 13% lower than that in the control group (P 0.05), and the number of cells in S G 2 鈮，

本文編號：2479140