【摘要】：背景:理論上,從肝纖維化大鼠骨髓中提取經(jīng)過(guò)體內(nèi)病理環(huán)境"篩選"作用后的內(nèi)皮祖細(xì)胞,應(yīng)更能適應(yīng)肝纖維化微環(huán)境,移植入纖維化肝臟后應(yīng)更傾向于分化為成熟內(nèi)皮細(xì)胞,參與肝內(nèi)血管重建。目的:探討來(lái)源于大鼠肝纖維化體內(nèi)環(huán)境的骨髓源性內(nèi)皮祖細(xì)胞移植對(duì)大鼠肝纖維化的治療作用。方法:將28只Wistar大鼠隨機(jī)分為3組:正常組(n=8)皮下注射橄欖油,2次/周;模型組(n=10)皮下注射四氯化碳3 m L/kg,2次/周,首劑加倍,分別于第2,3,5周末尾靜脈輸注生理鹽水;實(shí)驗(yàn)組(n=10)皮下注射四氯化碳3 m L/kg,2次/周,首劑加倍,分別于第2,3,5周末尾靜脈輸注肝纖維化大鼠骨髓源性內(nèi)皮祖細(xì)胞懸液。注射6周末,進(jìn)行肝功能、凝血功能、肝組織相關(guān)蛋白及肝組織病理檢查。結(jié)果與結(jié)論:(1)肝組織病理:模型組肝組織呈廣泛而嚴(yán)重的肝細(xì)胞脂肪變性和壞死,門靜脈和中央靜脈周圍炎癥細(xì)胞浸潤(rùn),匯管區(qū)擴(kuò)大及大量纖維組織增生;與模型組相比,實(shí)驗(yàn)組肝細(xì)胞變性壞死、炎癥細(xì)胞浸潤(rùn)及纖維組織增生等均有所減輕;(2)肝組織相關(guān)蛋白:與正常組相比,模型組透明質(zhì)酸、層粘連蛋白和Ⅲ型前膠原、血管內(nèi)皮生長(zhǎng)因子、表皮生長(zhǎng)因子水平升高(P0.05);與模型組相比,實(shí)驗(yàn)組透明質(zhì)酸、層粘連蛋白和Ⅲ型前膠原水平明顯下降(P0.05),血管內(nèi)皮生長(zhǎng)因子、表皮生長(zhǎng)因子水平升高(P0.05);(3)肝功能、凝血功能:與正常組相比,模型組肝功能、凝血功能嚴(yán)重受損(P0.05);與模型組相比,實(shí)驗(yàn)組肝功能明顯改善(P0.05);(4)結(jié)果表明:來(lái)源于肝纖維化體內(nèi)環(huán)境的骨髓源性內(nèi)皮祖細(xì)胞移植能有效治療大鼠肝纖維化,其作用機(jī)制可能是通過(guò)促進(jìn)肝臟血管新生來(lái)實(shí)現(xiàn)的。
[Abstract]:Background: theoretically, endothelial progenitor cells (EPCs) extracted from bone marrow of rats with hepatic fibrosis should be more adaptable to the microenvironment of hepatic fibrosis after being "screened" by pathological environment in vivo. After transplantation into fibrotic liver, it is more likely to differentiate into mature endothelial cells and participate in intrahepatic vascular remodeling. Aim: to investigate the therapeutic effect of bone marrow derived endothelial progenitor cell transplantation from rat liver fibrosis environment on rat liver fibrosis. Methods: 28 Wistar rats were randomly divided into 3 groups: normal group (n = 8) was subcutaneously injected with olive oil twice a week; In the model group (n 鈮，

本文編號(hào)：2471603