【摘要】：目的：SW2是一種新型特異性的間質(zhì)磁共振淋巴造影劑,經(jīng)趾蹼皮下注射后可清晰顯影兔雙下肢淋巴管和胭窩淋巴結(jié)�，F(xiàn)探討SW2經(jīng)舌粘膜下注射對(duì)頸淋巴顯影的應(yīng)用價(jià)值,為了解頭頸部淋巴引流規(guī)律及診斷頭頸部惡性腫瘤的轉(zhuǎn)移情況提供新的方法。 方法：采用3.0 GE核磁共振檢查。選取純種新西蘭大白兔12只,在每只大白兔雙側(cè)舌緣粘膜下各注射0.2mL SW2,按摩注射部位30S。分別于造影劑注射之前及注射后10、15、20、25、30、35、40、50、90min及4h進(jìn)行三維增強(qiáng)磁共振淋巴造影成像。測(cè)量不同延遲時(shí)間引流區(qū)頸部淋巴結(jié)的信號(hào)強(qiáng)度,計(jì)算不同時(shí)間信號(hào)強(qiáng)化率(E%),并繪制時(shí)間-信號(hào)強(qiáng)化率曲線。采用SPSS11.5軟件包對(duì)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)分析。分別于實(shí)驗(yàn)前和實(shí)驗(yàn)四周后對(duì)大白兔進(jìn)行血常規(guī)和血生化檢查,并進(jìn)行肝臟和腎臟的病理學(xué)檢查。 結(jié)果：SW2能夠特異性顯影兔頸部淋巴結(jié)及一、二級(jí)淋巴管,顯像清晰,血管無(wú)顯影。頸部淋巴結(jié)在注射造影劑后約30min信號(hào)強(qiáng)度達(dá)到峰值,E%為344%,在注射后20-50min可獲得最佳強(qiáng)化效果。在注射后4h淋巴結(jié)及淋巴管造影劑基本擴(kuò)清,信號(hào)強(qiáng)度恢復(fù)到正常水平。頸部淋巴結(jié)在不同時(shí)間的信號(hào)強(qiáng)化率進(jìn)行統(tǒng)計(jì)學(xué)分析,差別有統(tǒng)計(jì)學(xué)意義(P0.05)。注射顯影劑前后血常規(guī)及生化常規(guī)檢查均無(wú)顯著差異,肝臟、腎臟病理學(xué)檢查均未見(jiàn)明顯異常。各組實(shí)驗(yàn)動(dòng)物在皮下穿刺及注射造影劑后1w內(nèi)均未明顯不良反應(yīng)。 結(jié)論：SW2作為IMRLG,能快速有效的顯示頸部引流區(qū)淋巴管的走形以及頸部淋巴結(jié)形態(tài),顯影清晰,顯影持續(xù)時(shí)間長(zhǎng),對(duì)淋巴組織顯影有特異性,是一種優(yōu)異的具有潛在臨床及科研價(jià)值的淋巴造影劑。
[Abstract]:Objective: SW2 is a new kind of specific interstitial magnetic resonance lymphatic contrast agent, which can be used to develop the lymphatic and fossa lymph nodes of the two lower limbs of the rabbit after the subcutaneous injection of the webbed webbed. The application value of SW2 transmucosal injection on the development of cervical lymph is discussed, and a new method is provided to understand the regularity of head and neck lymphatic drainage and to diagnose the metastasis of head and neck malignant tumor. Methods: 3.0 GE nuclear magnetic resonance (MRI) was used. 12 pure-bred New Zealand white rabbits were selected, and each rabbit was injected with 0. 2mL of SW2 under the submucous membrane of the double-side tongue of each white rabbit, and the massage injection site 30S and three-dimensional enhanced magnetic resonance lymphography was performed before and after the injection of the contrast agent, 10, 15, 20, 25, 30, 35, 40, 50, 90min, and 4h after the injection of the contrast agent, respectively.. Measure the signal strength of the neck lymph nodes in the drainage area of different delay time, calculate the signal strength of different time (E%), and draw the time-signal strength curve. Statistical analysis of data using the SPSS11.5 software package. Blood routine and blood biochemical examination were performed on the white rabbits before and after the experiment, and the pathological examination of the liver and the kidney was performed. Results: SW2 was able to specifically develop the lymph nodes of the neck of the rabbit and the lymph nodes of the first and the second stage. The imaging was clear and the blood vessels were not significant. The signal intensity of the cervical lymph node after injection of the contrast agent reached the peak value, and the E% was 344%. The best enhancement effect was obtained after 20-50min after injection. Fruit. After injection, the lymph node and lymphatic contrast medium were substantially expanded and the signal intensity recovered to normal water. The signal enhancement rate of the cervical lymph node at different time was statistically analyzed, and the difference was statistically significant (P0.05).). There was no significant difference in blood routine and biochemical routine examination before and after injection of the developer. No significant difference was found in liver and kidney pathology. The experimental animals in each group were not significantly negative in 1w after the subcutaneous puncture and the injection of the contrast agent. Conclusion: SW2, as the IMRLG, can quickly and effectively display the shape of the lymph vessels in the neck drainage area and the shape of the neck lymph nodes, the development is clear, the development duration is long, the development of the lymph tissue is specific, it is an excellent lymphatic system with potential clinical and scientific research value.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R445.2;R-332

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