【摘要】：目的研究PI3K-Akt-mTOR信號通路對Foxp3基因表達的影響。方法將SPF級昆明系小鼠60只隨機分為對照組(A)和實驗組(B、C、D),B、C、D三組分別灌胃雷帕霉素0.2、0.4、0.6 mg.d-1,A組每天予以無菌水灌胃,共3周。3周后,無菌條件下心臟采血,EDTA抗凝,分離脾臟,制備單細胞懸液,采用流式細胞儀檢測小鼠外周血和脾臟中CD4+CD25+調(diào)節(jié)性T細胞水平(CD4+CD25+Treg細胞占CD4+T細胞的百分比),RT-PCR檢測小鼠脾細胞Foxp3mRNA的表達,免疫組織化學(xué)SP法染色觀察小鼠脾臟中p-mTOR蛋白表達的變化,并利用計算機圖像分析技術(shù)測量各實驗組及對照組中p-mTOR蛋白表達的平均光密度。結(jié)果 Foxp3 mRNA在實驗組(B、C、D)小鼠脾臟中表達的程度分別為(0.4853±0.0574、0.7886±0.1085、0.9639±0.2015),明顯高于對照組A(0.1345±0.0271)(P0.05);實驗組(B、C、D)小鼠脾臟中p-mTOR蛋白表達的平均光密度分別為(0.2326±0.0431、0.1156±0.0223、0.0556±0.0041),明顯低于對照組A(0.4223±0.0534)(P0.05);兩指標經(jīng)pearson相關(guān)分析,小鼠脾臟中Foxp3mRNA的表達與p-mTOR蛋白表達呈負相關(guān)。結(jié)論抑制PI3K-Akt-mTOR信號通路會促使Foxp3的表達增強;PI3K-Akt-mTOR信號通路的激活程度與Foxp3的表達程度呈負相關(guān)。
[Abstract]:Objective to study the effect of PI3K-Akt-mTOR signaling pathway on Foxp3 gene expression. Methods 60 Kunming mice of SPF grade were randomly divided into two groups: control group (A) and experimental group (group D). After 3 weeks, blood was collected from the heart, EDTA was anticoagulant, spleen was isolated and single cell suspension was prepared under aseptic condition. The levels of CD4 CD25 regulatory T cells (the percentage of CD4 CD25 Treg cells in CD4 T cells) in peripheral blood and spleen of mice were detected by flow cytometry, and the expression of Foxp3mRNA in spleen cells of mice was detected by RT-PCR. Immunohistochemical SP staining was used to observe the expression of p-mTOR protein in the spleen of mice, and the average optical density of p-mTOR protein expression in each experimental group and control group was measured by computer image analysis. Results the expression of Foxp3 mRNA in the spleen of the experimental group was (0.4853 鹵0.0574) 0.7886 鹵0.1085 鹵0.9639 鹵0.2015, which was significantly higher than that of the control group A (0.1345 鹵0.0271) (P0.05). The average optical density of p-mTOR protein expression in the spleen of the experimental group was (0.2326 鹵0.0431) 0.1156 鹵0.022 3n 0.0556 鹵0.0041, which was significantly lower than that of the control group A (0.4223 鹵0.0534) (P0.05). By pearson correlation analysis, the expression of Foxp3mRNA in mouse spleen was negatively correlated with the expression of p-mTOR protein. Conclusion inhibition of PI3K-Akt-mTOR signaling pathway can increase the expression of Foxp3, and the activation of PI3K-Akt-mTOR signaling pathway is negatively correlated with the expression of Foxp3.
【作者單位】： 安徽醫(yī)科大學(xué)第一附屬醫(yī)院心臟血管外科;
【分類號】：R-332

【參考文獻】

中國期刊全文數(shù)據(jù)庫 前1條

1 楊明珍;吳德沛;岑建農(nóng);袁燕慧;�；輼s;朱子玲;馮宇鋒;;雷帕霉素誘導(dǎo)Balb/c小鼠CD4~+ CD25~+ foxp3~+調(diào)節(jié)性T細胞增殖[J];中國藥理學(xué)通報;2007年06期

【共引文獻】

中國期刊全文數(shù)據(jù)庫 前2條

1 王婷玉;李俊;;CD4~+CD25~+調(diào)節(jié)性T細胞在類風(fēng)濕性關(guān)節(jié)炎中的研究進展[J];中國藥理學(xué)通報;2008年11期

2 秦瑾;陳海英;叢斌;魏平;張敬各;李巧霞;馬春玲;李淑瑾;付麗紅;于峰;;外源性LTB4對CIA小鼠Treg/Th17脾細胞分化的作用[J];中國藥理學(xué)通報;2009年09期

中國碩士學(xué)位論文全文數(shù)據(jù)庫 前1條

1 沈筱蕓;雷帕霉素促進小鼠CD4~+CD25~+T細胞增殖和Foxp3表達的機制研究[D];第二軍醫(yī)大學(xué);2010年

【二級參考文獻】

中國期刊全文數(shù)據(jù)庫 前2條

1 陸莉,林志彬;雷帕霉素對小鼠脾淋巴細胞增殖及產(chǎn)生細胞因子的影響[J];中國藥理學(xué)通報;2001年03期

2 羅璨,郭蓮軍;�；撬釋Υ笫蠹毙阅X缺血神經(jīng)元凋亡的影響[J];中國藥理學(xué)通報;2005年09期

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