重組人白介素-10對大鼠肺缺血再灌注損傷的保護作用
[Abstract]:Aim: to investigate the protective effect of recombinant human interleukin-10 (rhIL-10) on lung ischemia-reperfusion injury in rats and its possible mechanism. Methods: single lung ischemia-reperfusion model was established with left pulmonary arteriovenous occlusion and left main bronchus occlusion under microscope. 72 Wistar rats were randomly divided into control group (Control group), ischemia-reperfusion group (IR group) and rhIL-10 drug intervention group (rhIL-10 group). Each group was divided into ischemia 45 min, reperfusion 60120min 3 time points. Each time point 8, altogether 24. The plasma TNF- 偽 and malondialdehyde (MDA) (MDA) levels were measured and the pathological changes of lung tissue were observed. Results: there was no significant difference in MDA in ischemic 45min group (P0.05). IR group increased significantly during reperfusion 60min and 120min, while rhIL-10 group was higher than Control group, but significantly lower than IR group (P0.05). Compared with Control group, the level of TNF- 偽 in IR group was significantly higher than that in IR group (P0.05), but there was no significant change in rhIL-10 group (P0.05). The level of TNF- 偽 in IR group was significantly higher than that in Control group (P0.05), while that in rhIL-10 group was significantly higher than that in Control group (P0.05), but significantly lower than that in IR group (P0.05). Pulmonary pathology indicated that the alveolar septum and alveolar inflammatory cells infiltrated, a large number of inflammatory cells in alveolar cavity exudated, bleeding, and with the prolongation of reperfusion time, the injury was aggravated. The pathological injury in rhIL-10 group was lighter than that in IR group. Conclusion: rhIL-10 may protect lung ischemia-reperfusion injury by inhibiting oxygen free radical formation, neutrophil infiltration and TNF- 偽 reaction.
【作者單位】: 山西醫(yī)科大學(xué)附屬第二醫(yī)院胸外科;
【分類號】:R363
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