【摘要】：目的:探討重組人白介素-10(rhIL-10)對(duì)大鼠肺缺血再灌注損傷的保護(hù)作用及可能機(jī)制。方法:顯微鏡下夾閉左肺動(dòng)靜脈和左主支氣管建立單肺缺血再灌注模型。72只Wistar大鼠隨機(jī)分為對(duì)照組(Control組)、缺血再灌注組(IR組)、rhIL-10藥物干預(yù)組(rhIL-10組),每組分為缺血45min、再灌注60、120min 3個(gè)時(shí)間點(diǎn)。每個(gè)時(shí)間點(diǎn)8只,共24只。按上述時(shí)間點(diǎn)處死收集標(biāo)本,檢測(cè)血漿TNF-α、丙二醛(MDA)水平,觀(guān)察肺組織病理變化。結(jié)果:各組MDA在缺血45min時(shí)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),再灌注60min和120min時(shí)IR組明顯升高,而rhIL-10組雖較Control組升高,但明顯低于IR組(P0.05)。與Control組比較,IR組缺血45min時(shí)TNF-α水平明顯升高(P0.05),rhIL-10組無(wú)明顯變化(P0.05)。再灌注60min和120min時(shí)IR組TNF-α水平較Control組明顯升高(P0.05),而rhIL-10組雖較Control組顯著升高(P0.05),但明顯低于IR組(P0.05)。肺病理提示肺泡間隔及肺泡內(nèi)炎性細(xì)胞浸潤(rùn),肺泡腔內(nèi)大量炎性細(xì)胞滲出、出血,且隨著再灌注時(shí)間的延長(zhǎng),損傷加重。rhIL-10組病理?yè)p傷均較IR組為輕。結(jié)論:rhIL-10可能通過(guò)抑制氧自由基生成、中性粒細(xì)胞浸潤(rùn)、TNF-α反應(yīng)對(duì)肺缺血再灌注損傷起保護(hù)作用。
[Abstract]:Aim: to investigate the protective effect of recombinant human interleukin-10 (rhIL-10) on lung ischemia-reperfusion injury in rats and its possible mechanism. Methods: single lung ischemia-reperfusion model was established with left pulmonary arteriovenous occlusion and left main bronchus occlusion under microscope. 72 Wistar rats were randomly divided into control group (Control group), ischemia-reperfusion group (IR group) and rhIL-10 drug intervention group (rhIL-10 group). Each group was divided into ischemia 45 min, reperfusion 60120min 3 time points. Each time point 8, altogether 24. The plasma TNF- 偽 and malondialdehyde (MDA) (MDA) levels were measured and the pathological changes of lung tissue were observed. Results: there was no significant difference in MDA in ischemic 45min group (P0.05). IR group increased significantly during reperfusion 60min and 120min, while rhIL-10 group was higher than Control group, but significantly lower than IR group (P0.05). Compared with Control group, the level of TNF- 偽 in IR group was significantly higher than that in IR group (P0.05), but there was no significant change in rhIL-10 group (P0.05). The level of TNF- 偽 in IR group was significantly higher than that in Control group (P0.05), while that in rhIL-10 group was significantly higher than that in Control group (P0.05), but significantly lower than that in IR group (P0.05). Pulmonary pathology indicated that the alveolar septum and alveolar inflammatory cells infiltrated, a large number of inflammatory cells in alveolar cavity exudated, bleeding, and with the prolongation of reperfusion time, the injury was aggravated. The pathological injury in rhIL-10 group was lighter than that in IR group. Conclusion: rhIL-10 may protect lung ischemia-reperfusion injury by inhibiting oxygen free radical formation, neutrophil infiltration and TNF- 偽 reaction.
【作者單位】： 山西醫(yī)科大學(xué)附屬第二醫(yī)院胸外科;
【分類(lèi)號(hào)】：R363

【共引文獻(xiàn)】

相關(guān)期刊論文 前1條

1 孫慶;鄧存良;;白介素-10與肝星狀細(xì)胞的激活[J];瀘州醫(yī)學(xué)院學(xué)報(bào);2007年03期

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 盧延旭,路景濤,陳敏珠;白細(xì)胞介素-10對(duì)大鼠佐劑性關(guān)節(jié)炎的治療作用[J];中國(guó)藥理學(xué)通報(bào);2000年01期

2 張喜平;大黃素的藥理作用研究概況[J];中國(guó)藥理學(xué)通報(bào);2003年08期

3 洪新,郭振輝,毛寶齡,錢(qián)桂生;地塞米松和IL-10對(duì)hPBMC促炎細(xì)胞因子產(chǎn)生和核轉(zhuǎn)錄因子活化的影響[J];中國(guó)病理生理雜志;2002年11期

4 馬東來(lái);李俊;陳敏珠;徐叔云;;卡芬尼對(duì)大鼠佐劑性關(guān)節(jié)炎治療作用的免疫機(jī)制[J];中國(guó)免疫學(xué)雜志;1992年02期

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