青海地區(qū)藏族男性飲酒行為與乙醇代謝酶基因ADH3和ALDH2多態(tài)型分布研究
發(fā)布時間：2018-12-18 08:26
【摘要】：目的探討青海地區(qū)藏族男性飲酒行為模式及乙醛脫氫酶2(ALDH2)與乙醇脫氫酶3(ADH3)基因多態(tài)型分布。 方法(1)現(xiàn)場流行病學(xué)調(diào)查用于收集研究對象的飲酒行為。在青海省疾病預(yù)防控制中心及青海省藏醫(yī)院的體檢人群中,提前找出在當(dāng)天體檢的所有藏族男性,然后每隔一個體檢號進行抽樣。在青海大學(xué)醫(yī)學(xué)院藏醫(yī)系,以年級分層后采用整群抽樣的方法抽取各班同學(xué),被抽取班級的全部男同學(xué)進行調(diào)查。排除不愿意參與調(diào)查的人群。漢族抽取方法如上。所有被抽取的調(diào)查者均表示愿意參與該項目方可調(diào)查。三個采樣點共抽取758人,剔除資料不完整者26份,實際分析資料640份,調(diào)查對象年齡為39.7±14.6。其中藏族430人,漢族302人,均為男性。(2)采用血液基因組DNA提取系統(tǒng)試劑盒提取DNA,聚合酶鏈式反應(yīng)-限制性片段長度多態(tài)型(PCR-RFLP)方法對乙醇脫氫酶3(ADH3)和乙醛脫氫酶2(ALDH2)基因多態(tài)型進行檢測。 結(jié)果(1)藏、漢族的整體飲酒率分別為66.47%和69.54%；藏、漢族人群中安全飲酒者分別占39.07%和41.39%,危險飲酒者分別占27.67%和28.16%；藏、漢族均以低度白酒為主分別占67.94%和64.28%；藏、漢族飲酒者中飲酒方式以眾飲佐菜為主分別占79.44%和58.09%,其次為眾飲不佐菜分別占12.89%和19.05%,差異有統(tǒng)計學(xué)意義(p0.01)；藏、漢族飲酒人群中認為飲酒是一種交友方式的分別57.49%和51.43%,其次是認為飲酒是一種享受的分別占27.87%和19.05%,差異有統(tǒng)計學(xué)意義(p0.01)；飲酒者臉紅是發(fā)展成為危險飲酒者的保護因素,吸煙和身邊人飲酒是危險飲酒者的危險因素。(2)藏、漢族ADH3*2等位基因頻率分別占0.08和0.14,ALDH2*2等位基因的分別占0.22和0.19,ADH3*2和ALDH2*2等位基因在藏、漢族分布差異有統(tǒng)計學(xué)意義(p50.01)；藏族男性中組合基因型分布以野生純合性ADH3和ALDH2占優(yōu)勢為50%,其次是正常性ADH3和缺陷型ALDH2基因組合型、缺陷型ADH3和正常性ALDH2和缺陷性ADH3和ALDH2基因組合型分別占35.11%、10.46%和4.41%。(3)藏族飲酒行為與ADH3、ALDH2基因相關(guān),與ALDH2基因的更為密切。 結(jié)論藏族男性的飲酒率高,飲酒者年輕化,飲酒人群中仍存在不安全飲酒行為。藏族飲酒行為與ADH3、ALDH2基因具有相關(guān)性,與ALDH2基因的相關(guān)性更為密切。與漢族人群相比,藏族人群中ADH3*1和ALDH2*2占優(yōu)勢,表明藏族飲酒者酒后不良反應(yīng)較漢族飲酒者多。藏族男性中以功能正常性ADH3和ALDH2基因組合型占優(yōu)勢,該類人群較其他基因組合型飲酒者更易發(fā)展成為酗酒者。同時有35.11%的人以正常型ADH3和缺陷型ALDH2基因組合型為主,該人群如果繼續(xù)飲酒則對機體的損害將更為嚴重。健康工作者更加需要注意那些處于危險邊緣的危險飲酒者和有害飲酒者,如果該危險飲酒者和有害飲酒者還沒有發(fā)展到酒依賴的程度,能夠早期及時的采取干預(yù),能夠有效的減少酒量和酒精所致的問題。
[Abstract]:Objective to investigate the pattern of drinking behavior and the distribution of acetaldehyde dehydrogenase 2 (ALDH2) and alcohol dehydrogenase 3 (ADH3) gene polymorphism in Tibetan men in Qinghai. Methods (1) the field epidemiological investigation was used to collect the drinking behavior of the subjects. In the physical examination population of the Center for Disease Control and Prevention of Qinghai Province and the Tibetan Hospital of Qinghai Province, all Tibetan men who were examined on the same day were identified in advance, and then sampled every other physical examination number. In the Department of Tibetan Medicine, College of Medicine, Qinghai University, every class was sampled by cluster sampling after grade stratification, and all the male students in the class were investigated. Exclude people who are unwilling to participate in the investigation. The Han nationality extraction method is as above. All respondents indicated that they were willing to participate in the project before the survey. A total of 758 people were sampled from three sampling sites, 26 samples were excluded from incomplete data, 640 actual data were analyzed, and the age of the subjects was 39.7 鹵14.6. Of them, 430 were Tibetan and 302 were Han, all of them were male. (2) DNA, extraction kit was used to extract DNA,. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of ethanol dehydrogenase 3 (ADH3) and aldehyde dehydrogenase 2 (ALDH2) gene. Results (1) in Tibet, the overall drinking rate of Han nationality was 66.47% and 69.54 respectively, the safe drinking rate was 39.07% and 41.39%, the dangerous drinking rate was 27.67% and 28.1616%, respectively. 67.94% and 64.28% of the Han nationality were mainly low-alcohol liquor. Among the drinkers of the Han nationality, 79.44% and 58.09% of the drinkers were mainly mixed with vegetables, followed by 12.89% and 19.05, respectively. The difference was statistically significant (p0.01). Among the Han people, 57.49% and 51.43% considered drinking as a way of making friends, followed by 27.87% and 19.05% of them thought that drinking was a kind of enjoyment, the difference was statistically significant (p0.01). Blushing was the protective factor to develop into a dangerous drinker. Smoking and drinking were risk factors. (2) the frequency of ADH3*2 allele in Han nationality was 0.08 and 0.14, respectively. The percentage of ALDH2*2 alleles was 0.22 and 0.19, respectively, and the distribution of ADH3t2 and ALDH2*2 alleles was significantly different in Han nationality (p50.01). In Tibetan males, wild homozygous ADH3 and ALDH2 were dominant, followed by normal ADH3 and defective ALDH2 gene combinations, and defective ADH3 and normal ALDH2 and defective ADH3 and ALDH2 gene combinations accounted for 35.11%, respectively. 10.46% and 4.41% respectively. (3) Tibetan drinking behavior is related to ADH3,ALDH2 gene and is more closely related to ALDH2 gene. Conclusion the drinking rate of Tibetan males is high, the drinkers are younger, and unsafe drinking behavior still exists in the drinkers. Tibetan drinking behavior is correlated with ADH3,ALDH2 gene, and more closely with ALDH2 gene. Compared with the Han nationality, the ADH3*1 and ALDH2*2 were dominant in the Tibetan population, which indicated that the adverse effects of drinking were more in the Tibetan drinkers than in the Han drinkers. The functional normal ADH3 and ALDH2 gene combinations were dominant in Tibetan males, which were more likely to develop into alcoholics than those with other gene combinations. At the same time, 35.11% of the population were mainly composed of normal ADH3 and defective ALDH2 gene combination, and the damage to the body would be more serious if the population continued to drink. Health workers need to pay more attention to dangerous drinkers and harmful drinkers who are on the verge of danger and who are able to intervene early and in time if they have not developed a degree of alcohol dependence. Can effectively reduce alcohol consumption and alcohol caused by the problem.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R394
本文編號：2385628
[Abstract]:Objective to investigate the pattern of drinking behavior and the distribution of acetaldehyde dehydrogenase 2 (ALDH2) and alcohol dehydrogenase 3 (ADH3) gene polymorphism in Tibetan men in Qinghai. Methods (1) the field epidemiological investigation was used to collect the drinking behavior of the subjects. In the physical examination population of the Center for Disease Control and Prevention of Qinghai Province and the Tibetan Hospital of Qinghai Province, all Tibetan men who were examined on the same day were identified in advance, and then sampled every other physical examination number. In the Department of Tibetan Medicine, College of Medicine, Qinghai University, every class was sampled by cluster sampling after grade stratification, and all the male students in the class were investigated. Exclude people who are unwilling to participate in the investigation. The Han nationality extraction method is as above. All respondents indicated that they were willing to participate in the project before the survey. A total of 758 people were sampled from three sampling sites, 26 samples were excluded from incomplete data, 640 actual data were analyzed, and the age of the subjects was 39.7 鹵14.6. Of them, 430 were Tibetan and 302 were Han, all of them were male. (2) DNA, extraction kit was used to extract DNA,. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of ethanol dehydrogenase 3 (ADH3) and aldehyde dehydrogenase 2 (ALDH2) gene. Results (1) in Tibet, the overall drinking rate of Han nationality was 66.47% and 69.54 respectively, the safe drinking rate was 39.07% and 41.39%, the dangerous drinking rate was 27.67% and 28.1616%, respectively. 67.94% and 64.28% of the Han nationality were mainly low-alcohol liquor. Among the drinkers of the Han nationality, 79.44% and 58.09% of the drinkers were mainly mixed with vegetables, followed by 12.89% and 19.05, respectively. The difference was statistically significant (p0.01). Among the Han people, 57.49% and 51.43% considered drinking as a way of making friends, followed by 27.87% and 19.05% of them thought that drinking was a kind of enjoyment, the difference was statistically significant (p0.01). Blushing was the protective factor to develop into a dangerous drinker. Smoking and drinking were risk factors. (2) the frequency of ADH3*2 allele in Han nationality was 0.08 and 0.14, respectively. The percentage of ALDH2*2 alleles was 0.22 and 0.19, respectively, and the distribution of ADH3t2 and ALDH2*2 alleles was significantly different in Han nationality (p50.01). In Tibetan males, wild homozygous ADH3 and ALDH2 were dominant, followed by normal ADH3 and defective ALDH2 gene combinations, and defective ADH3 and normal ALDH2 and defective ADH3 and ALDH2 gene combinations accounted for 35.11%, respectively. 10.46% and 4.41% respectively. (3) Tibetan drinking behavior is related to ADH3,ALDH2 gene and is more closely related to ALDH2 gene. Conclusion the drinking rate of Tibetan males is high, the drinkers are younger, and unsafe drinking behavior still exists in the drinkers. Tibetan drinking behavior is correlated with ADH3,ALDH2 gene, and more closely with ALDH2 gene. Compared with the Han nationality, the ADH3*1 and ALDH2*2 were dominant in the Tibetan population, which indicated that the adverse effects of drinking were more in the Tibetan drinkers than in the Han drinkers. The functional normal ADH3 and ALDH2 gene combinations were dominant in Tibetan males, which were more likely to develop into alcoholics than those with other gene combinations. At the same time, 35.11% of the population were mainly composed of normal ADH3 and defective ALDH2 gene combination, and the damage to the body would be more serious if the population continued to drink. Health workers need to pay more attention to dangerous drinkers and harmful drinkers who are on the verge of danger and who are able to intervene early and in time if they have not developed a degree of alcohol dependence. Can effectively reduce alcohol consumption and alcohol caused by the problem.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R394
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