青海地區(qū)藏族男性飲酒行為與乙醇代謝酶基因ADH3和ALDH2多態(tài)型分布研究
[Abstract]:Objective to investigate the pattern of drinking behavior and the distribution of acetaldehyde dehydrogenase 2 (ALDH2) and alcohol dehydrogenase 3 (ADH3) gene polymorphism in Tibetan men in Qinghai. Methods (1) the field epidemiological investigation was used to collect the drinking behavior of the subjects. In the physical examination population of the Center for Disease Control and Prevention of Qinghai Province and the Tibetan Hospital of Qinghai Province, all Tibetan men who were examined on the same day were identified in advance, and then sampled every other physical examination number. In the Department of Tibetan Medicine, College of Medicine, Qinghai University, every class was sampled by cluster sampling after grade stratification, and all the male students in the class were investigated. Exclude people who are unwilling to participate in the investigation. The Han nationality extraction method is as above. All respondents indicated that they were willing to participate in the project before the survey. A total of 758 people were sampled from three sampling sites, 26 samples were excluded from incomplete data, 640 actual data were analyzed, and the age of the subjects was 39.7 鹵14.6. Of them, 430 were Tibetan and 302 were Han, all of them were male. (2) DNA, extraction kit was used to extract DNA,. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of ethanol dehydrogenase 3 (ADH3) and aldehyde dehydrogenase 2 (ALDH2) gene. Results (1) in Tibet, the overall drinking rate of Han nationality was 66.47% and 69.54 respectively, the safe drinking rate was 39.07% and 41.39%, the dangerous drinking rate was 27.67% and 28.1616%, respectively. 67.94% and 64.28% of the Han nationality were mainly low-alcohol liquor. Among the drinkers of the Han nationality, 79.44% and 58.09% of the drinkers were mainly mixed with vegetables, followed by 12.89% and 19.05, respectively. The difference was statistically significant (p0.01). Among the Han people, 57.49% and 51.43% considered drinking as a way of making friends, followed by 27.87% and 19.05% of them thought that drinking was a kind of enjoyment, the difference was statistically significant (p0.01). Blushing was the protective factor to develop into a dangerous drinker. Smoking and drinking were risk factors. (2) the frequency of ADH3*2 allele in Han nationality was 0.08 and 0.14, respectively. The percentage of ALDH2*2 alleles was 0.22 and 0.19, respectively, and the distribution of ADH3t2 and ALDH2*2 alleles was significantly different in Han nationality (p50.01). In Tibetan males, wild homozygous ADH3 and ALDH2 were dominant, followed by normal ADH3 and defective ALDH2 gene combinations, and defective ADH3 and normal ALDH2 and defective ADH3 and ALDH2 gene combinations accounted for 35.11%, respectively. 10.46% and 4.41% respectively. (3) Tibetan drinking behavior is related to ADH3,ALDH2 gene and is more closely related to ALDH2 gene. Conclusion the drinking rate of Tibetan males is high, the drinkers are younger, and unsafe drinking behavior still exists in the drinkers. Tibetan drinking behavior is correlated with ADH3,ALDH2 gene, and more closely with ALDH2 gene. Compared with the Han nationality, the ADH3*1 and ALDH2*2 were dominant in the Tibetan population, which indicated that the adverse effects of drinking were more in the Tibetan drinkers than in the Han drinkers. The functional normal ADH3 and ALDH2 gene combinations were dominant in Tibetan males, which were more likely to develop into alcoholics than those with other gene combinations. At the same time, 35.11% of the population were mainly composed of normal ADH3 and defective ALDH2 gene combination, and the damage to the body would be more serious if the population continued to drink. Health workers need to pay more attention to dangerous drinkers and harmful drinkers who are on the verge of danger and who are able to intervene early and in time if they have not developed a degree of alcohol dependence. Can effectively reduce alcohol consumption and alcohol caused by the problem.
【學(xué)位授予單位】:蘭州大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2012
【分類號】:R394
【參考文獻】
相關(guān)期刊論文 前10條
1 吳建中,丁建華,高長明,李蘇平,臧宇,周建農(nóng),蘇平,劉燕婷,周學(xué)富,王如鴻,丁保國;江蘇漢族人乙醛脫氫酶2基因型分布及其與飲酒習(xí)慣的關(guān)系[J];癌變.畸變.突變;2002年02期
2 黃代新,楊慶恩,趙貴森;片段長度差異等位基因特異性PCR—一種改良的SNP分型新方法[J];法醫(yī)學(xué)雜志;2005年01期
3 李東;;淺析酗酒的危害及治療——以社會工作為視角[J];法制與社會;2009年15期
4 鄭流波;潘仰中;蔡運昌;柳桂娥;張玉瓊;;貴州省社區(qū)人群飲酒行為及影響因素流行病學(xué)調(diào)查[J];貴州醫(yī)藥;2009年07期
5 鄧源;于紅;李秀敏;豐玉蓉;;乙醇對胃黏膜的損傷作用[J];華北國防醫(yī)藥;2006年05期
6 李艷君;;日本人與酒[J];黑龍江科技信息;2010年22期
7 楊靜;李南方;李濤;曹梅;邵亮;馬永華;;乙醛脫氫酶基因G1951A多態(tài)性與男性飲酒行為的關(guān)系[J];科學(xué)技術(shù)與工程;2007年21期
8 曹西蓉,吳德生;AS-PCR在ADH2、ALDH2基因多態(tài)型分析中的應(yīng)用[J];環(huán)境與職業(yè)醫(yī)學(xué);2004年05期
9 郭坤亮,季克良,王昌祿;酒精代謝及其相關(guān)基因遺傳多態(tài)性[J];釀酒科技;2005年07期
10 文守海;趙生元;;互助青稞酒風(fēng)格成因探討[J];釀酒科技;2011年09期
,本文編號:2385628
本文鏈接:http://sikaile.net/xiyixuelunwen/2385628.html