【摘要】：目的:觀察蟲草多糖反復(fù)腦缺血再灌注模型小鼠在蟲草多糖干預(yù)下對學(xué)習(xí)記憶及腦組織超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(methane dicarboxylic aldehyde,MDA)的影響。方法:建立反復(fù)腦缺血再灌注小鼠模型,采用跳臺法和避暗法對小鼠學(xué)習(xí)記憶能力進行檢測,并測定其腦組織SOD活性及MDA含量。結(jié)果:與空白組比較,模型組小鼠測試期觸電潛伏期顯著縮短(P0.01)、訓(xùn)練期及測試期錯誤反應(yīng)次數(shù)均顯著增加(P0.01),說明造模成功。與模型組比較,尼莫地平能夠顯著延長小鼠觸電潛伏期、顯著降低小鼠錯誤反應(yīng)次數(shù)(P0.01);大劑量、中劑量蟲草多糖能夠顯著延長小鼠觸電潛伏期(P0.01),顯著降低訓(xùn)練期及測試期小鼠跳臺錯誤反應(yīng)次數(shù)(P0.01),小劑量蟲草多糖能夠明顯降低訓(xùn)練期小鼠跳臺錯誤反應(yīng)次數(shù)(P0.05)。與空白組比較,模型組能夠顯著降低潛伏期(P0.01)、增加錯誤反應(yīng)次數(shù)(P0.01),說明造模成功。與模型組比較,尼莫地平能夠顯著延長小鼠潛伏期(P0.01)、減少小鼠錯誤反應(yīng)次數(shù)(P0.01),大劑量、中劑量、小劑量蟲草多糖能夠明顯增加小鼠潛伏期(P0.01,P0.05),大劑量、中劑量、小劑量蟲草多糖能顯著降低小鼠錯誤反應(yīng)次數(shù)(P0.01)。與空白組比較,模型組小鼠腦組織中的SOD活性顯著降低(P0.01),MDA含量明顯升高(P0.05),說明造模成功。與模型組比較,蟲草多糖各劑量組均能明顯升高腦缺血小鼠腦組織的SOD活性(P0.05,P0.01),降低MDA含量(P0.05),且與劑量呈正相關(guān)性。結(jié)論:蟲草多糖對腦缺血再灌注損傷模型小鼠的學(xué)習(xí)記憶能力有一定改善作用,能夠提高腦內(nèi)SOD活性、降低MDA含量,增強自由基的清除能力,具有較好的應(yīng)用前景。
[Abstract]:Aim: to observe the effects of Cordyceps polysaccharides on learning and memory, superoxide dismutase (superoxide dismutase,SOD) and malondialdehyde (methane dicarboxylic aldehyde,MDA) in cerebral ischemia reperfusion model mice. Methods: the mice model of repeated cerebral ischemia-reperfusion was established. The learning and memory abilities of mice were measured by bench jumping method and dark avoidance method. The activity of SOD and the content of MDA in brain tissue were measured. Results: compared with the blank group, the electric shock latency in the model group was significantly shortened (P0.01), and the number of false reactions during the training period and the test period were significantly increased (P0.01), which indicated that the model was successful. Compared with the model group, nimodipine could significantly prolong the latency of electric shock in mice and decrease the number of wrong reactions in mice (P0.01). Cordyceps polysaccharides in high dose and middle dose could significantly prolong the latency of electric shock (P0.01), and significantly reduce the number of false reaction (P0.01) during the training period and the test period. Low dose Cordyceps polysaccharides could significantly reduce the number of false reaction in mice during training period (P0.05). Compared with the blank group, the model group could significantly reduce the latency (P0.01) and increase the number of errors (P0.01), which indicated that the model was successful. Compared with the model group, nimodipine significantly prolonged the latency of mice (P0.01), decreased the number of errors in mice (P0.01), and significantly increased the latency of mice (P0.01) in large, medium and low dose Cordyceps polysaccharides. P05), large dose, middle dose, low dose Cordyceps polysaccharide can significantly reduce the number of mouse error reaction (P0.01). Compared with the blank group, the activity of SOD in the brain tissue of the model group was significantly decreased (P0.01), MDA content was significantly increased (P0.05), indicating that the model was successful. Compared with the model group, Cordyceps polysaccharides could significantly increase the activity of SOD (P0.05, P0.01) and decrease the content of MDA (P0.05) in the brain tissue of mice with cerebral ischemia, and had a positive correlation with the dose of Cordyceps polysaccharide. Conclusion: Cordyceps polysaccharides can improve the learning and memory ability of mice with cerebral ischemia-reperfusion injury, increase the activity of SOD, decrease the content of MDA and enhance the scavenging ability of free radicals.
【作者單位】： 鄭州澍青醫(yī)學(xué)高等�？茖W(xué)校;
【基金】：鄭州市科技創(chuàng)新團隊資助項目(121PCXTD520)
【分類號】：R285.5;R-332

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