【摘要】：目的檢測并比較Rv1789刺激結(jié)核分枝桿菌(Mtb)感染人群和健康對照者外周血IFN-γ水平。方法以原核表達(dá)體系構(gòu)建、表達(dá)、純化rRv1789。從南京市疾病預(yù)防控制中心選擇39位受試者,參考臨床結(jié)核病診斷標(biāo)準(zhǔn)和rCM-WBIA診斷標(biāo)準(zhǔn)將其分為TB感染組(包括結(jié)核病患者和潛伏感染者)和健康對照組,進(jìn)行rRv1789-WBIA試驗。結(jié)果成功構(gòu)建pET30b-Rv1789質(zhì)粒,SDS-PAGE鑒定表達(dá)產(chǎn)物分子質(zhì)量單位為44ku,與預(yù)期相符;Western blot證實該蛋白能被相應(yīng)抗體識別。rRv1789刺激Mtb感染組產(chǎn)生的IFN-γ水平顯著高于健康對照組(P0.01);rCM和rRv1789刺激受檢者全血IFN-γ水平具有顯著相關(guān)性(r=0.616,P0.01)。結(jié)論 PE/PPE家族蛋白Rv1789具有抗原性,可用作Mtb感染快速診斷和結(jié)核疫苗候選抗原。
[Abstract]:Objective to detect and compare the levels of IFN- 緯 in peripheral blood of Rv1789 stimulated Mycobacterium tuberculosis (Mtb) infected population and healthy controls. Methods rRv1789. was constructed, expressed and purified by prokaryotic expression system. Thirty nine subjects were selected from Nanjing Centers for Disease Control and Prevention. They were divided into TB infection group (including tuberculosis patients and latent infections) and healthy control group according to the diagnostic criteria of clinical tuberculosis and rCM-WBIA. The rRv1789-WBIA test was carried out. Results the pET30b-Rv1789 plasmid was successfully constructed, and the molecular weight unit of the expressed product was 44ku. which was consistent with the expectation. Western blot confirmed that the protein could be recognized by the corresponding antibody. The level of IFN- 緯 in Mtb infected group stimulated by rRv1789 was significantly higher than that in healthy control group (P0.01). There was a significant correlation between rCM and rRv1789 stimulated whole blood IFN- 緯 levels (r = 0.616, P 0.01). Conclusion PE/PPE family protein Rv1789 has antigenicity and can be used for rapid diagnosis of Mtb infection and candidate antigen for tuberculosis vaccine.
【作者單位】： 南京市婦幼保健院;安徽理工大學(xué)醫(yī)學(xué)院病原生物學(xué)教研室;皖南醫(yī)學(xué)院;
【基金】：安徽省高校自然科學(xué)研究重點項目(No.KJ2016A211) 國家級大學(xué)生創(chuàng)新創(chuàng)業(yè)計劃訓(xùn)練項目(No.201510361093、No.201510361096) 安徽理工大學(xué)博士研究基金項目(No.11565)
【分類號】：R378.911
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本文編號：2339570