【摘要】：目的:目前模擬臨床膿毒癥最佳的動物模型為嚙齒類動物的盲腸結(jié)扎穿孔(CLP)模型,其主要方法是分別通過控制盲腸穿刺針粗細(xì)、穿孔個(gè)數(shù)和盲腸結(jié)扎的長度來控制實(shí)驗(yàn)動物的死亡率,但在實(shí)際應(yīng)用過程中其實(shí)驗(yàn)結(jié)果可重復(fù)性較差。本研究旨在建立以標(biāo)測盲腸體積為基礎(chǔ)的CLP模型來進(jìn)一步控制動物感染嚴(yán)重程度與死亡率,同時(shí)模擬以往文獻(xiàn)中以標(biāo)測盲腸長度為基礎(chǔ)的CLP模型進(jìn)行對比,探索提高模型穩(wěn)定性的可行性方案。 方法:采用雄性SD大鼠建立盲腸結(jié)扎穿孔模型,分長度標(biāo)測結(jié)扎及體積標(biāo)測結(jié)扎兩大組,長度標(biāo)測組用絲線測量由回盲瓣至盲腸頂端全長,按不同長度比例分亞組結(jié)扎盲腸(盲腸全長的50%及75%),體積標(biāo)測組用亞甲藍(lán)標(biāo)記盲腸體積,按不同體積分亞組結(jié)扎盲腸(0.5ml,1.0ml, 1.5ml, 2.0ml和2.5ml);結(jié)扎后均用21號針頭單次貫通穿孔盲腸,術(shù)后6小時(shí)進(jìn)行血培養(yǎng)及腹腔分泌物涂片培養(yǎng)并觀察7天死亡率。 結(jié)果:長度標(biāo)測結(jié)扎組內(nèi)50%和75%組7天死亡率分別為90%和100%,兩組間無顯著差異(n=10, p =0.661)。體積標(biāo)測結(jié)扎組內(nèi)死亡率隨盲腸結(jié)扎體積增加而上升,0.5ml,1.0ml,1.5ml,2.0ml和2.5ml各組7天死亡率分別為40%、50%、60%、80%和100%,各組間存在顯著差異(n=10, p =0.002),而假手術(shù)組全部存活。長度標(biāo)測結(jié)扎組50%和75%亞組平均存活時(shí)間分別為1.621±0.605天和1.150±0.099天。體積標(biāo)測結(jié)扎組平均存活時(shí)間從0.5ml結(jié)扎亞組的4.708±0.893天到2.5ml結(jié)扎亞組的1.175±0.240天。CLP術(shù)后6小時(shí)血培養(yǎng)及腹腔分泌物涂片培養(yǎng)除假手術(shù)組外均為陽性。 結(jié)論:手術(shù)操作過程及實(shí)驗(yàn)數(shù)據(jù)顯示盲腸體積結(jié)扎穿孔法是一項(xiàng)更容易進(jìn)行的標(biāo)準(zhǔn)化操作,是實(shí)驗(yàn)結(jié)果重復(fù)性更好的CLP造模方法,可推廣應(yīng)用于對膿毒癥機(jī)制的研究以及對藥物療效進(jìn)行評估。
[Abstract]:Objective: the best animal model to simulate clinical sepsis is the (CLP) model of rodent cecal ligation and perforation. The main method is to control the thickness of cecum puncture needle. The number of perforations and the length of cecal ligation were used to control the mortality of experimental animals. The purpose of this study was to establish a CLP model based on measuring the volume of cecum to further control the severity and mortality of animal infection, and to simulate the CLP model based on measuring the length of cecum in previous literatures for comparison. To explore a feasible scheme to improve the stability of the model. Methods: the model of cecal ligation and perforation was established in male SD rats. The model was divided into two groups: length mapping ligation and volume mapping ligation. The length of length from ileocecal flap to cecum apical was measured by silk line in length mapping group. Cecum was ligated by different length ratio (50% and 75% of the total length of cecum). The volume of cecum was labeled with methylene blue in the volume mapping group, and the cecum was ligated in different volume groups (0.5 ml, 1.5 ml, 2.0ml and 2.5ml). After ligation, the perforated caecum was single perforated with needle 21. The blood culture and peritoneal secretion smear culture were performed 6 hours after the operation and the death rate was observed for 7 days. Results: the 7-day mortality of 50% and 75% of the ligation group was 90% and 100%, respectively. There was no significant difference between the two groups (n = 10, p = 0.661). The mortality in the group of volume mapping and ligation increased with the increase of the volume of the cecal ligation. The mortality rates of 0.5 ml of 1.0 ml of 1. 0 ml 1. 5 ml of 1. 5 ml and 2. 0 ml of 2.5ml for 7 days were 40% and 50%, 80% and 100%, respectively. There was a significant difference among the three groups (n = 10, p = 0.002), but all of the sham operation groups survived. The mean survival time of 50% and 75% subgroups was 1.621 鹵0.605 days and 1.150 鹵0.099 days, respectively. The mean survival time in the volume mapping ligation group ranged from 4.708 鹵0.893 days in the 0.5ml ligation subgroup to 1.175 鹵0.240 days in the 2.5ml ligation subgroup. 6 hours after CLP, blood culture and peritoneal secretion smear culture were positive except the sham operation group. Conclusion: the operative procedure and experimental data show that the method of volume ligation and perforation of the caecum is a more convenient and standardized procedure, and it is a more reproducible CLP model method. It can be used to study the mechanism of sepsis and evaluate the efficacy of drugs.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R459.7;R-332

【共引文獻(xiàn)】

相關(guān)期刊論文 前3條

1 郭媛;魏筱華;謝s，

本文編號：2339426