大鼠異位節(jié)段小腸移植模型建立技術(shù)的改進(jìn)
發(fā)布時(shí)間:2018-10-26 11:55
【摘要】:目的:建立大鼠異位節(jié)段小腸移植模型。方法:對(duì)40例(80只)Wistar大鼠施行異位小腸移植,供受體術(shù)前抗生素灌胃,改變供受體術(shù)式,減少受體手術(shù)時(shí)間、手術(shù)損傷及供腸缺血時(shí)間;采用腹主動(dòng)脈-腸系膜上動(dòng)脈吻合以及左腎靜脈-門靜脈單套管吻合,血管吻合方法采用單純間斷吻合,重建供腸血管;移植小腸雙造口,靜脈補(bǔ)液通路采用股靜脈。結(jié)果:腸缺血時(shí)間≤35min,吻合口無(wú)狹窄,40例大鼠接受小腸移植,建模成功35例。結(jié)論:改進(jìn)小腸移植技術(shù)中的多個(gè)細(xì)節(jié)后,降低了大鼠小腸移植術(shù)的難度。
[Abstract]:Objective: to establish an ectopic segment small bowel transplantation model in rats. Methods: 40 cases (80) of Wistar rats were treated with ectopic small bowel transplantation. The donor recipients were perfused with antibiotics before operation to change the operation mode of the donor receptors and to reduce the time of operation, injury and ischemia of the donor intestine. Abdominal aorta-superior mesenteric artery anastomosis and single cannula anastomosis of left renal vein and portal vein were used. Results: intestinal ischemia time 鈮,
本文編號(hào):2295632
[Abstract]:Objective: to establish an ectopic segment small bowel transplantation model in rats. Methods: 40 cases (80) of Wistar rats were treated with ectopic small bowel transplantation. The donor recipients were perfused with antibiotics before operation to change the operation mode of the donor receptors and to reduce the time of operation, injury and ischemia of the donor intestine. Abdominal aorta-superior mesenteric artery anastomosis and single cannula anastomosis of left renal vein and portal vein were used. Results: intestinal ischemia time 鈮,
本文編號(hào):2295632
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