HIF-1α在失血性動物心肌中的表達
發(fā)布時間：2018-10-26 10:31
【摘要】：目的:探討不同程度失血大鼠心肌細胞中缺氧誘導因子-1ɑ(hypoxia-inducible factor-1ɑ)蛋白和mRNA的表達和意義。 方法:選用清潔級SD大鼠,建立不同程度的失血模型,隨機分為對照組(CON),失血10%(10-BL),失血20%(20-BL),失血30%(30-BL),利用Powerlab八道生理記錄儀監(jiān)測心功能指標,采用RT-PCR及Western Blot檢測HIF-1ɑm RNA及蛋白在失血后30min,1h大鼠心肌組織中的表達情況,并用免疫組化染色檢測HIF-1ɑ在心肌細胞中的分布。 結果:不同程度失血后,心功能均降低,1h比30min降低明顯(P0.05);RT-PCR半定量結果顯示,與CON組相比,10-BL、20-BL、30-BL組HIF-1ɑmRNA表達逐漸增高(P0.05);免疫組化結果表明,HIF-1α主要分布在心肌細胞細胞質中,在細胞核內也有分布。 結論:隨失血程度增大,時間延長,HIF-1α表達增加,HIF-1α可能參與失血導致的低氧狀態(tài)下心肌代謝的適應性調節(jié)。 目的:探討不同程度失血后豬心肌細胞中缺氧誘導因子-1ɑ(hypoxia-inducible factor-1ɑ)蛋白和mRNA的表達和意義。 方法:選用小白豬,建立不同程度的失血模型,隨機分為對照組(CON),失血10%(10-BL),失血20%(20-BL),失血30%(30-BL),利用Powerlab八道生理記錄儀監(jiān)測心功能指標,采用RT-PCR及Western Blot檢測HIF-1ɑmRNA及蛋白在失血后1h小白豬心肌組織中的表達情況,并用免疫組化染色檢測HIF-1ɑ在心肌細胞中的分布。 結果:不同程度失血后,心功能指標降低;RT-PCR半定量結果顯示,與CON組相比,10-BL、20-BL、30-BL組HIF-1ɑmRNA表達逐漸增高(P0.05);免疫組化結果表明,HIF-1α主要分布在心肌細胞細胞質中,在細胞核內也有分布。 結論:失血程度增大,時間延長,HIF-1α表達增加,HIF-1α可能參與失血導致的低氧狀態(tài)下心肌代謝的適應性調節(jié)。
[Abstract]:Aim: to investigate the expression and significance of hypoxia inducible factor-1 (hypoxia-inducible factor-1) protein and mRNA in cardiac myocytes of rats with different degrees of blood loss. Methods: blood loss models were established in clean grade SD rats. The rats were randomly divided into three groups: (CON), 10% (10-BL), bleeding 20% (20-BL), and bleeding 30% (30-BL). The cardiac function was monitored by Powerlab eight-channel physiological recorder, and the expression of HIF-1 m RNA and protein was detected by RT-PCR and Western Blot in the myocardium of rats 30 min after blood loss. The distribution of HIF-1 in cardiomyocytes was detected by immunohistochemical staining. Results: compared with CON group, the expression of HIF-1 mRNA in 10-BL20-BL 30-BL group was increased gradually (P0.05), and the results of RT-PCR semi-quantitative analysis showed that the cardiac function decreased significantly at 1 hour after different degree of blood loss (P 0.05), and the expression of HIF-1 mRNA in 10-BL20-BL 30-BL group was significantly higher than that in CON group (P0.05). Immunohistochemical results showed that HIF-1 偽 was mainly distributed in the cytoplasm of myocardium and also in the nucleus. Conclusion: with the increase of blood loss, the expression of HIF-1 偽 is increased. HIF-1 偽 may be involved in the adaptive regulation of myocardial metabolism in hypoxic state induced by blood loss. Aim: to investigate the expression and significance of hypoxia inducible factor-1 (hypoxia-inducible factor-1) protein and mRNA in porcine cardiac myocytes after different degrees of blood loss. Methods: a blood loss model was established in small white pigs. The model was randomly divided into three groups: 10% (10-BL), 20% (20-BL), and 30% (30-BL) of (CON), in control group. The cardiac function was monitored by Powerlab eight-channel physiological recorder. The expression of HIF-1 mRNA and protein was detected by RT-PCR and Western Blot, and the distribution of HIF-1 in cardiac myocytes was detected by immunohistochemical staining. Results: after different degree of blood loss, cardiac function index decreased, RT-PCR semi-quantitative results showed that compared with CON group, the expression of HIF-1 mRNA increased gradually in 10-BL20-BL 30-BL group (P0.05). Immunohistochemical results showed that HIF-1 偽 was mainly distributed in the cytoplasm of myocardium and also in the nucleus. Conclusion: the degree of blood loss is increased, the time is prolonged and the expression of HIF-1 偽 is increased. HIF-1 偽 may be involved in the adaptive regulation of myocardial metabolism in hypoxic state induced by blood loss.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R363
本文編號：2295428
[Abstract]:Aim: to investigate the expression and significance of hypoxia inducible factor-1 (hypoxia-inducible factor-1) protein and mRNA in cardiac myocytes of rats with different degrees of blood loss. Methods: blood loss models were established in clean grade SD rats. The rats were randomly divided into three groups: (CON), 10% (10-BL), bleeding 20% (20-BL), and bleeding 30% (30-BL). The cardiac function was monitored by Powerlab eight-channel physiological recorder, and the expression of HIF-1 m RNA and protein was detected by RT-PCR and Western Blot in the myocardium of rats 30 min after blood loss. The distribution of HIF-1 in cardiomyocytes was detected by immunohistochemical staining. Results: compared with CON group, the expression of HIF-1 mRNA in 10-BL20-BL 30-BL group was increased gradually (P0.05), and the results of RT-PCR semi-quantitative analysis showed that the cardiac function decreased significantly at 1 hour after different degree of blood loss (P 0.05), and the expression of HIF-1 mRNA in 10-BL20-BL 30-BL group was significantly higher than that in CON group (P0.05). Immunohistochemical results showed that HIF-1 偽 was mainly distributed in the cytoplasm of myocardium and also in the nucleus. Conclusion: with the increase of blood loss, the expression of HIF-1 偽 is increased. HIF-1 偽 may be involved in the adaptive regulation of myocardial metabolism in hypoxic state induced by blood loss. Aim: to investigate the expression and significance of hypoxia inducible factor-1 (hypoxia-inducible factor-1) protein and mRNA in porcine cardiac myocytes after different degrees of blood loss. Methods: a blood loss model was established in small white pigs. The model was randomly divided into three groups: 10% (10-BL), 20% (20-BL), and 30% (30-BL) of (CON), in control group. The cardiac function was monitored by Powerlab eight-channel physiological recorder. The expression of HIF-1 mRNA and protein was detected by RT-PCR and Western Blot, and the distribution of HIF-1 in cardiac myocytes was detected by immunohistochemical staining. Results: after different degree of blood loss, cardiac function index decreased, RT-PCR semi-quantitative results showed that compared with CON group, the expression of HIF-1 mRNA increased gradually in 10-BL20-BL 30-BL group (P0.05). Immunohistochemical results showed that HIF-1 偽 was mainly distributed in the cytoplasm of myocardium and also in the nucleus. Conclusion: the degree of blood loss is increased, the time is prolonged and the expression of HIF-1 偽 is increased. HIF-1 偽 may be involved in the adaptive regulation of myocardial metabolism in hypoxic state induced by blood loss.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R363
【參考文獻】
相關期刊論文 前10條
1 余俐,徐如祥,朱紅勝,郭再玉,周青,杜謀選,陳鎮(zhèn)洲,謝培增,王松青,彭蘋,簡衛(wèi)紅;高溫高濕環(huán)境下顱腦火器傷對狗心肌組織的損害作用[J];中華神經醫(yī)學雜志;2004年01期
2 于勇,付小兵,周寶桐,晉樺,孫同柱,黃宇宏,周貴生,周曉光,李興利,王麗云,趙玉杰,譚廣華;低溫環(huán)境下槍彈傷感染的實驗研究[J];創(chuàng)傷外科雜志;2001年02期
3 張金洲,易定華,蔡建輝;胸部火器傷后心臟功能改變[J];第四軍醫(yī)大學學報;1999年01期
4 蔡建輝,劉維永,郭建軍,張金洲,李宏;犬胸腔槍彈傷模型的建立及早期救治[J];第四軍醫(yī)大學學報;2000年05期
5 李同勛,劉維永,蔡建輝,陶登順,李宏;家兔右胸火器穿透傷的出血來源[J];第四軍醫(yī)大學學報;2000年05期
6 陶登順,劉維永,蔡建輝,李宏,李同勛,郭建軍,安書杰;兔胸部槍傷瞬時及早期心功能的變化[J];第四軍醫(yī)大學學報;2000年05期
7 郭建軍,劉維永,盧春蓉,劉慧玲;家兔胸部火器傷后各組織中TNF-α表達意義[J];第四軍醫(yī)大學學報;2001年15期
8 陶登順,劉維永,蔡建輝,李宏,李同勛,郭建軍,安書杰;兔胸部槍傷后血漿一氧化氮及β-內啡肽含量與早期心血管功能變化[J];第四軍醫(yī)大學學報;2002年06期
9 陶登順,劉維永,蔡建輝,李宏,李同勛,郭建軍,安書杰;切斷迷走神經和減壓神經對胸部火器傷心血管功能效應[J];第四軍醫(yī)大學學報;2004年02期
10 侯明山;李茂林;馬蘭香;劉西梅;王祝峰;;犬顱腦槍彈傷后心肌細胞凋亡及bcl-2的表達[J];第四軍醫(yī)大學學報;2007年17期
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