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HIF-1α在失血性動物心肌中的表達

發(fā)布時間:2018-10-26 10:31
【摘要】:目的:探討不同程度失血大鼠心肌細胞中缺氧誘導因子-1ɑ(hypoxia-inducible factor-1ɑ)蛋白和mRNA的表達和意義。 方法:選用清潔級SD大鼠,建立不同程度的失血模型,隨機分為對照組(CON),失血10%(10-BL),失血20%(20-BL),失血30%(30-BL),利用Powerlab八道生理記錄儀監(jiān)測心功能指標,采用RT-PCR及Western Blot檢測HIF-1ɑm RNA及蛋白在失血后30min,1h大鼠心肌組織中的表達情況,并用免疫組化染色檢測HIF-1ɑ在心肌細胞中的分布。 結果:不同程度失血后,心功能均降低,1h比30min降低明顯(P0.05);RT-PCR半定量結果顯示,與CON組相比,10-BL、20-BL、30-BL組HIF-1ɑmRNA表達逐漸增高(P0.05);免疫組化結果表明,HIF-1α主要分布在心肌細胞細胞質中,在細胞核內也有分布。 結論:隨失血程度增大,時間延長,HIF-1α表達增加,HIF-1α可能參與失血導致的低氧狀態(tài)下心肌代謝的適應性調節(jié)。 目的:探討不同程度失血后豬心肌細胞中缺氧誘導因子-1ɑ(hypoxia-inducible factor-1ɑ)蛋白和mRNA的表達和意義。 方法:選用小白豬,建立不同程度的失血模型,隨機分為對照組(CON),失血10%(10-BL),失血20%(20-BL),失血30%(30-BL),利用Powerlab八道生理記錄儀監(jiān)測心功能指標,采用RT-PCR及Western Blot檢測HIF-1ɑmRNA及蛋白在失血后1h小白豬心肌組織中的表達情況,并用免疫組化染色檢測HIF-1ɑ在心肌細胞中的分布。 結果:不同程度失血后,心功能指標降低;RT-PCR半定量結果顯示,與CON組相比,10-BL、20-BL、30-BL組HIF-1ɑmRNA表達逐漸增高(P0.05);免疫組化結果表明,HIF-1α主要分布在心肌細胞細胞質中,在細胞核內也有分布。 結論:失血程度增大,時間延長,HIF-1α表達增加,HIF-1α可能參與失血導致的低氧狀態(tài)下心肌代謝的適應性調節(jié)。
[Abstract]:Aim: to investigate the expression and significance of hypoxia inducible factor-1 (hypoxia-inducible factor-1) protein and mRNA in cardiac myocytes of rats with different degrees of blood loss. Methods: blood loss models were established in clean grade SD rats. The rats were randomly divided into three groups: (CON), 10% (10-BL), bleeding 20% (20-BL), and bleeding 30% (30-BL). The cardiac function was monitored by Powerlab eight-channel physiological recorder, and the expression of HIF-1 m RNA and protein was detected by RT-PCR and Western Blot in the myocardium of rats 30 min after blood loss. The distribution of HIF-1 in cardiomyocytes was detected by immunohistochemical staining. Results: compared with CON group, the expression of HIF-1 mRNA in 10-BL20-BL 30-BL group was increased gradually (P0.05), and the results of RT-PCR semi-quantitative analysis showed that the cardiac function decreased significantly at 1 hour after different degree of blood loss (P 0.05), and the expression of HIF-1 mRNA in 10-BL20-BL 30-BL group was significantly higher than that in CON group (P0.05). Immunohistochemical results showed that HIF-1 偽 was mainly distributed in the cytoplasm of myocardium and also in the nucleus. Conclusion: with the increase of blood loss, the expression of HIF-1 偽 is increased. HIF-1 偽 may be involved in the adaptive regulation of myocardial metabolism in hypoxic state induced by blood loss. Aim: to investigate the expression and significance of hypoxia inducible factor-1 (hypoxia-inducible factor-1) protein and mRNA in porcine cardiac myocytes after different degrees of blood loss. Methods: a blood loss model was established in small white pigs. The model was randomly divided into three groups: 10% (10-BL), 20% (20-BL), and 30% (30-BL) of (CON), in control group. The cardiac function was monitored by Powerlab eight-channel physiological recorder. The expression of HIF-1 mRNA and protein was detected by RT-PCR and Western Blot, and the distribution of HIF-1 in cardiac myocytes was detected by immunohistochemical staining. Results: after different degree of blood loss, cardiac function index decreased, RT-PCR semi-quantitative results showed that compared with CON group, the expression of HIF-1 mRNA increased gradually in 10-BL20-BL 30-BL group (P0.05). Immunohistochemical results showed that HIF-1 偽 was mainly distributed in the cytoplasm of myocardium and also in the nucleus. Conclusion: the degree of blood loss is increased, the time is prolonged and the expression of HIF-1 偽 is increased. HIF-1 偽 may be involved in the adaptive regulation of myocardial metabolism in hypoxic state induced by blood loss.
【學位授予單位】:重慶醫(yī)科大學
【學位級別】:碩士
【學位授予年份】:2011
【分類號】:R363

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