【摘要】：目的:探討簡便有效的腎陽虛建模方法及對大鼠的影響,為方劑藥效學(xué)及機制研究奠定基礎(chǔ)。方法:用腺嘌呤皮下注射和氫化可的松肌肉注射構(gòu)建腎陽虛大鼠模型,觀察大鼠表現(xiàn)及腎臟睪丸病理變化,測定其臟器指數(shù)、自主活動度、游泳時間、血清尿素(SU)、血清肌酐(Cr)、SOD和MDA活力。結(jié)果:與空白組相比,兩種建模方法均使大鼠自主活動度和游泳時間降低(P0.05),腎臟指數(shù)和睪丸指數(shù)增高(P0.05);腺嘌呤組大鼠的Cr水平、SOD活力和MDA活力均升高(P0.05);氫化可的松組大鼠的Cr水平和SOD活力降低(P0.05)、MDA活力升高(P0.05);病理變化上,兩種建模方法均出現(xiàn)炎性細胞浸潤腎間質(zhì)、睪丸生精小管略萎縮,排列疏松,各級生精細胞排列松散等現(xiàn)象。結(jié)論:腺嘌呤與氫化可的松構(gòu)建腎陽虛模型在虛證表現(xiàn)和腎臟睪丸病變上相似,但腺嘌呤可能是通過損害腎濾過水平、睪丸生理功能等引起Cr、SOD和MDA活力升高,氫化可的松則可能是通過耗散機體能量,減弱機體抵抗能力,影響Cr生成、降低SOD活力和增高MDA活力,具體原因有待進一步探究。
[Abstract]:Objective: to explore a simple and effective modeling method of kidney-yang deficiency and its effect on rats, so as to lay a foundation for the pharmacodynamics and mechanism of prescription. Methods: the rat model of kidney yang deficiency was established by subcutaneous injection of adenine and intramuscular injection of hydrocortisone. Serum urea (SU), serum creatinine (Cr), SOD and MDA activity. Results: compared with the blank group, Both modeling methods decreased autonomous activity and swimming time of rats (P0.05), increased renal index and testicular index (P0.05), increased Cr level, SOD activity and MDA activity of rats in adenine group (P0.05); Cr level and SOD level in hydrocortisone group rats. The activity of), MDA was decreased (P0.05), the activity of), MDA was increased (P0.05), and the pathological changes were observed. The inflammatory cells infiltrated the renal interstitial cells, the testicular seminiferous tubules shrank slightly, the arrangement of spermatogenic cells was loose, and so on. Conclusion: the kidney yang deficiency model constructed by adenine and hydrocortisone is similar in deficiency syndrome and renal testicular lesion, but adenine may cause the increase of Cr,SOD and MDA activity by damaging renal filtration level and testicular physiological function. Hydrocortisone may dissipate body energy, weaken body resistance, affect Cr production, decrease SOD activity and increase MDA activity. The specific reasons need to be further explored.
【作者單位】： 廣州中醫(yī)藥大學(xué);廣州中醫(yī)藥大學(xué)附屬寶安中醫(yī)院;
【基金】：國家自然科學(xué)基金資助項目(81102714) 國家級大學(xué)生創(chuàng)新訓(xùn)練項目(201410572026) 廣東省大學(xué)生創(chuàng)新訓(xùn)練項目(201410572077)
【分類號】：R285.5;R-332

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