【摘要】：目的:探討左歸丸對腎陰虛模型大鼠神經(jīng)-內(nèi)分泌-免疫功能的作用機制。方法:按隨機數(shù)字將60只清潔級健康雄性Wistar大鼠分均為正常組(同體積生理鹽水),模型組(同體積生理鹽水),預(yù)防組(與造模同時,1 g·kg~(-1)左歸丸)及左歸丸低劑量組(造模后,0.5 g·kg~(-1)左歸丸),中劑量組(造模后,1 g·kg~(-1)左歸丸),高劑量組(造模后,2 g·kg~(-1)左歸丸),以甲狀腺片灌胃制備腎陰虛模型;所有組均連續(xù)灌胃21 d。采集脾、胸腺、尿液和血液樣本,計算臟器指數(shù);采用酶聯(lián)免疫吸附法(ELISA)檢測促腎上腺皮質(zhì)素釋放激素(CRH),促腎上腺皮質(zhì)激素(ACTH),皮質(zhì)醇(CORT),三碘甲狀腺原氨酸(T3),四碘甲狀腺原氨酸(T4),甲狀腺刺激激素(TSH),環(huán)磷酸腺苷(c AMP),環(huán)磷酸鳥苷(c GMP),17-羥皮質(zhì)類固醇(17-OHCS),白細胞介素-6(IL-6),干擾素-γ(IFN-γ)水平。結(jié)果:與正常組比較,模型組的T3,T4,c AMP,c GMP,IL-6,IFN-γ明顯升高,TSH,c AMP/c GMP,脾指數(shù),胸腺指數(shù)降低,差異顯著(P0.05,P0.01),預(yù)防組各指標無明顯變化。與模型組比較,左歸丸各劑量組的T3,T4,c AMP,c GMP,IL-6,IFN-γ明顯降低,TSH,c AMP/c GMP,脾指數(shù)、胸腺指數(shù)明顯升高,差異顯著(P0.05,P0.01);預(yù)防組T3,T4,CRH,ACTH,CORT,c AMP,c GMP,17-OHCS,脾指數(shù),胸腺指數(shù)明顯升高(P0.01),TSH,c AMP/c GMP,IL-6,IFN-γ明顯降低(P0.01)。與預(yù)防組比較,左歸丸各劑量組的T3,CRH,ACTH較高(P0.05,P0.01)。結(jié)論:左歸丸能調(diào)節(jié)甲亢型腎陰虛大鼠下丘腦-垂體-腎上腺軸和下丘腦-垂體-甲狀腺軸功能,改善免疫功能,這可能是干預(yù)甲亢型腎陰虛作用的機制之一。
[Abstract]:Objective: to investigate the mechanism of Zuogui Pill on neuroendocrine-immune function in rats with kidney yin deficiency. Methods: sixty clean grade healthy male Wistar rats were randomly divided into normal group (normal saline of the same volume), model group (same volume of saline), prevention group (1 g kg~ (-1) Zuogui pill) and low dose of Zuogui pill. The model group (0.5 g kg~ (-1) Zuo Gui Wan), the middle dose group (1 g kg~ (-1) Zuo Gui Pill) and the high dose group (2 g kg~ (-1) Zuo Gui Pill) were perfused with thyroid tablets to establish the model of kidney yin deficiency. All the groups were perfused continuously for 21 days. Spleen thymus urine and blood samples were collected to calculate the visceral index. Enzyme linked immunosorbent assay (ELISA) for the detection of adrenocorticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), cortisol, (CORT), triiodothyronine (T3), tetraiodothyronine (T4), and thyroid stimulating hormone (TSH), cyclic adenosine phosphate (c AMP),) The levels of (c GMP), 17-hydroxycorticosteroid (17-OHCS), interleukin-6 (IL-6) and interferon- 緯 (IFN- 緯) were measured. Results: compared with the normal group, the T _ (3) T _ (4) C AMP,c GMP,IL-6,IFN- 緯 in the model group was significantly higher than that in the control group, but the spleen index and thymus index of the model group were decreased significantly (P 0.05 ~ (0.01), but no significant change was found in the prevention group. Compared with the model group, the T _ (3) T _ (4) C AMP,c GMP,IL-6,IFN- 緯, TSH,c AMP/c GMP, spleen index and thymus index in each dose group were significantly lower than those in the model group (P < 0.05), while in the prevention group, the spleen index, thymus index and TSH,c AMP/c GMP,IL-6,IFN- 緯 were significantly increased (P ~ (0.01) and significantly decreased (P ~ (0.01). Compared with the prevention group, the T _ 3 CRH ACTH of each dose group was higher than that of the prevention group (P 0.05? P 0.01). Conclusion: Zuogui Pill can regulate the function of hypothalamus-pituitary-adrenal axis and hypothalamus-pituitary-thyroid axis and improve immune function in hyperthyroidism rats.
【作者單位】： 黃河中心醫(yī)院;
【基金】：河南省醫(yī)學(xué)科技攻關(guān)計劃項目(201404015)
【分類號】：R285.5;R-332

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本文編號：2277699