憤怒郁怒情緒反應及其誘發(fā)PMS病證結(jié)合獼猴模型的建立及評價
發(fā)布時間：2018-10-17 17:58
【摘要】：目的:完成制備憤怒郁怒情緒反應及其誘發(fā)PMS肝氣逆、肝氣郁證獼猴模型的制備及評價,為探索憤怒郁怒致病機理提供適宜的載體平臺;檢測獼猴月經(jīng)周期不同時期血清及中樞神經(jīng)遞質(zhì)含量變化,初步揭示憤怒郁怒情緒誘發(fā)情志病證的發(fā)病機制;以中藥經(jīng)前平和經(jīng)前舒顆粒對PMS模型獼猴進行干預,為闡明其作用機理提供有價值的線索。 方法:應用社會層級壓力應激法(Social Subordination Stress)誘導獼猴出現(xiàn)情緒反應,然后依據(jù)《雌性實驗獼猴情緒評價量表》(以下簡稱“量表”)評分配合實驗獼猴面部表情計算機模式識別系統(tǒng)(以下簡稱“模式識別系統(tǒng)”)篩選出憤怒郁怒情緒反應獼猴模型,選取上述獼猴配合擇時擠壓造模使獼猴出現(xiàn)PMS肝氣逆、肝氣郁表現(xiàn),隨后采用量表評分和模式識別系統(tǒng)輔助判別其表情行為,最后應用中藥經(jīng)前平顆粒對PMS肝氣逆證、經(jīng)前舒顆粒對PMS肝氣郁證進行干預。采集月經(jīng)周期不同時期獼猴血清,分析血清和不同腦區(qū)NE、DA、5-HT和GABA(僅腦區(qū))含量,結(jié)合量表評分結(jié)果,輔助模型評價,反證造模效果。 結(jié)果:憤怒、郁怒情緒反應獼猴憤怒因子積分均顯著高于對照組(P0.01),憤怒情緒組又顯著高于郁怒情緒組(P0.05),郁怒情緒反應獼猴抑郁因子積分顯著高于對照組和憤怒情緒反應組(P0.01),經(jīng)前平和經(jīng)前舒顆�？娠@著降低PMS肝氣逆、郁證獼猴憤怒、抑郁因子積分(P0.05)。與對照組和情緒反應組相比,PMS肝氣逆、郁證造模組血清單胺類神經(jīng)遞質(zhì)含量顯著升高(P0.05, P0.01),經(jīng)前平和經(jīng)前舒顆粒給藥后均顯著下降(P0.05, P0.01)。相較于對照組,PMS肝氣逆證造模組垂體DA、下丘腦NE、DA,頂區(qū)皮質(zhì)NE、DA、5-HT上升(P0.05, P0.01),垂體NE、GABA下降(P0.05, P0.01);PMS肝氣郁證造模組垂體DA、GABA,下丘腦和頂區(qū)皮質(zhì)NE、DA、5-HT,額區(qū)皮質(zhì)GABA升高(P0.05, P0.01),垂體NE,GABA,垂體、下丘腦和頂區(qū)皮質(zhì)GABA含量降低(P0.05, P0.01)。 結(jié)論:社會層級壓力應激法造模能較好誘導出實驗獼猴出現(xiàn)憤怒郁怒情緒,主觀觀測、量表評分及神經(jīng)遞質(zhì)檢測結(jié)果可作為評價實驗獼猴憤怒郁怒情緒及病癥表現(xiàn)的較好測量方法。憤怒郁怒情緒與血清單胺類神經(jīng)遞質(zhì)變化有關,PMS肝氣逆、郁證發(fā)病與血清及中樞單胺類神經(jīng)遞質(zhì)和中樞GABA含量密切相關。經(jīng)前平和經(jīng)前舒顆粒調(diào)整血清及中樞神經(jīng)遞質(zhì)紊亂可能是其分別用于治療PMS肝氣逆、郁證的重要作用機制。
[Abstract]:Objective: to prepare and evaluate the model of anger depression and anger emotional response and its inducing PMS liver qi inversion and liver qi depression syndrome in rhesus monkeys, and to provide a suitable carrier platform for exploring the pathogenesis of anger depression and anger. The changes of serum and central neurotransmitters in different periods of menstrual cycle of rhesus monkey were detected, and the pathogenesis of emotion syndrome induced by anger depression and anger was revealed, and the intervention of Jingqian Jingqianshu granule on PMS model rhesus monkey was carried out. It provides valuable clues for elucidating the mechanism of action. Methods: (Social Subordination Stress) was used to induce emotional response in rhesus monkeys. Then according to the "female Experimental Rhesus Monkey emotional Evaluation scale" (hereinafter referred to as "the scale") score combined with the experimental rhesus monkey facial expression computer pattern recognition system (hereinafter referred to as the "pattern recognition system") to screen anger depression anger The rhesus monkey model of thread reaction, The rhesus macaque was selected to make the rhesus monkey with the time and compression model to make the rhesus monkey appear PMS liver-qi inverse and liver-qi depression, then the scale score and the pattern recognition system were used to judge the expression behavior of the rhesus monkey. Finally, the Chinese medicine Jingqian Ping granule was applied to the PMS liver-qi inverse syndrome. Jingqianshu granule intervention on PMS liver qi stagnation syndrome. The rhesus monkey serum was collected in different periods of menstrual cycle, and the contents of NE,DA,5-HT and GABA in serum and different brain regions were analyzed. The result: anger, The scores of anger factor in rhesus monkey were significantly higher than those in control group (P0.01). The score of depression factor in anger group was significantly higher than that in depression emotion group (P0.05). The score of depression factor in rhesus monkey was significantly higher than that in control group and angry emotion response group (P0.01). Qianping Jingqianshu granule could significantly reduce the liver qi inverse of PMS. Anger and depression factor score of rhesus monkey with depression syndrome (P0.05). Compared with the control group and the emotional response group, the contents of amine neurotransmitters in blood list of PMS liver qi inversion group and depression syndrome model group were significantly increased (P0.05, P0.01), and all of them were significantly decreased (P0.05, P0.01) after administration of Qianqianshu granule (P0.05, P0.01). Compared with the control group, the NE,DA,5-HT of pituitary DA, in the hypothalamic NE,DA, parietal cortex increased (P0.05, P0.01), the pituitary NE,GABA decreased (P0.05, P0.01); PMS, P0.01); PMS liver qi depression group) in the model group, the GABA in the NE,DA,5-HT, frontal area of the hypothalamus and the parietal cortex in the model group was increased (P0.05, P0.01), and the pituitary NE,GABA, pituitary was increased in the model group (P0.05, P0.01). The content of GABA in hypothalamus and parietal cortex decreased (P 0.05, P 0.01). Conclusion: the model of stress at social level can induce anger and anger in experimental rhesus monkeys, and can be observed subjectively. The scale score and neurotransmitter test results can be used as a better method to evaluate the anger depression and symptoms of rhesus monkeys. Anger and anger were related to the changes of blood list amine neurotransmitters. The changes of serum and central monoamine neurotransmitters and central GABA were closely related to the liver qi inverses of PMS and the pathogenesis of depression syndrome. Regulating the disorder of serum and central neurotransmitter by Jingqingjingqianshu granule may be the important mechanism of its application in the treatment of liver qi inverses and depression syndrome in PMS.
【學位授予單位】：山東中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R-332
本文編號：2277496
[Abstract]:Objective: to prepare and evaluate the model of anger depression and anger emotional response and its inducing PMS liver qi inversion and liver qi depression syndrome in rhesus monkeys, and to provide a suitable carrier platform for exploring the pathogenesis of anger depression and anger. The changes of serum and central neurotransmitters in different periods of menstrual cycle of rhesus monkey were detected, and the pathogenesis of emotion syndrome induced by anger depression and anger was revealed, and the intervention of Jingqian Jingqianshu granule on PMS model rhesus monkey was carried out. It provides valuable clues for elucidating the mechanism of action. Methods: (Social Subordination Stress) was used to induce emotional response in rhesus monkeys. Then according to the "female Experimental Rhesus Monkey emotional Evaluation scale" (hereinafter referred to as "the scale") score combined with the experimental rhesus monkey facial expression computer pattern recognition system (hereinafter referred to as the "pattern recognition system") to screen anger depression anger The rhesus monkey model of thread reaction, The rhesus macaque was selected to make the rhesus monkey with the time and compression model to make the rhesus monkey appear PMS liver-qi inverse and liver-qi depression, then the scale score and the pattern recognition system were used to judge the expression behavior of the rhesus monkey. Finally, the Chinese medicine Jingqian Ping granule was applied to the PMS liver-qi inverse syndrome. Jingqianshu granule intervention on PMS liver qi stagnation syndrome. The rhesus monkey serum was collected in different periods of menstrual cycle, and the contents of NE,DA,5-HT and GABA in serum and different brain regions were analyzed. The result: anger, The scores of anger factor in rhesus monkey were significantly higher than those in control group (P0.01). The score of depression factor in anger group was significantly higher than that in depression emotion group (P0.05). The score of depression factor in rhesus monkey was significantly higher than that in control group and angry emotion response group (P0.01). Qianping Jingqianshu granule could significantly reduce the liver qi inverse of PMS. Anger and depression factor score of rhesus monkey with depression syndrome (P0.05). Compared with the control group and the emotional response group, the contents of amine neurotransmitters in blood list of PMS liver qi inversion group and depression syndrome model group were significantly increased (P0.05, P0.01), and all of them were significantly decreased (P0.05, P0.01) after administration of Qianqianshu granule (P0.05, P0.01). Compared with the control group, the NE,DA,5-HT of pituitary DA, in the hypothalamic NE,DA, parietal cortex increased (P0.05, P0.01), the pituitary NE,GABA decreased (P0.05, P0.01); PMS, P0.01); PMS liver qi depression group) in the model group, the GABA in the NE,DA,5-HT, frontal area of the hypothalamus and the parietal cortex in the model group was increased (P0.05, P0.01), and the pituitary NE,GABA, pituitary was increased in the model group (P0.05, P0.01). The content of GABA in hypothalamus and parietal cortex decreased (P 0.05, P 0.01). Conclusion: the model of stress at social level can induce anger and anger in experimental rhesus monkeys, and can be observed subjectively. The scale score and neurotransmitter test results can be used as a better method to evaluate the anger depression and symptoms of rhesus monkeys. Anger and anger were related to the changes of blood list amine neurotransmitters. The changes of serum and central monoamine neurotransmitters and central GABA were closely related to the liver qi inverses of PMS and the pathogenesis of depression syndrome. Regulating the disorder of serum and central neurotransmitter by Jingqingjingqianshu granule may be the important mechanism of its application in the treatment of liver qi inverses and depression syndrome in PMS.
【學位授予單位】：山東中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R-332
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