【摘要】：目的:研究黃芪桂枝五物湯(HGW)對膠原誘導(dǎo)型關(guān)節(jié)炎(CIA)模型大鼠關(guān)節(jié)滑膜細(xì)胞凋亡的影響,并探討其作用機(jī)制。方法:選用SD大鼠72只,隨機(jī)分為正常組,模型組,HGW低、中、高劑量組,Tofacitinib(TFN)組,HGW低、中、高劑量組藥物劑量分別為16,32,64 g·kg~(-1),相當(dāng)于臨床常人用量的3,6,12倍,TFN給藥劑量為15 mg·kg~(-1),正常組、模型組每日給予10 m L·kg~(-1)蒸餾水。以牛Ⅱ型膠原加弗氏完全佐劑建立CIA大鼠模型,經(jīng)HGW治療4周后光鏡下觀察各組大鼠踝關(guān)節(jié)病理形態(tài);通過流式細(xì)胞術(shù)檢測白細(xì)胞介素(IL)-4,IL~(-1)0含量,免疫組化法檢測B淋巴細(xì)胞瘤-2(B-cell lymphoma-2,Bcl-2),Bcl-2相關(guān)X蛋白(Bax)蛋白表達(dá),DNA斷裂的原位末端標(biāo)記法(TUNEL)染色檢測滑膜細(xì)胞的凋亡。結(jié)果:與正常組比較,模型組,HGW低、中、高劑量組,TFN組大鼠滑膜中Bcl-2蛋白表達(dá)增多(P0.05),模型組Bax蛋白表達(dá)減少(P0.05);與模型組比較,HGW高劑量組,TFN組大鼠滑膜中Bcl-2蛋白表達(dá)減少,Bax蛋白表達(dá)增多(P0.05)。滑膜細(xì)胞凋亡可見,與正常組比較,模型組,HGW低、中、高劑量組,TFN組滑膜細(xì)胞增多,凋亡率下降(P0.05);與模型組比較,HGW低、中、高劑量組,TFN組大鼠滑膜細(xì)胞凋亡率上升(P0.05);與HGW低劑量組比較,HGW高劑量組滑膜細(xì)胞凋亡率顯著上升(P0.01);與HGW-M組比較,HGW高劑量組滑膜細(xì)胞凋亡率上升(P0.05)。結(jié)論:黃芪桂枝五物湯可以促進(jìn)RA大鼠異�？哼M(jìn)的滑膜細(xì)胞凋亡。
[Abstract]:Aim: to study the effect of Huangqi Guizhi Wuwu decoction (HGW) on apoptosis of synovial cells in (CIA) rats with collagen-induced arthritis and its mechanism. Methods: Seventy-two SD rats were randomly divided into normal group, model group with low HGW, medium dose group with low HGW, high dose group with low dose of Tofacitinib (TFN), and high dose group with drug dose of 1632g kg~ (-1). The model group was given 10 mL kg~ (-1) distilled water daily. The CIA rat model was established with bovine collagen 鈪，

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