【摘要】：目的:研究黃芪桂枝五物湯(HGW)對膠原誘導型關節(jié)炎(CIA)模型大鼠關節(jié)滑膜細胞凋亡的影響,并探討其作用機制。方法:選用SD大鼠72只,隨機分為正常組,模型組,HGW低、中、高劑量組,Tofacitinib(TFN)組,HGW低、中、高劑量組藥物劑量分別為16,32,64 g·kg~(-1),相當于臨床常人用量的3,6,12倍,TFN給藥劑量為15 mg·kg~(-1),正常組、模型組每日給予10 m L·kg~(-1)蒸餾水。以牛Ⅱ型膠原加弗氏完全佐劑建立CIA大鼠模型,經HGW治療4周后光鏡下觀察各組大鼠踝關節(jié)病理形態(tài);通過流式細胞術檢測白細胞介素(IL)-4,IL~(-1)0含量,免疫組化法檢測B淋巴細胞瘤-2(B-cell lymphoma-2,Bcl-2),Bcl-2相關X蛋白(Bax)蛋白表達,DNA斷裂的原位末端標記法(TUNEL)染色檢測滑膜細胞的凋亡。結果:與正常組比較,模型組,HGW低、中、高劑量組,TFN組大鼠滑膜中Bcl-2蛋白表達增多(P0.05),模型組Bax蛋白表達減少(P0.05);與模型組比較,HGW高劑量組,TFN組大鼠滑膜中Bcl-2蛋白表達減少,Bax蛋白表達增多(P0.05)�；ぜ毎蛲隹梢�,與正常組比較,模型組,HGW低、中、高劑量組,TFN組滑膜細胞增多,凋亡率下降(P0.05);與模型組比較,HGW低、中、高劑量組,TFN組大鼠滑膜細胞凋亡率上升(P0.05);與HGW低劑量組比較,HGW高劑量組滑膜細胞凋亡率顯著上升(P0.01);與HGW-M組比較,HGW高劑量組滑膜細胞凋亡率上升(P0.05)。結論:黃芪桂枝五物湯可以促進RA大鼠異�？哼M的滑膜細胞凋亡。
[Abstract]:Aim: to study the effect of Huangqi Guizhi Wuwu decoction (HGW) on apoptosis of synovial cells in (CIA) rats with collagen-induced arthritis and its mechanism. Methods: Seventy-two SD rats were randomly divided into normal group, model group with low HGW, medium dose group with low HGW, high dose group with low dose of Tofacitinib (TFN), and high dose group with drug dose of 1632g kg~ (-1). The model group was given 10 mL kg~ (-1) distilled water daily. The CIA rat model was established with bovine collagen 鈪，

本文編號：2252247