【摘要】：實(shí)驗(yàn)背景:顱內(nèi)動(dòng)脈瘤是由于動(dòng)脈血管壁局部較脆弱、內(nèi)部壓力增高，導(dǎo)致局部血管異常而引起的腦血管瘤樣突起，發(fā)生率為0.2%一1%。由于動(dòng)脈瘤破裂的不確定性以及破裂后的高致殘率和致死率，動(dòng)脈瘤始終是神經(jīng)外科需要研究的重點(diǎn)疾病之一。為探討動(dòng)脈瘤的形態(tài)學(xué)、病理學(xué)、演變過(guò)程，我們需要建立可信可靠的動(dòng)脈瘤模型進(jìn)行血流動(dòng)力學(xué)、病理生理學(xué)以及臨床治療等多方面研究。目前在理想狀態(tài)下制作出的動(dòng)脈瘤動(dòng)物模型應(yīng)具備下列特點(diǎn):(1)無(wú)論從形態(tài)學(xué)特征或是從病理學(xué)特征都與真正的動(dòng)脈瘤相同或近似;(2)形態(tài)結(jié)構(gòu)應(yīng)相對(duì)具有穩(wěn)定性，并且應(yīng)具有較好的可重復(fù)性;(3)制作動(dòng)脈瘤模型的方法應(yīng)簡(jiǎn)便易行，操作所需時(shí)間短，所需費(fèi)用較低;(4)動(dòng)脈瘤需要有適當(dāng)?shù)捏w積，有利于實(shí)驗(yàn)觀察及進(jìn)一步操作;(5)動(dòng)脈瘤與載瘤動(dòng)脈在實(shí)驗(yàn)期間不發(fā)生血栓，動(dòng)脈瘤壁有一定強(qiáng)度，不易破裂。目前所建立的與人類形態(tài)較相似的動(dòng)脈瘤動(dòng)物模型主要有鼠、兔、犬、豬、羊和猴等動(dòng)物。因?yàn)楝F(xiàn)有的通過(guò)各種實(shí)驗(yàn)方法建立起來(lái)的任何一種動(dòng)物模型還無(wú)法完全模擬人類動(dòng)脈瘤的生物學(xué)特性，所以需要我們繼續(xù)努力研究出更加理想更加合適的動(dòng)脈瘤模型。因此，理想的動(dòng)脈瘤動(dòng)物模型的發(fā)展方向應(yīng)趨向于制作簡(jiǎn)單、可重復(fù)性高;用時(shí)短，成本低;并且血流動(dòng)力學(xué)、組織學(xué)以及病理學(xué)等與人類動(dòng)脈瘤更加接近。本實(shí)驗(yàn)應(yīng)用胰彈力蛋白酶建立兔囊狀動(dòng)脈瘤模型，并通過(guò)股動(dòng)脈造影及病理學(xué)檢查檢測(cè)模型。 實(shí)驗(yàn)材料與方法:(1)術(shù)前準(zhǔn)備:取成年新西蘭大白兔12只(體重2 .4一2.skg)，雌雄不限，分為實(shí)驗(yàn)組9只和對(duì)照組3只。麻醉應(yīng)用陸眠靈0.2 ml/1(g，戊巴比妥鈉巧mg，，kg，青霉素10萬(wàn)單位瓜g加入生理鹽水中稀釋，分別肌肉注射。(2)注入胰彈力蛋白酶:應(yīng)用外科手術(shù)解剖分離右側(cè)頸外動(dòng)脈，在頸外動(dòng)脈下方墊乳膠片并穿兩條真絲編線于其后，結(jié)扎頸外動(dòng)脈遠(yuǎn)端，頸外動(dòng)脈起始部以動(dòng)脈瘤夾臨時(shí)夾閉，用裝有生理鹽水的1毫升無(wú)菌注射器穿入盲端頸外動(dòng)脈反復(fù)沖沈后，將彈力蛋白酶20單位注入到盲端頸外動(dòng)脈內(nèi)后結(jié)扎，實(shí)驗(yàn)組在頸外動(dòng)脈盲端注入胰彈力蛋白酶，對(duì)照組在頸外動(dòng)脈注入生理鹽水。等待20分鐘后撤出動(dòng)脈臨時(shí)阻斷夾，確認(rèn)無(wú)出血后縫合皮膚。(3)術(shù)后經(jīng)股動(dòng)脈造影復(fù)查:分別于術(shù)后3天、3周行股動(dòng)脈造影監(jiān)測(cè)動(dòng)脈瘤模型。3天后復(fù)查在兔右側(cè)股動(dòng)脈搏動(dòng)最強(qiáng)處切開(kāi)皮膚，分離皮下及肌肉組織，小心分離股動(dòng)脈，遠(yuǎn)端股動(dòng)脈結(jié)扎，套管針穿刺后，置入短導(dǎo)絲，隨后置入5F鞘，推入肝素鹽水肝素化。微導(dǎo)管在微導(dǎo)絲輔助下置入主動(dòng)脈弓造影，隨后置入右側(cè)cCA，在右側(cè)頸內(nèi)動(dòng)脈開(kāi)口處推入2ml造影劑，動(dòng)脈瘤清晰可見(jiàn)。隨后給予三維旋轉(zhuǎn)造影。3周后在兔左側(cè)股動(dòng)脈行血管造影復(fù)查，方法同前。(4)病理學(xué)檢查:給予三維旋轉(zhuǎn)造影后，在靜脈注入過(guò)量戊巴比妥那處死兔子，分離頸部組織，取下動(dòng)脈瘤，置入10%EP醛溶液中固定備用。兩天后，將標(biāo)本用PBS緩沖溶液反復(fù)沖洗3次，每次沖洗五分鐘，再將標(biāo)本放入甲醛溶液中封閉一周以備用。經(jīng)過(guò)乙醇梯度脫水，包埋、修塊、半薄切片制成石蠟切片。再將石蠟切片經(jīng)HE染色后置于光鏡下觀察。 實(shí)驗(yàn)結(jié)果:(1)術(shù)后3天實(shí)驗(yàn)組9只兔子均形成動(dòng)脈瘤。動(dòng)脈瘤長(zhǎng)徑5 .0士1.20二，寬徑3.4士0.70二，載瘤動(dòng)脈直徑3_2士0.53二;術(shù)后3周動(dòng)脈瘤長(zhǎng)徑5 .12士1 .24二，寬徑346士0.70二，載瘤動(dòng)脈直徑3一2士0.52二。實(shí)驗(yàn)組動(dòng)脈瘤長(zhǎng)徑、寬徑、載瘤動(dòng)脈直徑于術(shù)后3天、3周測(cè)量的結(jié)果分別比較p值均大于0.05，各觀察項(xiàng)目在不同觀察時(shí)期均無(wú)明顯差異。對(duì)照組3只兔子分別于術(shù)后3天和3周未見(jiàn)動(dòng)脈瘤形成，血管內(nèi)造影顯示右側(cè)頸外動(dòng)脈殘端封閉。(2)實(shí)驗(yàn)組術(shù)后3周做病理學(xué)檢查發(fā)現(xiàn)H一E染色示動(dòng)脈瘤頂部新生的內(nèi)膜形成，瘤壁內(nèi)膜增生，有炎性細(xì)胞侵潤(rùn)，部分可見(jiàn)瘤壁內(nèi)膜變厚，且瘤頂部可見(jiàn)部分血栓機(jī)化。對(duì)照組術(shù)后3周H一E染色示有血栓形成并將右側(cè)頸外動(dòng)脈完全封堵，右側(cè)頸外動(dòng)脈殘端內(nèi)有血栓機(jī)化，并可見(jiàn)形成了新生的內(nèi)膜。 實(shí)驗(yàn)結(jié)論:1.采用胰彈力蛋白酶注射方法成功的建立兔囊狀動(dòng)脈瘤模型。2.通過(guò)DSA檢測(cè)方法證實(shí)建立的動(dòng)脈瘤模型在形態(tài)學(xué)上與人顱內(nèi)動(dòng)脈瘤相似。3.通過(guò)本實(shí)驗(yàn)建立的兔囊狀動(dòng)脈瘤模型成功率高，實(shí)驗(yàn)操作簡(jiǎn)便、易推廣、制作時(shí)間短、存活率高。4.通過(guò)本實(shí)驗(yàn)方法所建立的兔囊狀動(dòng)脈瘤模型形念學(xué)及病理學(xué)與人類動(dòng)脈瘤相似，故可用于血管內(nèi)介入治療、血流動(dòng)力學(xué)及發(fā)病機(jī)制方面的研究。
[Abstract]:Background: The incidence of intracranial aneurysm was 0. 2% 1%, due to the local fragility of arterial wall, the increase of internal pressure, and the occurrence of cerebral angioma-like protrusion caused by local vascular abnormality. Aneurysm is always one of the key diseases in neurosurgery, due to the uncertainty of aneurysm rupture and the post-fracture high disability rate and aneurysm rupture. To investigate the morphology, pathology and evolution of aneurysm, we need to establish a credible and reliable model of aneurysm for hemodynamic, pathophysiology and clinical treatment. An animal model of aneurysm produced under ideal conditions should be characterized by (1) the same or similar characteristics as true aneurysms, either from morphological characteristics or from pathological characteristics; (2) morphological structures should be relatively stable and should have better repeatability; (3) The method for manufacturing the aneurysm model is simple and feasible, the time required for operation is short, the required cost is lower, and (4) the aneurysm needs a proper volume, which is beneficial to experimental observation and further operation; and (5) the aneurysm and the tumor-carrying artery do not have thrombus during the experiment, The aneurysm wall has certain strength and is not easy to crack. An animal model of aneurysms similar to human form is currently established with rats, rabbits, dogs, pigs, sheep and monkeys. Because any animal model established by various experimental methods can not fully mimic the biological characteristics of human aneurysms, we need to continue our efforts to develop a more ideal and more suitable aneurysm model. Therefore, the development direction of an ideal aneurysm animal model should tend to be simple, reproducible, short in time, low in cost, and more accessible to human aneurysms, such as hemodynamics, histology, and pathology. In this experiment, the rabbit saccular aneurysm model was established by tryptic protease, and the model was detected by femoral artery angiography and pathology. Materials and Methods: (1) Pre-operative preparation: 12 adult New Zealand white rabbits (weighing 2. 4-2. skg), male and female, were divided into experimental group 9 and control group. Group 3. The anesthesia was applied to 0. 2 ml/ 1 (g, 5 mg/ kg) and 10 000 units of penicillin into normal saline for dilution, respectively. Meat injection: (2) injection of pancreatic elastic protease: apply surgical anatomy to separate right external carotid artery, cushion latex sheet under external carotid artery and wear two silk braided wires, then tie up the distal end of external carotid artery, and the initial part of external carotid artery is clamped on the aneurysm. When a sterile syringe containing physiological saline was inserted into the external carotid artery of the blind end after repeated punching, 20 units of elastic protease were injected into the external carotid artery of the blind end for ligation. The experimental group injected the pancreatic elastic protease into the blind end of the external carotid artery, and the control group was injected into the external carotid artery. Saline. After waiting for 20 minutes, withdraw the temporary occlusion clip of the artery and confirm that there is no post-bleeding seam Three days post-operative femoral artery angiography was performed to monitor the aneurysm model. After 3 days, the skin was dissected on the right femoral artery at the right side of the rabbit, subcutaneous and muscle tissue were separated, and the femoral artery and the distal femoral artery were separated carefully. Vein ligation, puncture of trocar, put into short lead wire, then put into 5F, and push into heparinized saline. heparinized. Microcatheter was inserted into aortic arch with the aid of microguidewire and then placed on the right cCA, and 2ml contrast agent was pushed into the right internal carotid artery opening, and the aneurysm was cleared. 3-dimensional rotational angiography followed by angiography review of the left femoral artery of the rabbit after 3 weeks. (4) Pathology Examination: After three-dimensional rotational angiography, rabbits were sacrificed after intravenous injection of excess pentothal, the neck tissue was separated, the aneurysm was removed, and 10% EP aldehyde solution was placed. after two days, repeatedly washing the specimen with PBS buffer solution for 3 times, washing for five minutes each time, and placing the specimen in a formaldehyde solution for one week preparing stone by using ethanol gradient dehydration, embedding, repairing block and semi-thin slice The paraffin sections were sliced. The paraffin sections were then stained with HE and placed under light microscope. The experimental results were as follows: (1) 9 rabbits in the experimental group after 3 days of operation The aneurysm was formed in the aneurysm. The diameter of the aneurysm was 5. 0 鹵 1. 20 di, the wide diameter was 3. 4 鹵 0.70II, the diameter of the tumor bearing artery was 3 _ 2 鹵 0.053 2. The diameter of the aneurysm was 5. 12 鹵 1. 24 2 in 3 weeks after the operation. The width of the aneurysm was 346 鹵 0.70II, and the diameter of the tumor-bearing artery was 3. The diameter, width and diameter of the aneurysm in the experimental group were greater than 0. 05 in the 3-week and 3-week period respectively. No significant difference was found in the period. Three rabbits in the control group were not formed in 3 days and 3 weeks after operation, and the right neck was displayed by angiography. (2) The pathological examination of 3 weeks after operation in the experimental group showed that H-E staining showed the formation of neointima in the top of the aneurysm, the intimal hyperplasia of the tumor wall, the invasion and moistening of the inflammatory cells, the thickening of the endometrial wall and the top of the tumor. Partial thrombosis was found in the control group after 3 weeks H-E staining showed thrombus formation and the right external carotid artery was completely blocked, and there was thrombosis in the left and right external carotid artery. It became the neointima. The experiment conclusions: 1. The successful construction of the method of pancreatic elastic protease injection A model of rabbit saccular aneurysm was established. The model of aneurysm established by DSA was found to be in morphology. and 3. The rabbit saccular aneurysm model established by the experiment is simple and convenient to operate, easy to popularize and prepare. Conclusion The model shape and pathology of rabbit saccular aneurysm established by this method are similar to that of human aneurysm, so it can be used for endovascular interventional therapy and hemodynamics.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R-332

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 薛德麟;;顱內(nèi)動(dòng)脈瘤的手術(shù)經(jīng)驗(yàn)[J];華中醫(yī)學(xué)雜志;1986年03期

2 翟秀偉;孫希明;董京林;杜金;白海欣;王大勇;李孝民;張宇;;顱內(nèi)動(dòng)脈瘤的顯微手術(shù)治療[J];醫(yī)學(xué)研究雜志;2003年02期

3 戰(zhàn)華;劉相軫;韓占強(qiáng);史懷璋;陳會(huì)榮;孫志丹;;翼點(diǎn)入路治療基底動(dòng)脈上段破裂大動(dòng)脈瘤一例[J];中國(guó)腦血管病雜志;2006年11期

4 周志敏;羊正祥;耿炯;陳翔;繆偉鋒;;三維CT血管造影在顱內(nèi)動(dòng)脈瘤急診手術(shù)中的初步應(yīng)用[J];山西醫(yī)藥雜志;2007年06期

5 張凌;李玉雄;梁鑫;郭子運(yùn);張雄新;;巨大寬基底大腦中動(dòng)脈內(nèi)側(cè)生長(zhǎng)動(dòng)脈瘤夾閉術(shù)1例[J];中國(guó)實(shí)用醫(yī)藥;2008年06期

6 劉婉云;;前交通動(dòng)脈瘤夾閉術(shù)的手術(shù)配合[J];內(nèi)蒙古中醫(yī)藥;2008年17期

7 李驍雄;加藤庸子;佐野公俊;;神經(jīng)內(nèi)窺鏡在動(dòng)脈瘤顯微手術(shù)中的應(yīng)用[J];國(guó)際神經(jīng)病學(xué)神經(jīng)外科學(xué)雜志;2006年05期

8 隋強(qiáng)波;丁璇;王成偉;王志剛;;大腦中動(dòng)脈動(dòng)脈瘤手術(shù)探討[J];實(shí)用醫(yī)學(xué)雜志;2008年17期

9 王義寶;景治濤;劉云會(huì);王運(yùn)杰;;顱內(nèi)后循環(huán)動(dòng)脈瘤的診斷與治療(附23例病例分析)[J];中國(guó)醫(yī)科大學(xué)學(xué)報(bào);2009年05期

10 覃家德;黃瑋;余永佳;劉愛(ài)華;廖振南;張濟(jì)源;;大腦中動(dòng)脈遠(yuǎn)端動(dòng)脈瘤破裂1例報(bào)告并文獻(xiàn)復(fù)習(xí)[J];中國(guó)臨床神經(jīng)外科雜志;2010年11期

相關(guān)會(huì)議論文 前10條

1 曾而明;;床突旁動(dòng)脈瘤的外科治療策略[A];中華醫(yī)學(xué)會(huì)神經(jīng)外科學(xué)分會(huì)第九次學(xué)術(shù)會(huì)議論文匯編[C];2010年

2 孫克華;盧亦成;;腦動(dòng)脈瘤切除術(shù)在臨床的應(yīng)用價(jià)值(附39例報(bào)告)[A];中國(guó)醫(yī)師協(xié)會(huì)神經(jīng)外科醫(yī)師分會(huì)第四屆全國(guó)代表大會(huì)論文匯編[C];2009年

3 尹連虎;呂新兵;孫超;呂建華;王長(zhǎng)江;王浩;王剛;張紅兵;田力學(xué);;自發(fā)性破裂出血的顱內(nèi)動(dòng)脈瘤的治療體會(huì)[A];中國(guó)醫(yī)師協(xié)會(huì)神經(jīng)外科醫(yī)師分會(huì)第四屆全國(guó)代表大會(huì)論文匯編[C];2009年

4 褚正民;周海航;金成勝;沈建國(guó);王翊飛;王耿煥;褚聞來(lái);張李濤;;自發(fā)性蛛網(wǎng)膜下腔出血的診治[A];2011年浙江省神經(jīng)外科學(xué)學(xué)術(shù)年會(huì)論文匯編[C];2011年

5 李貞偉;姚鑫;楊玉山;陳步東;;眼動(dòng)脈段動(dòng)脈瘤的手術(shù)治療[A];中華醫(yī)學(xué)會(huì)神經(jīng)外科學(xué)分會(huì)第九次學(xué)術(shù)會(huì)議論文匯編[C];2010年

6 王保平;葉錦平;張翼;梅yN民;林偉;麻偉興;;小骨窗與傳統(tǒng)骨瓣開(kāi)顱在動(dòng)脈瘤手術(shù)應(yīng)用中的利弊分析附86例小骨窗手術(shù)報(bào)道[A];2009年浙江省神經(jīng)外科學(xué)術(shù)年會(huì)論文匯編[C];2009年

7 張建民;陳高;沈宏;傅偉明;祝向東;胡華;吳群;張宏;王林;閆偉;;動(dòng)脈瘤性蛛網(wǎng)膜下腔出血的早期診治[A];2009年浙江省神經(jīng)外科學(xué)術(shù)年會(huì)論文匯編[C];2009年

8 呂發(fā)金;謝鵬;羅天友;張志偉;方維東;何怡紅;;數(shù)字減影CTA評(píng)價(jià)動(dòng)脈瘤夾閉術(shù)后改變的初步研究[A];中華醫(yī)學(xué)會(huì)第十三屆全國(guó)放射學(xué)大會(huì)論文匯編（下冊(cè)）[C];2006年

9 杭春華;史繼新;王漢東;謝偉;潘云曦;成惠林;樊有武;孫康建;;大腦中動(dòng)脈動(dòng)脈瘤的臨床特點(diǎn)及外科治療[A];中國(guó)醫(yī)師協(xié)會(huì)神經(jīng)外科醫(yī)師分會(huì)第二屆全國(guó)代表大會(huì)論文匯編[C];2007年

10 郭建;孟祥靖;孫金龍;魏麟;孔建新;劉廣存;;兩例顱內(nèi)微小動(dòng)脈瘤治療分析[A];中華醫(yī)學(xué)會(huì)神經(jīng)外科學(xué)分會(huì)第九次學(xué)術(shù)會(huì)議論文匯編[C];2010年

相關(guān)重要報(bào)紙文章 前10條

1 羅學(xué)宏　(中南大學(xué)湘雅醫(yī)院急診科 教授);警惕腦內(nèi)“定時(shí)炸彈”[N];醫(yī)藥經(jīng)濟(jì)報(bào);2011年

2 牛斯;治顱動(dòng)脈瘤效果佳[N];醫(yī)藥經(jīng)濟(jì)報(bào);2002年

3 記者 邢飛;神經(jīng)外科禁區(qū)的“挑戰(zhàn)者”[N];撫順日?qǐng)?bào);2008年

4 凌鋒;高山仰止[N];健康報(bào);2003年

5 丁殿春 劉泉 本報(bào)特約通訊員　 黃余紅;守護(hù)人體大腦的安全[N];解放軍報(bào);2006年

6 黃余紅 丁殿春 劉泉;大腦的“保護(hù)神”[N];中國(guó)醫(yī)藥報(bào);2006年

7 孟懷東;危及生命的頭痛[N];中國(guó)中醫(yī)藥報(bào);2003年

8 通訊員 艾賽提·阿木提;大愛(ài)點(diǎn)燃生命之光[N];和田日?qǐng)?bào)（漢）;2010年

9 陸書(shū)鑫　張磊 張茂春;紅興隆黨建模范區(qū)為跨越注入新活力[N];北大荒日?qǐng)?bào);2010年

10 陳漢橋;發(fā)現(xiàn)一個(gè)“間隙” 突破一個(gè)“禁區(qū)”[N];中國(guó)醫(yī)藥報(bào);2003年

相關(guān)博士學(xué)位論文 前10條

1 李琦;腦血管疾病的血管影像學(xué)研究[D];重慶醫(yī)科大學(xué);2011年

2 符洋;貝納普利抑制家兔腎下腹主動(dòng)脈瘤形成的研究[D];中南大學(xué);2009年

3 馬朝暉;中西結(jié)合治療動(dòng)脈瘤性蛛網(wǎng)膜下腔出血的臨床研究[D];廣州中醫(yī)藥大學(xué);2010年

4 周迎春;局部應(yīng)用醫(yī)用生物蛋白膠緩釋神經(jīng)生長(zhǎng)因子對(duì)損傷視神經(jīng)和視網(wǎng)膜節(jié)細(xì)胞保護(hù)作用的實(shí)驗(yàn)研究[D];華中科技大學(xué);2005年

5 宋潤(rùn)蘭;實(shí)驗(yàn)性金黃地鼠肺氣腫相關(guān)因素的探討及中藥防治觀察[D];中國(guó)協(xié)和醫(yī)科大學(xué);1998年

6 張文清;犬嫁接性梭形動(dòng)脈瘤轉(zhuǎn)移性生物膜包裹治療的實(shí)驗(yàn)研究[D];南方醫(yī)科大學(xué);2007年

7 佟丹;多層螺旋計(jì)算機(jī)斷層血管造影在國(guó)人破裂的顱內(nèi)動(dòng)脈瘤的研究[D];吉林大學(xué);2010年

8 廖旭興;顱內(nèi)多發(fā)動(dòng)脈瘤發(fā)病機(jī)制、新手術(shù)入路顯微解剖及應(yīng)用研究[D];南方醫(yī)科大學(xué);2010年

9 汪浩;強(qiáng)力霉素抑制大鼠腹主動(dòng)脈瘤形成的實(shí)驗(yàn)性研究[D];第二軍醫(yī)大學(xué);2002年

10 張曉冬;不同手術(shù)方式對(duì)顱內(nèi)動(dòng)脈瘤血管痙攣及預(yù)后的影響[D];重慶醫(yī)科大學(xué);2011年

相關(guān)碩士學(xué)位論文 前10條

1 馬馳;一種新型彈力蛋白酶誘導(dǎo)兔動(dòng)脈瘤模型的建立[D];吉林大學(xué);2011年

2 費(fèi)邵陽(yáng);兔動(dòng)脈瘤模型模擬不全夾閉前后血液動(dòng)力學(xué)改變的對(duì)比研究[D];吉林大學(xué);2010年

3 許政;實(shí)驗(yàn)性兔腦動(dòng)脈瘤模型的建立及NF-κB、MCP-1和MMP-9在模型中的表達(dá)及機(jī)制探討[D];蘇州大學(xué);2010年

4 張路陽(yáng);三維CT血管造影在前交通支動(dòng)脈瘤診治中的應(yīng)用[D];中國(guó)醫(yī)科大學(xué);2010年

5 韓超;椎基底動(dòng)脈動(dòng)脈瘤治療策略的臨床研究[D];山東大學(xué);2011年

6 黃乾亮;彈性蛋白酶誘導(dǎo)兔囊性動(dòng)脈瘤模型的建立及其病理學(xué)研究[D];南昌大學(xué);2009年

7 蔡敬;兔實(shí)驗(yàn)性頸動(dòng)脈瘤模型的建立及MMP-1、MMP-2、MMP-9、TIMP-2在模型中的表達(dá)研究[D];四川大學(xué);2005年

8 吳賢群;動(dòng)脈瘤性蛛網(wǎng)膜下腔出血患者顯微外科手術(shù)后預(yù)后的影響因素分析[D];中南大學(xué);2009年

9 陳敬寅;血管內(nèi)介入治療在小腦后下動(dòng)脈動(dòng)脈瘤治療中的應(yīng)用[D];浙江大學(xué);2012年

10 劉海玉;大腦中動(dòng)脈分叉處動(dòng)脈瘤及其不完全夾閉的血流動(dòng)力學(xué)分析[D];吉林大學(xué);2011年

本文編號(hào)：2252111