發(fā)布時(shí)間：2018-09-19 20:02

【摘要】：目的構(gòu)建臨床代表性好、穩(wěn)定性佳的艱難梭菌感染小鼠模型,為艱難梭菌感染相關(guān)疾病提供研究工具。方法選擇C57BL/6、BALB/c、昆明小鼠,經(jīng)抗生素誘導(dǎo)后,分別予以不同濃度(108 CFU/mL~10~(10) CFU/mL)的臨床分離菌株混懸液灌胃,觀察不同品系小鼠不同時(shí)間點(diǎn)腹瀉、全身情況及結(jié)腸組織病理學(xué)變化。結(jié)果灌菌后,BALB/c小鼠10~(10) CFU/mL濃度組全部出現(xiàn)腹瀉,死亡率為16.7%;其他實(shí)驗(yàn)組小鼠腹瀉程度差異較大或僅部分出現(xiàn)輕度腹瀉。腹瀉小鼠表現(xiàn)為稀爛便甚至水樣便和濕尾現(xiàn)象,體重減輕,腸道病理顯示結(jié)腸黏膜充血水腫伴炎性細(xì)胞浸潤。結(jié)論經(jīng)5種抗生素灌胃9d+克林霉素腹腔注射誘導(dǎo),10~(10) CFU/mL艱難梭菌活菌混懸液灌胃后構(gòu)建的BALB/c小鼠艱難梭菌感染模型最穩(wěn)定。
[Abstract]:Objective to construct a mouse model of Clostridium difficulty infection with good clinical representation and stability, and to provide a tool for the study of Clostridium diffracta infection related diseases. Methods C57BL / 6 BALB / cand Kunming mice were induced by antibiotics, and then were perfused with different concentrations (108 CFU/mL~10~ (10) CFU/mL) of clinical isolated strains to observe the diarrhea, systemic conditions and colonic histopathological changes at different time points in different strains of mice. Results diarrhea was found in all the 10 ~ (10) CFU/mL groups of BALB / c mice, and the mortality was 16.70.The diarrhea degree in other experimental groups was higher than that in other experimental groups. Diarrhea mice showed dilute stool, even watery stool and wet tail, weight loss, intestinal pathology showed that colonic mucosa hyperemia and edema with inflammatory cell infiltration. Conclusion the model of Clostridium difficulty infection in BALB/c mice was established by intraperitoneal injection of clindamycin for 9 days after 5 antibiotics were perfused to induce 10 ~ (10) CFU/mL Clostridium difficult live bacteria suspension.
【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院消化內(nèi)科;
【基金】：國家自然科學(xué)基金(8157041627) 國家“863”計(jì)劃專題(2015AA020701)
【分類號(hào)】：R-332;R574.62

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