【摘要】：目的探討人特異的胰島素放射免疫分析試劑盒(Ins-RIA)檢測小型豬血清胰島素水平的可行性。方法采集219份巴馬小型豬血清標(biāo)本,分別采用人Ins-RIA和豬特異的胰島素ELISA試劑盒(Ins-ELISA)測定血清胰島素水平。分析兩種方法檢測豬胰島素水平的相關(guān)性,隨機(jī)抽取80%(175份)的數(shù)據(jù)進(jìn)行直線回歸分析,以Ins-ELISA為因變量,Ins-RIA為自變量,建立回歸方程,再用剩余20%(44份)的數(shù)據(jù)對回歸方程進(jìn)行回代驗(yàn)證。結(jié)果在巴馬小型豬,豬Ins-ELISA檢測值低于人Ins-RIA檢測值,分別為(15.32±15.50)μIU/mL及(32.31±21.74)μIU/mL,差異有統(tǒng)計(jì)學(xué)意義(P0.000 1);預(yù)測豬Ins-ELISA檢測胰島素的回歸方程為InsELISA(μIU/mL)=-7.29+0.70×Ins-RIA(μIU/mL)(R~2=0.94);在回代驗(yàn)證中,Ins-ELISA的預(yù)測值和實(shí)際檢測值之間的平均差異為17.18%,預(yù)測值與實(shí)際測量值之間的吻合程度較好(P0.05)。結(jié)論巴馬小型豬ELISA法檢測的血清胰島素水平可以用人胰島素RIA法檢測的血清胰島素水平來進(jìn)行估計(jì)。
[Abstract]:Objective to investigate the feasibility of human specific insulin radioimmunoassay kit (Ins-RIA) for detection of serum insulin in miniature pigs. Methods Human Ins-RIA and porcine specific insulin ELISA kit (Ins-ELISA) were used to detect serum insulin level in 219 Bama miniature pig serum samples. By analyzing the correlation between the two methods to detect the insulin level in pigs, 80% (175 samples) of the data were randomly sampled for linear regression analysis. The regression equation was established with Ins-ELISA as the dependent variable and Ins-RIA as the independent variable. The regression equation was verified with the data of the remaining 20% (44 copies). Results in Bama mini-pigs, the detection value of pig Ins-ELISA was lower than that of human Ins-RIA. There were significant differences between (15.32 鹵15.50) 渭 IU/mL and (32.31 鹵21.74) 渭 IU/mL, respectively (P0.0001), the regression equation of predicting insulin detection by Ins-ELISA was InsELISA (渭 IU/mL) -7.29 0.70 脳 Ins-RIA (渭 IU/mL) (RP2O0.94), and the average difference between the predicted value of Ins-ELISA and the actual value of detection was 17.18% in the back generation verification, and the regression equation was InsELISA (渭 IU/mL) -7.29 0.70 脳 Ins-RIA (渭 IU/mL), and the average difference between the predicted value and the actual measured value was 17.18. The degree of anastomosis was good (P0.05). Conclusion the serum insulin level measured by ELISA method in Bama miniature pigs can be estimated by the serum insulin level measured by human insulin RIA method.
【作者單位】： 四川大學(xué)華西醫(yī)院內(nèi)分泌代謝科糖尿病足診治中心;成都達(dá)碩實(shí)驗(yàn)動物有限公司;
【基金】：國家自然科學(xué)基金(No.81170776,No.81471043)資助
【分類號】：R392-33

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