【摘要】：目的目前有關(guān)家兔的胰腺癌模型制作方法報(bào)道較為少見,文中比較Panc-1細(xì)胞懸液原位種植與VX-2組織懸液原位種植在家兔胰腺癌模型建立中的效果。方法選取健康家兔30只,采取隨機(jī)數(shù)字表法分為組織懸液組(n=15)、細(xì)胞懸液組(n=15)。組織懸液組采取VX-2組織懸液原位種植,細(xì)胞懸液組采取Panc-1細(xì)胞懸液原位種植,種植后觀察模型的建立情況。通過B超、3.0T磁共振以及CT等輔助檢查評(píng)價(jià)兩種建模結(jié)果。結(jié)果組織懸液組第3周內(nèi)有5只家兔出現(xiàn)十二指腸、結(jié)腸、盲腸、腹膜壁出現(xiàn)大量腫瘤組織種植性轉(zhuǎn)移,3只家兔大網(wǎng)膜出現(xiàn)腫瘤組織種植性轉(zhuǎn)移,MR T2見胃體后高信號(hào)影。細(xì)胞懸液組第3周內(nèi)1只家兔十二指腸出現(xiàn)腫瘤組織種植性轉(zhuǎn)移,MR LAVA見胃體后方稍高信號(hào)影。組織懸液組中15只模型全部建立成功,細(xì)胞懸液組僅發(fā)現(xiàn)3例建模成功。與組織懸液組比較,細(xì)胞懸液組第3、4周腫瘤種植成功率明顯降低(46.66%vs 6.67%,100%vs 20.00%,P0.05)。結(jié)論 VX-2組織懸液原位種植較Panc-1細(xì)胞懸液原位種植的更具有可行性,更易實(shí)施。
[Abstract]:Objective to compare the effect of in situ implantation of Panc-1 cell suspension and VX-2 tissue suspension on the establishment of rabbit pancreatic carcinoma model. Methods 30 healthy rabbits were randomly divided into tissue suspension group (n = 15) and cell suspension group (n = 15). VX-2 tissue suspension was implanted in situ in the tissue suspension group and Panc-1 cell suspension was implanted in the cell suspension group. The establishment of the model was observed after implantation. The modeling results were evaluated by 3. 0T magnetic resonance imaging (MRI) and computed tomography (CT). Results in the third week of tissue suspension group, there were 5 rabbits with duodenum, colon, cecum and peritoneal wall with a large number of implanted metastasis. 3 rabbits had tumor tissue implantation metastasis in the greater omentum. Mr T2 showed high signal intensity behind the gastric body. In the third week of the cell suspension group, a rabbit duodenum showed implanted metastasis of tumor tissue. Mr LAVA showed a little hyperintense image behind the gastric body. In the tissue suspension group, all the 15 models were successfully established, and only 3 cases were successfully established in the cell suspension group. Compared with the tissue suspension group, the success rate of tumor implantation in the cell suspension group was significantly lower than that in the cell suspension group at the 3rd week (46.66%vs 6.67 vs 20.00% P0.05). Conclusion in situ implantation of VX-2 tissue suspension is more feasible and easier than that of Panc-1 cell suspension in situ implantation.
【作者單位】： 齊齊哈爾醫(yī)學(xué)院醫(yī)學(xué)技術(shù)學(xué)院;齊齊哈爾醫(yī)學(xué)院附屬第三醫(yī)院MR室;
【基金】：黑龍江省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃重點(diǎn)項(xiàng)目(201611230004)
【分類號(hào)】：R735.9;R-332

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