【摘要】：目的研究黃芩苷對注意缺陷多動障礙(ADHD)模型大鼠行為學特征的影響,為黃芩苷治療ADHD的進一步研究提供依據(jù)。方法將40只SHR大鼠隨機分為模型組、鹽酸哌甲酯組及黃芩苷低、中、高劑量組,每組8只,另設8只WKY大鼠為正常對照組。鹽酸哌甲酯組(0.07 mg/mL)及黃芩苷低劑量(3.33 mg/mL)、中劑量(6.67 mg/mL)、高劑量(10 mg/mL)組分別按體重(1.5 mL/100 g)給予對應藥物灌胃,正常對照組、模型組給予等量生理鹽水灌胃,各組大鼠灌胃4周,每日2次。曠場實驗觀察實驗第0天及灌胃后7、14、21、28 d各組大鼠的總運動距離及平均運動速度,評估藥物對大鼠多動、沖動行為的控制作用;Morris水迷宮實驗觀察各組大鼠的潛伏期、目標象限活動比率及穿越平臺次數(shù),評估藥物對大鼠注意力的影響。結(jié)果曠場實驗數(shù)據(jù)顯示,第0天,模型組及各用藥組大鼠的總運動距離及平均運動速度均顯著高于正常對照組(P0.05);第7天,鹽酸哌甲酯組總運動距離及平均運動速度較模型組顯著降低(P0.05);第14天,鹽酸哌甲酯組及黃芩苷高劑量組大鼠在總運動距離及平均運動速度上均較模型組顯著降低(P0.05);第21天及第28天,黃芩苷各劑量組在總運動距離及平均運動速度上較模型組均呈不斷下降趨勢(P0.05)。水迷宮空間探索實驗結(jié)果顯示,鹽酸哌甲酯組及黃芩苷中、高劑量組目標象限停留時間比率顯著高于模型組(P0.05),鹽酸哌甲酯組、黃芩苷高劑量組目標象限運動距離所占比率亦顯著高于模型組(P0.05);黃芩苷高劑量組穿越平臺次數(shù)高于其余各組(P0.05)。結(jié)論黃芩苷及鹽酸哌甲酯均能夠調(diào)控ADHD模型SHR大鼠的運動能力及學習記憶能力,從而控制ADHD多動、沖動及注意力不集中的核心癥狀;黃芩苷藥效呈劑量依賴性,高劑量黃芩苷療效最為顯著,但其藥效的發(fā)揮遲于鹽酸哌甲酯。
[Abstract]:Objective to study the effect of Baicalin on the behavioral characteristics of the model rats with attention deficit hyperactivity disorder (ADHD), and to provide a basis for further study of Baicalin in the treatment of ADHD. Methods 40 SHR rats were randomly divided into model group, methylphenidate group and Baicalin in low, middle, high dose group, 8 rats in each group, and 8 WKY rats were normal control group. The methyl group (0.07 mg/mL) and baicalin were low dose (3.33 mg/mL), middle dose (6.67 mg/mL), and high dose (10 mg/mL) group were given the corresponding medicine according to body weight (1.5 mL/100 g), and the normal control group was given the normal control group. The model group was given the same amount of normal saline for 4 weeks, 2 times a day. The open field experiment was observed for zeroth days and 7,14,21,28 d after gavage. The total movement distance and average speed of the rats in each group were used to evaluate the effect of drugs on the hyperactivity and impulse behavior of rats. The Morris water maze test observed the latency of the rats, the ratio of target quadrants and the number of crossing platforms, and evaluated the effect of the drug on the attention of the rats. The results of the open field experiment showed that the model group and the model group were zeroth days. The total movement distance and average motor speed of the rats in each group were significantly higher than that of the normal control group (P0.05). The total movement distance and average velocity of the methyl piperidine hydrochloride group were significantly lower than those of the model group (P0.05) on the seventh day. The fourteenth day, the total movement distance and the average movement speed of the high dose group of methylphenidate and baicalin were compared with the model rats. In the twenty-first and 28 days, the total movement distance and average velocity of Baicalin in the twenty-first days and the 28 days were lower than those in the model group (P0.05). The results of the water maze space exploration experiment showed that the ratio of the target residence time of the high dose group was significantly higher than that of the model group (P0.05). The ratio of target quadrant distance of high dose baicalin group was significantly higher than that of model group (P0.05), and the number of high dose group of baicalin high dose group was higher than that of other groups (P0.05). Conclusion baicalin and methylphenidate can control the exercise ability and learning and memory ability of ADHD model SHR rats, thus controlling ADHD hyperactivity. The core symptoms of impulsiveness and concentration were not concentrated; the efficacy of baicalin was dose-dependent and the effect of high dose baicalin was most significant, but its efficacy was later than methylphenidate.
【作者單位】： 南京中醫(yī)藥大學中醫(yī)兒科研究所/江蘇省兒童呼吸疾病(中醫(yī)藥)重點實驗室;
【基金】：江蘇省教育廳研究生創(chuàng)新工程項目(KYZZ16_0410) 國家自然科學基金(81273801)
【分類號】：R285.5;R-332

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