本文選題：寒凝血瘀型 + 消化性潰瘍　； 參考：《遼寧中醫(yī)藥大學》2011年碩士論文

【摘要】：目的:建立寒凝血瘀型消化性潰瘍大鼠模型,為臨床研究提供實驗模型。 材料與方法: 20只大鼠隨機分為2組,模型組(10只),采用改良冷暴露法,冷暴露同時,給予吲哚美辛,造成寒凝血瘀型消化性潰瘍模型,對照組(10只),不施加冷暴露處理,肉眼及光鏡觀察大鼠癥狀、體征及胃粘膜潰瘍情況,同時檢測胃粘膜微循環(huán)血流量及血流變學改變。 結(jié)果: 模型組大鼠冷凍后出現(xiàn)蜷縮少動、反應遲鈍、寒戰(zhàn),喜扎堆,呼吸微弱,被毛無光澤,飲水量少,小便色清,大便濕爛,爪尾部紫暗,耳色暗紅等癥狀,光鏡下可見胃黏膜有明顯損傷,條索狀糜爛,以胃體部為主,整個黏膜層細胞有明顯變性、胃小凹消失;主細胞、粘液細胞腫大,壞死或脫落,固有膜有灶性出血及急性炎癥反應,損傷穿透黏膜層。對照組大鼠精神良好,動作敏捷有力,攝食正常,被毛密澤,緊貼身體,體肌健壯,胃黏膜基本正常。模型組胃粘膜潰瘍指數(shù)(11.55±2.74)明顯高于對照組(P0.01)。模型組血液流變學指標亦較對照組升高(P0.01)。模型組胃粘膜微循環(huán)血流量(57.18±7.12)明顯少于對照組(87.06±3.40)(P0.01)。 結(jié)論: 采用改良冷暴露聯(lián)合吲哚美辛法建立的寒凝血瘀型消化性潰瘍大鼠模型,符合中醫(yī)證侯及消化性潰瘍“病癥結(jié)合”的特點,適合中醫(yī)學實驗研究。
[Abstract]:Objective: to establish a rat model of peptic ulcer with cold coagulation and blood stasis, and to provide an experimental model for clinical study. Materials and methods: twenty rats were randomly divided into two groups: the model group (n = 10) and the control group (n = 10). The symptoms, signs and gastric mucosal ulcers were observed with naked eye and light microscope. The blood flow and hemorheology of gastric mucosal microcirculation were also detected. Results: in the model group, the rats were curled up and moved, slow in reaction, shivering, faint in breathing, dull in hair, less in drinking water, clear in urine, wet in stool, purple in the tail of claw, dark red in ear, and so on. Under the light microscope, the gastric mucosa was obviously damaged, striped erosion, mainly in the body of the stomach, the cells of the whole mucosal layer were obviously denatured, the fovea of the stomach disappeared, the main cells, the mucous cells were enlarged, necrotized or shedded, The lamina propria has focal hemorrhage and acute inflammatory reaction, which damages and penetrates the mucosal layer. The rats in the control group were in good spirits, quick and strong, normal food intake, Mao Mizawa, close to the body, strong body muscles and normal gastric mucosa. The gastric mucosal ulcer index in the model group (11.55 鹵2.74) was significantly higher than that in the control group (P0.01). The hemorheological indexes in the model group were also higher than those in the control group (P0.01). The blood flow of gastric mucosal microcirculation in the model group (57.18 鹵7.12) was significantly lower than that in the control group (87.06 鹵3.40) (P0.01). Conclusion: the model of peptic ulcer of cold coagulation and blood stasis type was established by modified cold exposure combined with indomethacin method, which accords with the characteristics of TCM syndromes and peptic ulcer "disease combination", and is suitable for the experimental study of traditional Chinese medicine.
【學位授予單位】：遼寧中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R-332

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