本文選題：下腔靜脈 + 動物模型��； 參考：《重慶醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：建立VX2腫瘤侵犯下腔靜脈（IVC）的動物模型，并觀察相應(yīng)的生物學(xué)行為。 方法：經(jīng)腹直視下，將VX2腫瘤組織塊分別種植在28只兔腎以下IVC附近的軟組織內(nèi)，然后隨機(jī)分為A、B兩組。A組術(shù)后每周在超聲下觀察腫瘤的大小，IVC和腹主動脈（AA）管徑大小，對周圍組織的侵犯情況；DSA觀察側(cè)支循環(huán)建立。B組每周隨機(jī)解剖4只兔子，取IVC和AA做病理和免疫組化（PCNA）驗查，并觀察轉(zhuǎn)移情況。 結(jié)果： 1，超聲顯示：所有腫瘤均種植成功，腫瘤面積大小與生長時間呈明顯正相關(guān)（r1=0.879），IVC管徑與腫瘤面積大小顯著負(fù)相關(guān)（r2=-0.8295）；4周時25%的IVC管腔閉塞，5周時達(dá)到91.7%； 2，，病理和免疫組化顯示：IVC管壁被腫瘤細(xì)胞取代；觀察期內(nèi)腫瘤未突破AA管壁外膜。 3，DSA顯示：IVC慢性受侵犯過程中側(cè)枝循環(huán)建立良好。 4，解剖觀察：模型動物在第4周開始出現(xiàn)肝、肺和腹腔轉(zhuǎn)移。 結(jié)論：將VX2組織塊種植于IVC內(nèi)側(cè)可得到模擬人體惡性腫瘤侵犯IVC的理想動物模型。
[Abstract]:Objective: to establish an animal model of VX 2 tumor invading inferior vena cava (IVC) and observe its biological behavior. Methods: VX2 tumor tissue was implanted into the soft tissue near IVC in 28 rabbits, and then divided into two groups randomly. Group A was randomly divided into two groups: group A, the size of the tumor and the diameter of the abdominal aorta (AA) were observed under ultrasound every week after operation. The collateral circulation was observed by DSA. In group B, 4 rabbits were dissected randomly every week. IVC and AA were taken for pathological and immunohistochemical examination, and metastasis was observed. Results: (1) Ultrasonography showed that all the tumors were successfully implanted, and the size of the tumor was positively correlated with the growth time (r1n0.879). The diameter of IVC was negatively correlated with the size of the tumor area (r _ 2 ~ (-0.8295). At 4 weeks, 25% of the IVC lumen occlusion reached 91.7% at 5 weeks. (2) pathological and immunohistochemical findings showed that the wall of the IVC was replaced by tumor cells. During the observation period, the tumor did not break through the adventitia of AA wall. 3 DSA showed that the collateral circulation was well established during the chronic invasion of VIVC. 4. Anatomical observation: liver, lung and abdominal cavity metastases began to appear in the model animal at the 4th week. Conclusion: the ideal animal model of human malignant tumor invading IVC can be obtained by planting VX2 tissue on the medial side of IVC.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R73-36;R-332

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