本文選題：充氣止血帶 + 肢體　； 參考：《新疆醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：用充氣止血帶制作肢體缺血再灌注損傷的新型動(dòng)物模型，研究其對(duì)周圍神經(jīng)和骨骼肌損傷的影響。方法：選擇健康新西蘭大白兔6月齡，30只，體重3.5±0.3Kg，雌雄不限，隨機(jī)分為3組，每組10只：A組：對(duì)照組，B組：缺血2小時(shí)，C組：缺血4小時(shí)。在家兔左側(cè)后肢環(huán)扎充氣止血帶，于不同時(shí)間點(diǎn)松開，造成左側(cè)后肢缺血再灌注損傷的模型。對(duì)照組不扎充氣止血帶，于第1、2、3、4、5、6小時(shí)檢測(cè)肢體的神經(jīng)電生理學(xué)，B組、C組于再灌注（松開止血帶，血供恢復(fù)后）的1、2、3、4、5、6小時(shí)檢測(cè)肢體的神經(jīng)電生理學(xué)，A組于第6小時(shí)觀察骨骼肌的形態(tài)學(xué)，B組、C組于再灌注（松開止血帶，血供恢復(fù)后）的第6小時(shí)觀察骨骼肌的形態(tài)學(xué)，每組于術(shù)后第5天觀察、評(píng)估左側(cè)后肢的行走功能。結(jié)果：隨著缺血時(shí)間的延長(zhǎng)、再灌注的損傷，B組、C組和A組相比較，周圍神經(jīng)的潛伏期延長(zhǎng)、波幅降低，，傳導(dǎo)速度降低，三組之間的潛伏期、波幅、傳導(dǎo)速度差異均有統(tǒng)計(jì)學(xué)意義（P＜0.05），光鏡觀察骨骼肌可見（B組和C組）：橫紋紊亂、肌纖維斷裂、間質(zhì)血管擴(kuò)張充血、大量中性粒細(xì)胞浸潤(rùn)。結(jié)論：經(jīng)過缺血期和再灌注損傷的交互作用后，肢體的功能性損傷進(jìn)一步加重，出現(xiàn)了不可逆的病損。該模型制作對(duì)動(dòng)物的損傷較小，更貼近臨床。
[Abstract]:Aim: to study the effects of inflatable tourniquet on peripheral nerve and skeletal muscle injury. Methods: 30 healthy New Zealand white rabbits were randomly divided into 3 groups (n = 10): control group (n = 2): group C: ischemia (n = 4). The inflatable tourniquet was ligated on the left hind limb of the rabbit and released at different time points to make the model of ischemia reperfusion injury of the left hind limb. The control group did not tie an inflatable tourniquet, and the limbs were examined for neuroelectrophysiology at 6 hours at 1, 2, 3, 4, 5 and 6 hours after reperfusion (loosening the tourniquet) in group B and C, respectively. 6 hours after recovery of blood supply, the morphology of skeletal muscle was observed in group A at 6 hours and the morphology of skeletal muscle in group C at 6 hours after reperfusion (release of tourniquet and recovery of blood supply). The walking function of left hindlimb was evaluated 5 days after operation in each group. Results: with the prolongation of ischemic time, compared with group A, the latency, amplitude and conduction velocity of peripheral nerve in group B were prolonged, and the latency and amplitude of wave were decreased between the three groups. The difference of conduction velocity was statistically significant (P < 0.05). The skeletal muscle was observed under light microscope (group B and C): transverse striation, broken muscle fiber, hyperemia of interstitial vessels and infiltration of neutrophils. Conclusion: after the interaction between ischemia and reperfusion injury, the functional injury of limbs is further aggravated and irreversible lesions appear. The model has less damage to animals and is closer to clinical practice.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R658;R-332

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