本文選題：家兔 + 烏司他丁　； 參考：《南華大學(xué)》2012年碩士論文

【摘要】：目的 將脂多糖(lipopolysaccharide, LPS)注入輸尿管內(nèi)并結(jié)扎該側(cè)輸尿管建立尿源性膿毒血癥的兔模型；探討烏司他丁對膿毒血癥的療效,為烏司他丁治療尿源性膿毒血癥提供理論和實(shí)際依據(jù)。 方法 1、選擇結(jié)扎家兔左側(cè)輸尿管,左側(cè)輸尿管內(nèi)注入脂多糖,建立尿路梗阻及泌尿系感染并膿毒癥的動物模型。 2、將24只健康雄性家兔隨機(jī)分成四組,每組6只。正常組(Xormal組),假手術(shù)組(Sham組),模型組(Model組)和治療組(UTI組)。 3、造模36h后監(jiān)測各組實(shí)驗(yàn)家兔體溫,呼吸頻率,檢測外周血細(xì)胞計(jì)數(shù)、血清C反應(yīng)蛋白；采用ELISA法測定血清TNF-α、IL-10的含量；各組肝、腎、肺組織行HE染色,光學(xué)顯微鏡下觀察各組織的形態(tài)結(jié)構(gòu)變化；免疫組織化學(xué)檢測各組家兔腎組織的TNF-α、IL-10蛋白的表達(dá)水平。 結(jié)里 1、家兔的一般情況 實(shí)驗(yàn)家兔假手術(shù)組與正常組體溫,呼吸頻率變化不明顯,無統(tǒng)計(jì)學(xué)意義(P0.05)；模型組明顯高于正常組及假手術(shù)組(P0.05),烏司他丁組低于模型組(P0.05)。 2、血液檢測指標(biāo) 血細(xì)胞WBC計(jì)數(shù)結(jié)果：實(shí)驗(yàn)家兔正常組與假手術(shù)組比較,差別無統(tǒng)計(jì)學(xué)意義(P0.05)；模型組明顯高于正常組及假手術(shù)組(P0.05),治療組有下降趨勢,有統(tǒng)計(jì)學(xué)意義(P0.05)。 各組血細(xì)胞計(jì)數(shù)(PLT)結(jié)果,差異無統(tǒng)計(jì)學(xué)意義(P().05)。 血清C-反應(yīng)蛋白(CRP)結(jié)果：實(shí)驗(yàn)家兔假手術(shù)組有升高趨勢,與正常組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)；模型組明顯升高,差別有統(tǒng)計(jì)學(xué)意義(P0.05)；治療組與模型組比較明顯下降,差別有統(tǒng)計(jì)學(xué)意義(P0.05)。 家兔血清TNF-α, IL-10含量結(jié)果：與正常組比較,假手術(shù)組家兔血清TNF-α IL-10變化無統(tǒng)計(jì)學(xué)意義(P0.05)；與假手術(shù)組比較,模型組明顯升高(P0.05)；模型組與治療組比較,治療組血清TNF-α明顯降低(P0.05),血清IL-10明顯升高(P0.05)。 3、肝,腎,肺HE染色 實(shí)驗(yàn)家兔正常組和假手術(shù)組組織形態(tài)結(jié)構(gòu)正常。模型組,肝細(xì)胞輕微腫脹,胞漿松弛,有散在炎癥細(xì)胞浸潤；腎小球變形,腎小管管腔變大,腎間質(zhì)有炎癥細(xì)胞浸潤；肺泡間隔彌漫性增厚,有炎癥細(xì)胞浸潤。治療組較模型組上述變化有所減輕。 4. TNF-α,IL-10蛋白免疫組化 家兔正常組、假手術(shù)組腎組織內(nèi)TNF-α,IL-10蛋白主要呈微弱表達(dá)；TNF-α蛋白在模型組表達(dá)最強(qiáng)；IL-10蛋白在治療組表達(dá)最強(qiáng)。 結(jié)論 1、應(yīng)用內(nèi)毒素(LPS)注入輸尿管,并結(jié)扎該側(cè)輸尿管36小時后基本能成功復(fù)制尿源性膿毒癥模型。 2、烏司他丁能有效防治尿源性膿毒癥,減輕組織器官的炎癥反應(yīng),其機(jī)理可能是通過下調(diào)TNF-α,上調(diào)IL-10。
[Abstract]:Objective to establish a rabbit model of ureteral sepsis by injecting lipopolysaccharide (LPS) into the ureter and ligating the ureter, and to investigate the efficacy of ulinastatin in the treatment of sepsis. To provide theoretical and practical basis for the treatment of urinal sepsis with ulinastatin. Methods 1. The animal model of urinary tract obstruction and urinary tract infection with sepsis was established by ligating the left ureter and injecting lipopolysaccharide into the left ureter. 2Twenty four healthy male rabbits were randomly divided into four groups. There were 6 rats in each group. Normal group (Xormal group), sham-operated group (Sham group), model group (model group) and treatment group (UTI group) .3The temperature, respiratory rate, peripheral blood cell count and serum C-reactive protein were monitored after 36 hours of modeling. Serum TNF- 偽 IL-10 was determined by Elisa, liver, kidney and lung tissues were stained with HE, morphological and structural changes of each tissue were observed under optical microscope, and the expression of TNF- 偽 IL-10 protein in renal tissue of rabbits in each group was detected by immunohistochemistry. The general situation of rabbits the temperature and respiratory frequency of the sham operation group and the normal group were not significantly changed (P0.05). Model group was significantly higher than normal group and sham-operation group (P0.05), ulinastatin group was lower than model group (P0.05). Model group was significantly higher than normal group and sham-operation group (P0.05), treatment group had a downward trend, there was statistical significance (P0.05). There was no significant difference in blood cell count (PLT) in each group (P (.05). Serum C-reactive protein (CRP) results: experimental rabbits sham-operation group had an increasing trend, compared with the normal group, the difference was statistically significant (P0.05); the model group significantly increased, the difference was statistically significant (P0.05); treatment group and model group compared with the model group significantly decreased, The difference was statistically significant (P0.05). Results of serum TNF- 偽, IL-10 levels in rabbits: compared with the normal group, the changes of serum TNF- 偽 IL-10 in the sham operation group had no statistical significance (P0.05); compared with the sham operation group, the serum TNF- 偽 IL-10 level in the model group was significantly higher (P0.05); the model group and the treatment group were significantly higher than those in the treatment group. In treatment group, serum TNF- 偽 was significantly decreased (P0.05), serum IL-10 was significantly increased (P0.05). 3. Liver, kidney, lung HE staining of normal rabbits and sham-operation group were normal. In the model group, the hepatocytes were slightly swollen, cytoplasm relaxed, inflammatory cell infiltration scattered; glomerular deformation, tubule lumen becoming larger, interstitial inflammatory cell infiltration; alveolar septum diffuse thickening, inflammatory cell infiltration. Compared with the model group, the above changes in the treatment group were alleviated. 4. 4. The expression of TNF- 偽 and IL-10 protein in renal tissue of sham-operated group was mainly weak. The expression of TNF- 偽 protein in the model group was the strongest, and the expression of IL-10 protein was the strongest in the treatment group. Conclusion 1. Ulinastatin can effectively prevent and treat ureteral sepsis 36 hours after lipopolysaccharide (LPS) injection and ligation of ureter. The mechanism of reducing inflammation in tissues and organs may be through down-regulating TNF- 偽 and upregulating IL-10.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R459.7;R-332

【引證文獻(xiàn)】

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