本文選題：胰島素樣生長因子-1 + 誘導多潛能干細胞��； 參考：《華中科技大學》2012年碩士論文

【摘要】：目的：研究胰島素樣生長因子-1（IGF-1）在iPS細胞重編程過程中的作用。 方法：1.利用含IGF-1的成纖維細胞培養(yǎng)基培養(yǎng)小鼠成纖維細胞3天,此為實驗組。 2.利用不含IGF-1成纖維細胞培養(yǎng)基在相同條件下培養(yǎng)小鼠成纖維細胞3天，此為對照組。 3.分別對兩組成纖維細胞用含Oct4，Sox2，Klf4和c-Myc因子的四種逆 轉錄病毒顆粒進行感染，對形成的iPS細胞進行鑒定。 結果：對照組感染病毒后第21天出現(xiàn)類似胚胎干(embryonic stem；ES)細胞形態(tài)的克隆，共計4個克隆出現(xiàn)，誘導效率約為0.01%。IGF-1處理后的實驗組感染病毒后第18天出現(xiàn)類似ES細胞形態(tài)的克隆，共計15個克隆出現(xiàn)，誘導效率約為0.05%。實驗組的AP染色及SSEA-1、Oct4染色鑒定均為陽性，且誘導效率較對照組提高近5倍。 結論：IGF-1在iPS細胞重編程中發(fā)揮著重要作用，其可以提高四因子體系的誘導效率。 目的：研究胰島素樣生長因子-1(IGF-1)在降低iPS細胞致癌性方面的作用。 方法：1.利用含IGF-1的成纖維細胞培養(yǎng)基培養(yǎng)成纖維細胞3天，此為實驗組。 2.在相同條件下，用不含IGF-1的成纖維細胞培養(yǎng)基培養(yǎng)小鼠成纖維細胞 3天，此為對照組。 3.分別對兩組成纖維細胞用含Oct4，Sox2和Klf4因子的三種逆轉錄病毒顆粒進行感染，對形成的iPS細胞進行鑒定。 結果：對照組經病毒感染后未出現(xiàn)具有ES細胞形態(tài)的克隆。IGF-1處理后的實驗組感染病毒后第20天出現(xiàn)類似ES細胞形態(tài)的克隆，共計3個克隆出現(xiàn)，，誘導效率約為0.01%。實驗組的iPS細胞AP染色及SSEA-1、Oct4染色鑒定均為陽性。 結論：IGF-1在降低iPS細胞的致癌性方面起著重要作用，且聯(lián)合三因子誘導iPS細胞，重編程效率沒有降低。
[Abstract]:Aim: to study the role of insulin-like growth factor-1 (IGF-1) in the reprogramming of iPS cells. Method 1: 1. Mouse fibroblasts were cultured in IGF-1 medium for 3 days. Mouse fibroblasts were cultured in IGF-1 free fibroblast medium under the same conditions for 3 days. The two fibroblasts were infected with four reverse transcriptional virus particles containing Oct4N Sox2Klf4 and c-Myc factor, and the resulting iPS cells were identified. Results: the morphological clones of embryonic stem es cells appeared in the control group on the 21st day after infection. A total of 4 clones appeared. The induction efficiency was about 0.01. IGF-1 treated experimental group showed similar es cell morphology clone on the 18th day after infection. A total of 15 clones appeared and the induction efficiency was about 0.05%. AP staining and SSEA-1 Oct4 staining were positive in the experimental group, and the induction efficiency was 5 times higher than that in the control group. ConclusionTwo one IGF-1 plays an important role in the reprogramming of iPS cells, and it can improve the induction efficiency of the four-factor system. Aim: to study the effect of insulin-like growth factor-1 (IGF-1) on the carcinogenicity of iPS cells. Method 1: 1. The fibroblasts were cultured in IGF-1 medium for 3 days. Under the same conditions, mouse fibroblasts were cultured on fibroblast medium without IGF-1 for 3 days, which was the control group. The two fibroblasts were infected with three kinds of retrovirus particles containing Oct4G Sox2 and Klf4, and the resulting iPS cells were identified. Results: there were no clones with es cell morphology in the control group. The experimental group infected with IGF-1 had similar es cell morphology on the 20th day after infection. A total of 3 clones appeared, and the induction efficiency was about 0.01. AP staining and SSEA-1 Oct 4 staining of iPS cells were positive in the experimental group. Conclusion the weight IGF-1 plays an important role in decreasing the carcinogenicity of iPS cells, and the reprogramming efficiency of iPS cells induced by the combination of three factors is not decreased.
【學位授予單位】：華中科技大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R329.2

【參考文獻】

相關期刊論文 前1條

1 周琪;曾凡一;;通過四倍體補償實驗證明iPS細胞具有發(fā)育全能性[J];中國基礎科學;2010年03期

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