本文選題：銀屑病 + 退銀湯��； 參考：《中國(guó)醫(yī)院藥學(xué)雜志》2017年01期

【摘要】：目的:觀察退銀湯對(duì)咪喹莫特誘導(dǎo)的銀屑病樣小鼠模型中皮損組織和血清中性粒細(xì)胞蛋白酶(NE)、trappin-2、胎盤(pán)型鈣黏蛋白(P-cad)等蛋白表達(dá)水平的干預(yù)作用,探討退銀湯治療銀屑病的作用機(jī)制。方法:使用5%咪喹莫特乳膏外涂小鼠背部脫毛區(qū),制備銀屑病樣小鼠模型;72只小鼠隨機(jī)分為正常對(duì)照組,模型對(duì)照組,退銀湯低、中、高劑量組,消銀顆粒組,每組12只,按照既定實(shí)驗(yàn)藥量給各用藥組灌胃給藥,qd,連續(xù)14 d,正常對(duì)照組和模型對(duì)照組給予等容量的生理鹽水;采用雙抗體酶聯(lián)免疫吸附法檢測(cè)模型小鼠皮損組織及血清中NE、trappin-2、P-cad的含量。結(jié)果:模型組小鼠皮損組織及血清中NE、trappin-2、P-cad的含量均明顯高于正常對(duì)照組(P0.01);退銀湯各劑量組能明顯降低模型小鼠NE、trappin-2、P-cad的表達(dá)水平,與模型對(duì)照組比較均有顯著性差異(P0.05,P0.01);退銀湯中、大劑量組降低更為明顯,與小劑量組比較均有顯著性差異(P0.05,P0.01),并呈現(xiàn)良好的量效依賴關(guān)系;退銀湯大劑量組皮損組織中的上述蛋白水平已接近正常對(duì)照組(P0.05)。結(jié)論:退銀湯能顯著降低咪喹莫特誘導(dǎo)的銀屑病樣小鼠模型NE、trappin-2、P-cad等蛋白的表達(dá)水平,通過(guò)降低上述蛋白的表達(dá)水平來(lái)發(fā)揮調(diào)節(jié)免疫應(yīng)答功能,可能是退銀湯治療銀屑病的作用機(jī)理之一。
[Abstract]:Objective: to observe the effect of Tuiyin decoction (TYD) on the expression level of neutrophil proteinase (neutropenase), placental cadherin (Cam) and other proteins in psoriatic mice model induced by Imiquimote, and to observe the effect of TYT on the expression of protein in skin lesions and serum neutrophil protease. To explore the mechanism of Tuiyin decoction in the treatment of psoriasis. Methods: Seventy-two mice with psoriasis were randomly divided into normal control group, model control group, low, medium, high dose group and Xiaoyin granule group with 12 rats in each group. The normal saline was given to the normal control group and the model control group for 14 days, and the content of netrappin-2 P-cad in the skin lesions and serum of the model mice was detected by double antibody enzyme-linked immunosorbent assay (Elisa). Results: the content of netrappin-2P-cad in skin lesion and serum in model group was significantly higher than that in normal control group (P 0.01), and the expression level of NEtrappin-2 P-cad in model mice was significantly decreased in each dose group, which was significantly different from that in model control group (P 0.05P0.01), and in Tuiyin decoction (TYD), the expression of NEtrappin-2P-cad in model mice was significantly lower than that in model control group. The decrease was more obvious in the large dose group than in the low dose group, there was significant difference between the two groups, and there was a good dose-effect relationship between the two groups, and the protein level in the skin lesions of the large dose group was close to that of the normal control group (P 0.05). Conclusion: TYT can significantly reduce the expression level of netrappin-2pcad and other proteins in psoriatic mice induced by Imiquimod, and regulate the immune response by decreasing the expression level of the above proteins. It may be one of the mechanisms of TYT in treating psoriasis.
【作者單位】： 南通市皮膚病性病防治所;
【基金】：南通市指令性社會(huì)發(fā)展科技計(jì)劃項(xiàng)目(編號(hào):HS2011031)
【分類號(hào)】：R275.9;R-332
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本文編號(hào)：2022394