本文選題：茜草炭 + 化瘀止血��； 參考：《中草藥》2016年08期

【摘要】：目的觀察茜草炭不同提取物對急性血瘀模型大鼠的影響,篩選茜草炭化瘀止血作用最佳的提取方式。方法連續(xù)給藥8 d,于第7天給藥后,除對照組大鼠外,其余各組大鼠均sc鹽酸腎上腺素加冰浴復制急性血瘀模型,以云南白藥為陽性藥,觀察茜草炭醇提物、醇提加水提物、醇提后水提物、水提物對急性血瘀模型大鼠血液流變學、體外血栓形成、血小板系統(tǒng)及纖溶系統(tǒng)的影響。結(jié)果與對照組比較,模型組大鼠各切變率下的全血黏度升高(P0.01),體外血栓濕、干質(zhì)量增加(P0.05),凝血時間延長(P0.05、0.01),血漿纖維蛋白原(FIB)的量與ADP誘導的血小板聚集率升高(P0.01),纖溶酶原激活物抑制物(PAI-1)和血栓素B2(TXB2)的量增加,6-酮-前列腺素1α(6-keto-PGF1α)的量下降(P0.05、0.01)。與模型組比較,茜草炭醇提物與醇提加水提物顯著改善模型大鼠全血黏度、減輕血栓濕質(zhì)量與干質(zhì)量、縮短凝血時間、升高PAI-1與6-keto-PGF1α的量、降低TXB2的量(P0.05、0.01),兩者均能升高模型大鼠血小板聚集率,醇提物顯著降低纖溶酶原激活物(t-PA)的量(P0.05),但對FIB量影響不大;而醇提加水提物顯著升高t-PA的量(P0.01),其中醇提加水提物對全血黏度、血栓濕質(zhì)量與干質(zhì)量影響優(yōu)于醇提物,而醇提物對凝血時間的作用效果強于醇提加水提物;醇提后水提物與水提物對急性血瘀模型大鼠的影響弱于醇提物與醇提加水提提取物,兩者均具有改善模型大鼠全血黏度、減輕血栓干質(zhì)量、縮短凝血酶時間(TT)與凝血酶原時間(PT)、升高PAI-1與TXB2的量、降低t-PA量的趨勢,顯著縮短活化部分凝血活酶時間(APTT)、升高FIB與6-keto-PGF1α的量、減輕血栓濕質(zhì)量(P0.05、0.01)的作用。結(jié)論茜草炭醇提加水提物化瘀止血作用強于茜草炭其他提取物,即醇提加水提法為茜草炭化瘀止血作用的最佳提取方式。
[Abstract]:Objective to observe the effect of different extracts of alizarin charcoal on acute blood stasis model rats and to select the best extraction method for removing blood stasis and hemostatic effect of alizarin charcoal. Methods after 8 days of continuous administration, the rats in all groups except the control group were treated with SC hydrochloric acid epinephrine and ice bath to make acute blood stasis model. Taking Yunnan Baiyao as the positive drug, the alcohol extract and alcohol extract were observed. Effects of water extract after alcohol extraction on hemorheology, thrombus formation in vitro, platelet system and fibrinolytic system in rats with acute blood stasis. Results compared with the control group, the whole blood viscosity of rats in the model group was higher than that in the control group (P 0.01), and the thrombus was wet in vitro. The amount of plasma fibrinogen (FIBs) and platelet aggregation induced by ADP were increased (P 0.01), plasminogen activator inhibitor (PAI-1) and thromboxane B 2 (TXB 2) were increased by increasing the amount of 6-keto-prostaglandin 1 偽 6-kePGF1 偽 (6-kePGF1 偽). Compared with the model group, the alcohol extract of alizarin and ethanol extract significantly improved the whole blood viscosity, reduced the wet and dry weight of thrombus, shortened the coagulation time, and increased the contents of PAI-1 and 6-keto-PGF1 偽. Both of them could increase the platelet aggregation rate of the model rats, and the alcohol extract significantly decreased the amount of plasminogen activator (P0.05N), but had little effect on the FIB content. The water extract of ethanol extract significantly increased the content of t-PA, and the effect of alcohol extract on whole blood viscosity, wet weight and dry weight of thrombus was better than that of alcohol extract, and the effect of alcohol extract on coagulation time was stronger than that on water extract. The effect of water extract and water extract after alcohol extraction on acute blood stasis model rats was weaker than that on alcohol extract and alcohol extraction plus water extract. Both of them could improve the whole blood viscosity and reduce the dry weight of thrombus in the model rats. To shorten the thrombin time (TTT) and prothrombin time (PTT), increase the amount of PAI-1 and TXB2, decrease the amount of t-PA, shorten the activated partial thromboplastin time (APTTT), increase the amount of FIB and 6-keto-PGF1 偽, and reduce the effect of thrombus wet weight (P0.050.01). Conclusion the effect of alcohol extraction and water extract on removing blood stasis and hemostatic effect is stronger than that of other extracts of alizarin charcoal, that is, alcohol extraction and water extraction is the best extraction method for removing stasis and hemostasis of alizarin charcoal.
【作者單位】： 南京中醫(yī)藥大學江蘇省中藥資源產(chǎn)業(yè)化過程協(xié)同創(chuàng)新中心/中藥資源產(chǎn)業(yè)化與方劑創(chuàng)新藥物國家地方聯(lián)合工程研究中心;
【基金】：國家自然科學基金資助項目(81473348) 江蘇高校優(yōu)勢學科建設(shè)工程資助項目(ysxk-2014) 江蘇高校品牌專業(yè)建設(shè)工程資助項目(PPZY2015A070)
【分類號】：R285.5;R-332

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