發(fā)布時(shí)間：2018-06-10 11:15

  本文選題：秀麗線蟲(chóng) + ROS　； 參考：《西北農(nóng)林科技大學(xué)》2012年碩士論文

【摘要】：氧化應(yīng)激是指機(jī)體在遭受各種有害刺激時(shí),體內(nèi)高活性分子如活性氧簇(reactive oxygen species, ROS)產(chǎn)生過(guò)多,氧化程度超出氧化物的清除,氧化系統(tǒng)和抗氧化系統(tǒng)失衡,從而導(dǎo)致組織損傷。氧化應(yīng)激是由ROS在體內(nèi)產(chǎn)生的一種負(fù)面作用,并被認(rèn)為是導(dǎo)致衰老和疾病的一個(gè)重要因素,研究報(bào)道ROS在癲癇、高血壓、動(dòng)脈粥樣硬化、帕金森病、猝死中均扮演重要角色。因此闡明氧化應(yīng)激機(jī)制及相關(guān)基因功能將對(duì)疾病治療及抗氧化藥物的篩選有重要的意義。 本研究利用模式生物秀麗線蟲(chóng),進(jìn)行了如下相關(guān)工作： 首先,通過(guò)次氯酸處理?xiàng)l件的摸索建立次氯酸氧化應(yīng)激模型,熒光探針檢測(cè)次氯酸處理后ROS水平,次氯酸處理后抗氧化劑NAC對(duì)氧化損傷保護(hù)作用的檢測(cè),同時(shí)還對(duì)不同突變株系的次氯酸敏感性進(jìn)行檢測(cè)。結(jié)果表明,次氯酸可以作為氧化應(yīng)激誘導(dǎo)劑用于新的氧化應(yīng)激模型,daf-2、egl-9、hif-1、vhl-1可能參與次氯酸氧化應(yīng)激信號(hào)通路的應(yīng)答。 其次,構(gòu)建了glb-13Is株系,從蛋白水平.mRNA水平證明PQ處理后線蟲(chóng)體內(nèi)glb-13表達(dá)水平升高,通過(guò)轉(zhuǎn)基因株系Is4、雜交株系Is4；tm等證明hNgb對(duì)線蟲(chóng)Glb-13有補(bǔ)償作用。結(jié)果表明,秀麗線蟲(chóng)glb-13基因具有與hNgb相同的抗百草枯誘導(dǎo)的氧化損傷功能。 總上所述,本研究建立了一個(gè)新的氧化應(yīng)激模型,豐富了秀麗線蟲(chóng)氧化應(yīng)激機(jī)制研究,為抗氧化藥物篩選及疾病治療提供了理論基礎(chǔ)。
[Abstract]:Oxidative stress refers to the excessive production of highly active molecules such as reactive oxygen specieses (ROSs) when the body is exposed to various harmful stimuli. The degree of oxidation exceeds the scavenging of oxides and the imbalance between the oxidation system and the antioxidant system leads to tissue damage. Oxidative stress is a negative effect of Ros in vivo and is considered to be an important factor leading to aging and disease. It has been reported that Ros plays an important role in epilepsy, hypertension, atherosclerosis, Parkinson's disease and sudden death. Therefore, the elucidation of oxidative stress mechanism and related gene functions will play an important role in the treatment of diseases and the screening of antioxidant drugs. The oxidative stress model of hypochloric acid was established by exploring the conditions of hypochloric acid treatment. The Ros level after hypochloric acid treatment and the protective effect of antioxidant NAC on oxidative damage were detected by fluorescence probe. At the same time, the sensitivity of different mutant lines to hypochloric acid was tested. The results showed that hypochloric acid could be used as an oxidative stress inducer for a new oxidative stress model: daf-2egl-9hif-1hl-1 might be involved in the response of hypochloric acid oxidative stress signaling pathway. Secondly, glb-13Is strain was constructed. The protein level and mRNA level of nematodes increased after PQ treatment. The results showed that hNgb could compensate the nematode Glb-13 by transgenic line Is4 and hybrid line Is4tm. The results showed that the glb-13 gene had the same function as hNgb in resisting oxidative damage induced by paraquat. A new oxidative stress model was established in this study, which enriched the study of oxidative stress mechanism of nematode. It provides a theoretical basis for the screening of antioxidation drugs and the treatment of diseases.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李婷婷;彭軍;;次氯酸介導(dǎo)的氧化應(yīng)激及其病理生理學(xué)意義[J];中國(guó)藥理學(xué)與毒理學(xué)雜志;2010年04期

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本文編號(hào)：2003019