本文選題：大鼠 + 丘腦背外側(cè)核��； 參考：《大連醫(yī)科大學(xué)》2011年碩士論文

【摘要】：大鼠丘腦背外側(cè)核(LD)是丘腦外側(cè)核群的組成部分,與空間學(xué)習(xí)和記憶有關(guān)。其又分為背內(nèi)側(cè)部(LDDM)和腹外側(cè)部(LDVL)。5-羥色胺(5-HT)作為重要的神經(jīng)遞質(zhì)與其2A型受體(5-HTR_(2A))也被認為在學(xué)習(xí)和記憶過程中發(fā)揮著非常重要的作用。 目的:本實驗應(yīng)用免疫組化技術(shù),通過觀察5-羥色胺及其2A受體在大鼠丘腦背外側(cè)核不同部位的表達情況,為進一步研究丘腦背外側(cè)核、5-羥色胺及其2A受體與學(xué)習(xí)記憶的關(guān)系提供形態(tài)學(xué)資料。 方法:實驗動物采用SD成年大鼠8只,雌雄各半,體重200～250g。由大連醫(yī)科大學(xué)實驗動物中心提供。4%水合氯醛腹腔麻醉(1ml/100g體重),仰臥固定,用剪刀剪開胸壁暴露心臟,將灌注針經(jīng)左心室插至升主動脈。剪開右心耳,快速灌注生理鹽水200ml直至右心耳流出液變得無色透明,再灌注4%多聚甲醛固定液(pH7.4)300ml,前100ml快速灌注,后200ml緩慢灌注,持續(xù)灌注約2h。灌畢立即取腦,剔凈腦表面的腦膜,置于4%多聚甲醛固定液中4℃過夜,然后用振動切片機連續(xù)冠狀切片,片厚30μm。切取3套,一套用于檢測5-HT,一套用于檢測5-HTR_(2A),一套做對照實驗。采用免疫組織化學(xué)染色方法,光鏡下觀察5-HT及5-HTR_(2A)在丘腦背外側(cè)核不同部位的表達情況,所有標(biāo)本均使用同一套圖像分析系統(tǒng)進行拍照并應(yīng)用圖像分析系統(tǒng)進行分析,所得數(shù)據(jù)應(yīng)用SPSS11.5軟件進行配對資料t檢驗。 結(jié)果:1. 5-HT免疫組化結(jié)果:光鏡下,大鼠丘腦背外側(cè)核LDDM和LDVL兩部位均可見棕黃色免疫反應(yīng)陽性的5-HT能神經(jīng)纖維終末,高倍鏡下可見纖維終末走行距離較長,呈串珠狀。LDDM和LDVL兩部位平均光密度值比較,數(shù)值無統(tǒng)計學(xué)差異(P0.05)。陰性對照未見陽性表達。2. 5-HTR_(2A)免疫組化結(jié)果:在丘腦背外側(cè)核LDDM和LDVL兩部位均可見棕黃色的免疫反應(yīng)陽性神經(jīng)元,分布均勻,其胞體呈中等大小,免疫反應(yīng)陽性產(chǎn)物主要定位于神經(jīng)元胞膜,胞質(zhì)和胞核不著色。LDDM和LDVL兩部位平均灰度值比較,數(shù)值無統(tǒng)計學(xué)差異(P0.05)。陰性對照未見陽性表達。 結(jié)論:在大鼠丘腦背外側(cè)核內(nèi)存在5-HT能神經(jīng)纖維終末。5-HTR_(2A)在大鼠丘腦背外側(cè)核內(nèi)有表達。
[Abstract]:LDD is an integral part of the lateral thalamic nucleus group, which is related to spatial learning and memory. As an important neurotransmitter and its type 2A receptor 5-HTR, LDDM (LDDM) and LDVLA (5-HT) are also considered to play an important role in the process of learning and memory. Objective: to observe the expression of 5-hydroxytryptamine and its 2A receptor in different parts of the dorsal lateral nucleus of the thalamus by immunohistochemical technique. To provide morphological data for further study of the relationship between 5-hydroxytryptamine and its 2A receptor and learning and memory in dorsal lateral thalamic nucleus. Methods: eight adult SD rats, half male and female, were used. An intraperitoneal anaesthesia of 1 ml / 100 g of chloral hydrate was provided by the Experimental Animal Center of Dalian Medical University. After supine fixation, the chest wall was cut open with scissors to expose the heart, and the perfusion needle was inserted into the ascending aorta through the left ventricle. The right atrial appendage was cut open, the normal saline 200ml was infused quickly until the effluent of the right atrial appendage became colorless and transparent, and the pH value of 4% paraformaldehyde fixed solution was 7.4 ~ 300ml after reperfusion. The 100ml was infused rapidly before and after 200ml was infused slowly for about 2 hours. The brain was removed immediately after perfusion, the meninges on the surface of the brain were removed and placed at 4 鈩，

本文編號：1954335