本文選題：仙茅苷 + 血管性癡呆��； 參考：《神經(jīng)解剖學(xué)雜志》2017年04期

【摘要】：目的:研究仙茅苷對血管性癡呆(VD)模型大鼠海馬區(qū)Caspase-3、PARP-1和雌激素受體(ER)表達(dá)的作用。方法:確立SD大鼠對照組后,用構(gòu)建的血管性癡呆模型大鼠隨機(jī)分成模型組、藥物仙茅苷低(24 mg/kg)和高劑量(72 mg/kg)組(予以仙茅苷灌胃4周),每組8只。用藥結(jié)束后,按設(shè)計用Morris水迷宮測試大鼠空間學(xué)習(xí)記憶功能;流式細(xì)胞術(shù)分析海馬區(qū)神經(jīng)細(xì)胞元凋亡;Caspase-3、PARP-1和ER蛋白水平用Western Blot測定;Caspase-3、PARP-1和ER mRNA表達(dá)用Realtime PCR檢測。結(jié)果:Morris水迷宮試驗結(jié)果顯示,藥物組和模型組大鼠逃避潛伏期均明顯延長(P0.05~0.01),模型組大鼠更明顯(P0.01);藥物組和模型組大鼠空間探索距離百分比均降低(P0.05~0.01),模型組大鼠降更明顯(P0.01)。與對照組比較,藥物組和模型組大鼠海馬神經(jīng)細(xì)胞凋亡率顯著提高(P0.01),模型組大鼠更明顯(P0.01)。與對照組比較,模型組的Caspase-3、PARP-1蛋白和mRNA表達(dá)均增加(P0.05);與模型組相比,仙茅苷各劑量組可見ER表達(dá)增加(P0.05),Caspase-3和PARP-1表達(dá)降低(P0.05)。與對照組比較,模型組的Caspase-3和PARP-1表達(dá)增加(P0.01),而ER未見明顯變化(P0.05);與模型組相比,仙茅苷各劑量組ER表達(dá)均增加(P0.01),Caspase-3和PARP-1表達(dá)不明顯(P0.05),但不同劑量仙茅苷組間未見明顯差異(P0.05)。結(jié)論:結(jié)果表明仙茅苷具有改善血管性模型大鼠空認(rèn)知功能的作用是通過抑制海馬區(qū)神經(jīng)細(xì)胞凋亡,下調(diào)Caspase-3和PARP-1表達(dá),上調(diào)海馬ER表達(dá)實現(xiàn)的。
[Abstract]:Aim: to study the effect of citronelin on the expression of Caspase-3, PARP-1 and estrogen receptor (ERR) in hippocampus of vascular dementia rats. Methods: Sprague-Dawley rats (SD rats) were randomly divided into two groups: the model group (24 mg / kg) and the high dose group (72 mg / kg). After treatment, the spatial learning and memory function of rats was measured by Morris water maze, and the expression of Caspase-3, PARP-1 and ER mRNA in hippocampal neurons were analyzed by flow cytometry. The expression of Caspase-3, PARP-1 and ER mRNA was detected by Realtime PCR. Results the results of the water maze test showed that the escape latency of both the drug group and the model group was significantly longer than that of the model group, and that of the model group was more obvious than that of the model group, and that of the drug group and the model group was significantly lower than that of the drug group and the model group, and the percentage of the space exploration distance of the drug group and the model group was lower than that of the model group, and that of the model group was more obvious than that of the model group. Compared with the control group, the apoptotic rate of hippocampal neurons in the drug group and the model group was significantly increased, and that in the model group was more obvious than that in the model group. Compared with the control group, the expression of Caspase-3, PARP-1 and mRNA increased in the model group, while the expression of ER increased and the expression of Caspase-3 and PARP-1 decreased in the model group compared with the model group. Compared with the control group, the expression of Caspase-3 and PARP-1 increased in the model group, but there was no significant change in ER. Compared with the model group, the expression of Caspase-3 and PARP-1 was not significantly increased in each dose group, but there was no significant difference between the different dose groups. Conclusion: the results suggest that citronelin can improve the spatial cognitive function of vascular model rats by inhibiting the apoptosis of hippocampal neurons, down-regulating the expression of Caspase-3 and PARP-1, and up-regulating the expression of ER in hippocampus.
【作者單位】： 慶陽市人民醫(yī)院神經(jīng)內(nèi)科;隴東學(xué)院岐伯醫(yī)學(xué)院;解放軍169醫(yī)院消化血液科;西安醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)研究所;
【基金】：國家自然科學(xué)基金(81471265) 陜西省科技統(tǒng)籌創(chuàng)新工程計劃項目(2012KTCG01-02)
【分類號】：R285.5;R-332

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