發(fā)布時間：2018-05-24 08:57

  本文選題：A族鏈球菌(GAS) + 多價疫苗��； 參考：《中國免疫學(xué)雜志》2017年03期

【摘要】：目的:完善A族鏈球菌(GAS)多價疫苗并探究其免疫保護(hù)效果。方法:構(gòu)建GAS的多肽疫苗F7M6,將其中的M6多肽克隆作為對照,表達(dá)純化后,將兩種蛋白連同本室保存的GAS M1蛋白分別免疫小鼠,末次免疫后10 d,半數(shù)小鼠行ELISPOT檢測IL-4和IFN-γ,ELISA檢測各組的Ig G、Ig G1、Ig G2a;同時,以F7M6蛋白為診斷抗原檢測其與抗鏈"O"陽性人血清的特異性結(jié)合;致死量的M1GAS攻毒并計算存活率。結(jié)果:無論是誘導(dǎo)細(xì)胞免疫,還是誘導(dǎo)抗體產(chǎn)生,F7M6組的水平均為最高;攻毒后F7M6蛋白的生存率為66.7%。F7M6蛋白檢測抗鏈"O"陽性血清的陽性檢出率為95%。結(jié)論:GAS的多肽疫苗F7M6能誘導(dǎo)特異性細(xì)胞和體液免疫應(yīng)答,對GAS感染具有良好的保護(hù)效果,且F7M6的多價表位涵蓋于時下流行的大部分GAS的血清型中。
[Abstract]:Objective: to improve the multivalent vaccine of group A streptococcus sp. Methods: the peptide vaccine F7M6 of GAS was constructed, and the M6 peptide was cloned as control. After expression and purification, mice were immunized with the two proteins together with the GAS M1 protein stored in our laboratory. Ten days after the last immunization, half of the mice were tested for IL-4 and IFN- 緯 -ELISA for IL-4 and IFN- 緯 immunosorbent assay, and F7M6 protein was used as diagnostic antigen to detect its specific binding to anti-chain "O" positive human serum, and the lethal amount of M1GAS was used to attack the virus and calculate the survival rate. Results: the highest level of F7M6 was found in the group of inducing cellular immunity and antibody production, and the survival rate of F7M6 protein after attack was 95%. The positive rate of anti-chain "O" positive serum detected by 66.7%.F7M6 protein was 95%. Conclusion F7M6 can induce specific cellular and humoral immune responses and has a good protective effect against GAS infection. The polyvalent epitopes of F7M6 are contained in the serotypes of most popular GAS.
【作者單位】： 河北醫(yī)科大學(xué)免疫教研室河北省重大疾病的免疫機制及干預(yù)重點實驗室;河北醫(yī)科大學(xué)第四醫(yī)院;
【基金】：國家自然科學(xué)基金(31370914和81172810) 河北省自然科學(xué)基金(H2016206516)資助
【分類號】：R392

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本文編號：1928457