發(fā)布時間：2018-05-03 00:13

  本文選題：惡性瘧原蟲 + 體外培養(yǎng)　； 參考：《大理學(xué)院》2011年碩士論文

【摘要】：目的1.觀察鑒定體外培養(yǎng)瘧原蟲過程中容易污染的細(xì)菌種類、來源及藥物敏感性,初步探尋控制污染的方法。2.探尋紫外線照射和甲醛熏蒸實驗室內(nèi)空氣消毒效果。方法在采用CO2混合氣體法人工培養(yǎng)惡性瘧原蟲過程中,對被污染的培養(yǎng)物進(jìn)行細(xì)菌鑒定；對操作者手指部進(jìn)行表面采樣法培養(yǎng)鑒定。采用每天84消毒液拖地、新潔爾滅擦拭門和操作臺,延長實驗室紫外線輻射和操作前新潔爾滅洗手、操作中物體表面噴灑酒精等措施進(jìn)行消毒控制。對實驗室內(nèi)空氣采用自然沉降法采樣培養(yǎng)鑒定；實驗室內(nèi)甲醛熏蒸前和之后每隔24h進(jìn)行空氣采樣1次,連續(xù)進(jìn)行1周,間隔一定時期后重復(fù)上述采樣過程。結(jié)果培養(yǎng)過程中培養(yǎng)物污染的細(xì)菌為嗜麥芽寡養(yǎng)單胞菌Sernotrphomonas maltophilia、鮑曼氏/醋酸鈣不動桿菌復(fù)合體A. baumannii / calcoaceticus complex和魯氏不動桿菌Acinetobacter lwoffii (acilwo),均為革蘭陰性桿菌(G-b),且屬于多重耐藥菌株；實驗室不同時期空氣中細(xì)菌構(gòu)成不同,初期檢測發(fā)現(xiàn)以桿菌為主,如假單胞菌Pseudomonas、鮑曼氏不動桿菌A. baumannii、陰溝腸桿菌E. cloacae等,后期檢測發(fā)現(xiàn)主要為革蘭陽性球菌(G+c),特別是藤黃微球菌M. luteus和解糖葡萄球菌S. saccharolyticus,均對慶大霉素敏感；手部攜帶菌株為常見的表皮葡萄球菌S. epidermidi和藤黃微球菌M. luteus。甲醛熏蒸和增加紫外線照射時間均能夠減少空氣中細(xì)菌數(shù)量。結(jié)論通過加強(qiáng)消毒措施同時應(yīng)用慶大霉素可以很好地控制體外培養(yǎng)瘧原蟲時出現(xiàn)的細(xì)菌污染。影響紫外線照射效果因素極多,并且影響自然沉降法空氣采樣結(jié)果之因素亦較多,紫外線照射時間與空氣中細(xì)菌數(shù)量之間的曲線不易準(zhǔn)確繪出。甲醛熏蒸能夠很好減少空氣中細(xì)菌數(shù)量,但隨著時間延長效果逐步減弱,且熏蒸后24～48h內(nèi)氣味較重,影響工作人員身體。
[Abstract]:Objective 1. To observe and identify the species, sources and drug sensitivity of bacteria liable to contamination during the culture of Plasmodium in vitro, and to explore the method of pollution control. 2. To explore the effect of ultraviolet irradiation and formaldehyde fumigation in laboratory air disinfection. Methods in the process of artificial culture of Plasmodium falciparum by CO2 mixed gas method, the contaminated cultures were identified, and the surface samples of the operator's fingers were cultured and identified. Every day 84 disinfectant was used to mop the ground, Xinjier wiped the door and the operating table, prolonged the ultraviolet radiation in the laboratory, washed hands before the operation, and sprayed alcohol on the surface of the object in operation to carry on the disinfection control. The air in the laboratory was sampled and identified by natural sedimentation method, and the air was sampled once every 24 hours before and after formaldehyde fumigation in the laboratory, and the sampling process was repeated after a certain interval for one week. Results the bacteria contaminated by culture were Sernotrphomonas maltophilia, Acinetobacter baumannii / calcium Acinetobacter complex A. baumannii / calcoaceticus complex and Acinetobacter rubrum Acinetobacter lwoffii. The composition of bacteria in the air was different in different periods of the laboratory. The main bacteria were Pseudomonas pseudomonas, Acinetobacter baumannii, Enterobacter cloacae E. cloacae, and so on. It was found that Gram-positive cocci, especially Micrococcus luteus and S.saccharolyticus, were sensitive to gentamicin, and the common strains were S. epidermidis epidermidi and M. luteus. Formaldehyde fumigation and increasing UV exposure time can reduce the number of bacteria in the air. Conclusion Gentamicin can effectively control the bacterial contamination of Plasmodium falciparum cultured in vitro by strengthening disinfection measures at the same time. There are many factors that influence the effect of ultraviolet radiation, and there are also many factors that affect the air sampling results by natural sedimentation method. The curve between ultraviolet irradiation time and bacteria quantity in air is not easy to draw accurately. Formaldehyde fumigation could reduce the number of bacteria in the air, but the effect of formaldehyde fumigation decreased gradually with the prolongation of time, and the smell of formaldehyde fumigation was more serious within 24 ~ 48 hours after fumigation, which affected the health of the workers.
【學(xué)位授予單位】：大理學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R378

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