本文選題：結(jié)核分枝桿菌 + 毒素-抗毒素系統(tǒng)　； 參考：《中國(guó)人獸共患病學(xué)報(bào)》2017年02期

【摘要】：目的探討結(jié)核分枝桿菌毒素基因mazF3,6,9及抗毒素基因mazE3,6,9的表達(dá)差異。方法運(yùn)用實(shí)時(shí)定量PCR檢測(cè)結(jié)核分枝桿菌單耐藥株20株,耐多藥株20株和標(biāo)準(zhǔn)株H37Rv毒素基因mazF3,6,9及抗毒素基因mazE3,6,9的表達(dá)水平;組間基因表達(dá)水平的差異用one-way ANOVA進(jìn)行統(tǒng)計(jì)分析。結(jié)果與對(duì)照株相比較,毒素基因mazF6,9在單耐藥組(11.151 9±22.317 21;8.430 6±17.978 97)及耐多藥組(4.601 6±1.290 18;6.962 7±6.929 48)表達(dá)均上調(diào)且差異有統(tǒng)計(jì)學(xué)意義(P0.01),mazF3基因在單耐藥組及耐多藥組差異無統(tǒng)計(jì)學(xué)意義(P0.05),抗毒素基因mazE3在單耐藥(0.360 6±0.125 27)及耐多藥組(0.201 6±0.165 42)中表達(dá)均下調(diào),差異有統(tǒng)計(jì)學(xué)意義(P0.01),mazE6均無統(tǒng)計(jì)學(xué)意義,mazE9只有在耐多藥組(0.398 9±0.376 79)中表達(dá)下調(diào),差異有統(tǒng)計(jì)學(xué)意義,(P0.01)。結(jié)論毒素基因mazF6,9抗毒素基因mazE3,9可能參與了結(jié)核分枝桿菌的耐藥形成,具體機(jī)制尚待進(jìn)一步的研究。
[Abstract]:Objective to investigate the difference between the expression of Mycobacterium tuberculosis toxin gene mazF3O6O9 and the anti-toxin gene mazE3O6O9. Methods Real-time quantitative PCR was used to detect the expression levels of the H37Rv toxin gene mazF3O6N9 and the anti-toxin gene mazE3O6N9 in 20 strains of mycobacterium tuberculosis, 20 strains of multidrug resistance and 20 strains of standard strains, and the difference of gene expression between groups was analyzed by one-way ANOVA. Results compared with the control plant, The expression of the toxin gene mazF6n9 was up-regulated in the single drug resistance group (11.1559 鹵22.317 21) and the multidrug resistance group (4.6016 鹵1.290 186.9627 鹵6.929 48), and there was no significant difference in the expression of the toxin gene maz F3 gene in the single drug resistance group and the multidrug resistance group (P 0.05), while the mazE3 of the antitoxin gene was not significantly different in the single drug resistance group and the multidrug resistance group. The expression of YD in 0.360 6 鹵0.125 27) and multidrug resistant group (0.201 6 鹵0.165 42) was down-regulated. There was no significant difference in the expression of maz E9 in multidrug resistant group (P 0.01), and the difference was statistically significant (P 0.01) only in the multidrug resistant group (0.398 9 鹵0.376 79). Conclusion the anti-toxin gene mazE3O9 may be involved in the formation of drug resistance of Mycobacterium tuberculosis, and the specific mechanism remains to be further studied.
【作者單位】： 石河子大學(xué)醫(yī)學(xué)院免疫學(xué)教研室;
【基金】：國(guó)家自然科學(xué)基金(No.81341079,81560264)資助~~
【分類號(hào)】：R378.911

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