本文選題：再生障礙性 + 動(dòng)物模型��； 參考：《南通大學(xué)》2012年碩士論文

【摘要】：目的探討調(diào)節(jié)性T細(xì)胞和Th17細(xì)胞在干擾素-γ(interferon-γ, IFN-γ)聯(lián)合白消安誘導(dǎo)的新型小鼠重型再生障礙性貧血(再障)模型中的變化,從免疫學(xué)水平上論證該模型合理性。 方法應(yīng)用IFN-γ腹腔注射聯(lián)合白消安灌胃誘導(dǎo)建立BALB/c小鼠重型再障模型(聯(lián)合組,n=15),以白消安組(n=15)、IFN-γ組(n=15)和正常組(n=15)為對(duì)照,分別收集各組小鼠的外周血和脾臟中的單個(gè)核細(xì)胞,采用流式細(xì)胞術(shù)檢測(cè)各組小鼠外周血和脾臟中CD4+CD25+Foxp3+調(diào)節(jié)性T細(xì)胞(Treg)和Th17細(xì)胞的比例變化,并逐層分析比較。 結(jié)果聯(lián)合組小鼠外周血和脾臟的調(diào)節(jié)性T細(xì)胞的百分比分別為(13.19±0.76)%和(4.77±1.05)%,較正常組、白消安組、IFN-γ組顯著降低(P值均0.()1)：而Th17細(xì)胞百分比分別為(2.07±0.12)%和(3.18±0.46)%,較其他三組均顯著升高(P值分別0.05和0.01)。 結(jié)論IFN-γ聯(lián)合白消安誘導(dǎo)建立的重型再障小鼠調(diào)節(jié)性T細(xì)胞數(shù)量降低而Th17細(xì)胞升高,提示該小鼠重型再障模型可能更接近并模擬了人類(lèi)免疫介導(dǎo)的骨髓造血功能損傷,可以為今后的實(shí)驗(yàn)提供可能的理論依據(jù)。
[Abstract]:Objective to investigate the changes of regulatory T cells and Th17 cells in a new mouse model of severe aplastic anemia (aplastic anemia) induced by interferon- 緯 (IFN- 緯) and oxacol, and to demonstrate the rationality of the model from the immunological level. Methods the model of severe aplastic anemia in BALB/c mice was established by intraperitoneal injection of IFN- 緯 and oral administration of Paixiaan. Mononuclear cells were collected from peripheral blood and spleen of BALB/c mice. The percentage of CD4 CD25 Foxp3 regulatory T cells and Th17 cells in peripheral blood and spleen of each group were detected by flow cytometry. Results the percentages of regulatory T cells in peripheral blood and spleen of the combined group were 13.19 鹵0.76% and 4.77 鹵1.05%, respectively, which were higher than those in the normal group. The percentage of Th17 cells were 2.07 鹵0.12% and 3.18 鹵0.46%, respectively, which were significantly higher than those of the other three groups. Conclusion the number of regulatory T cells decreased and Th17 cells increased in mice with severe aplastic anemia induced by IFN- 緯 and oxacol, suggesting that the model of severe aplastic anemia in mice may be closer to and mimic the human immune-mediated hematopoietic impairment of bone marrow. It can provide possible theoretical basis for future experiments.
【學(xué)位授予單位】：南通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R556.5;R-332

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本文編號(hào)：1804535