本文選題：多烯磷脂酰膽堿 + 樹突狀細胞��； 參考：《中國現(xiàn)代藥物應(yīng)用》2016年24期

【摘要】：目的研究多烯磷脂酰膽堿(PPC)對樹突狀細胞(DC)表型分化及分泌炎癥因子的影響,探討PPC抗炎作用。方法 8只SPF紋雌性Balb/c小鼠,隨機分為四組,每組2只。運用免疫磁珠分析小鼠脾臟DC,分別加入PPC(PPC組,2只)、脂多糖(LPS,LPS組,2只)、PPC+LPS(PPC+LPS組,2只)以及磷酸鹽緩沖液(PBS,PBS組,2只)進行刺激。24 h后收集細胞及培養(yǎng)上清,流式細胞術(shù)檢測DC表面活化分子表達情況,流式微珠陣列法(CBA)檢測細胞因子分泌情況。結(jié)果 PPC組DC表面CD40、CD80、CD86、主要組織相容性復合體(MHC)-Ⅱ分別為(23.43±2.79)%、(19.41±1.33)%、(44.30±3.16)%、(80.87±1.32)%,低于PBS組的(29.10±8.14)%、(31.81±1.02)%、(47.70±6.32)%、(83.17±2.84)%;且顯著低于LPS組的(42.33±0.58)%、(63.57±2.61)%、(56.07±0.06)%、(86.20±0.95)%(P0.01);PPC+LPS組CD40、CD80、CD86、MHC-Ⅱ分別為(16.73±3.14)%、(16.12±1.92)%、(43.60±0.95)%、(78.83±1.46)%,顯著低于LPS組(P0.01)。PPC組和PPC+LPS組白細胞介素(IL)-6、腫瘤壞死因子(TNF)顯著低于LPS組(P0.01)。PPC+LPS組單核細胞趨化蛋白-1(MCP-1)水平明顯低于LPS組(P0.01)。結(jié)論 PPC可通過抑制DC活化及炎癥因子釋放,發(fā)揮抗炎效果。
[Abstract]:Objective to investigate the effect of polyenylphosphatidylcholine (PPC) on phenotype differentiation and secretion of inflammatory cytokines in dendritic cells (DC). Methods eight SPF striated female Balb/c mice were randomly divided into four groups with 2 mice in each group. The spleen of mice was analyzed by immunomagnetic beads. The cells were collected and cultured in PPC(PPC group (n = 2), lipopolysaccharide (LPS) group (n = 2) and PPC LPS(PPC LPS group (n = 2) and phosphate buffer solution (PBS) group (n = 2). Flow cytometry was used to detect the expression of activated molecules on DC surface and CBA was used to detect cytokine secretion. 緇撴灉 PPC緇凞C琛ㄩ潰CD40,CD80,CD86,涓昏緇勭粐鐩稿鎬у鍚堜綋(MHC)-鈪″垎鍒負(23.43鹵2.79)%,(19.41鹵1.33)%,(44.30鹵3.16)%,(80.87鹵1.32)%,浣庝簬PBS緇勭殑(29.10鹵8.14)%,(31.81鹵1.02)%,(47.70鹵6.32)%,(83.17鹵2.84)%;涓旀樉钁椾綆浜嶭PS緇勭殑(42.33鹵0.58)%,(63.57鹵2.61)%,(56.07鹵0.06)%,(86.20鹵0.95)%(P0.01);PPC LPS緇凜D40,CD80,CD86,MHC-鈪″垎鍒負(16.73鹵3.14)%,(16.12鹵1.92)%,(43.60鹵0.95)%,(78.83鹵1.46)%,鏄捐憲浣庝簬LPS緇，

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