本文選題：CDH + cisplatin��； 參考：《廣東醫(yī)學(xué)》2017年15期

【摘要】：目的研究下調(diào)CDH17基因?qū)isplatin耐藥的人胃癌BGC823細(xì)胞裸鼠移植瘤模型的影響,從體內(nèi)實(shí)驗(yàn)研究治療胃癌新的治療手段。方法以人胃癌BGC823細(xì)胞為研究材料,建立人胃癌BGC823細(xì)胞裸鼠移植瘤模型。同時(shí),構(gòu)建干擾質(zhì)粒從基因和蛋白水平對(duì)CDH17進(jìn)行干擾,成功構(gòu)建慢病毒載體。將細(xì)胞分為三組,分別是WT對(duì)照組,si NC陰性對(duì)照組,si CDH17干擾組。通過(guò)TUNEL方法和Annexin V/PI雙染色法檢測(cè)各組細(xì)胞的凋亡水平。結(jié)果 Real-time PCR和蛋白免疫印跡實(shí)驗(yàn)證實(shí)成功構(gòu)建了CDH17的干擾表達(dá)載體。TUNEL檢測(cè)實(shí)驗(yàn)表明下調(diào)CDH17基因可以促進(jìn)對(duì)cisplatin耐藥的人胃癌BGC823細(xì)胞裸鼠移植瘤模型細(xì)胞凋亡。Annexin V/PI雙染色檢測(cè)si CDH17干擾組細(xì)胞早期凋亡率遠(yuǎn)高于WT對(duì)照組和si NC陰性對(duì)照組的細(xì)胞。結(jié)論下調(diào)CDH17基因可以促進(jìn)cisplatin耐藥性人胃癌BGC823細(xì)胞凋亡,抑制腫瘤細(xì)胞增殖而達(dá)到治療胃癌的目的。
[Abstract]:Objective to study the effect of down-regulation of CDH17 gene on the transplanted tumor model of human gastric cancer BGC823 cells resistant to cisplatin in nude mice, and to study a new therapeutic method for gastric cancer in vivo. Methods Human gastric cancer BGC823 cells were used as materials to establish nude mice model of transplanted tumor of human gastric cancer BGC823 cells. At the same time, the interference plasmid was constructed to interfere with CDH17 from gene and protein levels, and the lentivirus vector was successfully constructed. The cells were divided into three groups, namely WT control group and control group. The apoptosis level was detected by TUNEL and Annexin V/PI double staining. Results Real-time PCR and Western blot confirmed that the interference expression vector of CDH17 was successfully constructed. Tunel assay showed that down-regulation of CDH17 gene could promote apoptosis. Annexin V/PI of cisplatin resistant human gastric cancer BGC823 cells in nude mice. The early apoptosis rate of si CDH17 interference group was much higher than that of WT control group and si NC negative control group. Conclusion down-regulation of CDH17 gene can promote the apoptosis of BGC823 cells and inhibit the proliferation of gastric cancer cells in cisplatin resistant human gastric cancer.
【作者單位】： 湖北省武漢市第三醫(yī)院;
【基金】：湖北省自然科學(xué)基金資助項(xiàng)目(編號(hào):2013CFB358) 武漢市衛(wèi)生計(jì)劃生育委員會(huì)科研項(xiàng)目(編號(hào):WX14C18)
【分類(lèi)號(hào)】：R-332;R735.2

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