本文選題：電場 + CXCR4��； 參考：《重慶醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的探討生理強(qiáng)度的電場(250mv/mm)刺激對大鼠胎鼠神經(jīng)干細(xì)胞受體CXCR4和NMDAR1的表達(dá)及相互作用的影響,并研究其可能的作用機(jī)制。 方法體外分離培養(yǎng)大鼠胎鼠的神經(jīng)干細(xì)胞并施加電場,免疫熒光化學(xué)方法鑒定神經(jīng)干細(xì)胞；western印跡和細(xì)胞免疫熒光化學(xué)方法檢測電場刺激前后神經(jīng)干細(xì)胞表達(dá)CXCR4和NMDAR1的情況；免疫共沉淀和細(xì)胞免疫熒光化學(xué)方法檢測電場刺激前后神經(jīng)干細(xì)胞受體CXCR4和NMDAR1的蛋白偶聯(lián)情況。western印跡檢測其可能的下游信號通路。 結(jié)果電場刺激可促使大鼠胎鼠神經(jīng)干細(xì)胞表達(dá)受體CXCR4和NMDAR1逐漸增多,與電場刺激時間成正比；電場刺激還可促使兩受體發(fā)生蛋白偶聯(lián)作用。此外,電場刺激還可使PTEN和Akt的活性發(fā)生相應(yīng)改變。 結(jié)論生理強(qiáng)度的電場刺激不僅可促使大鼠胎鼠神經(jīng)干細(xì)胞表達(dá)神經(jīng)受體NMDAR1和趨化受體CXCR4上調(diào),還可誘導(dǎo)這兩種受體發(fā)生蛋白相互偶聯(lián)作用,并可能通過PTEN/PI3K/Akt信號通路的作用,介導(dǎo)神經(jīng)干細(xì)胞的定向遷移。本研究結(jié)果提示,生理強(qiáng)度的電場作用可通過對神經(jīng)遞質(zhì)受體和化學(xué)趨化受體的雙重作用,驅(qū)動神經(jīng)干細(xì)胞的定向遷移,為臨床神經(jīng)損傷修復(fù)的康復(fù)治療提供了一種新的治療策略。
[Abstract]:Objective to investigate the effects of physiological intensity electric field (250 MV / mm) stimulation on the expression and interaction of neural stem cell receptor (CXCR4) and neural stem cell receptor (NMDAR1) in rat fetal rat neural stem cells (NSCs) and its possible mechanism.Methods Neural stem cells (NSCs) from rat fetal rats were isolated and cultured in vitro and applied electric field. The expression of CXCR4 and NMDAR1 in neural stem cells (NSCs) before and after electric field stimulation was detected by Western blotting and cellular immunofluorescence chemistry.The protein coupling of neural stem cell receptor CXCR4 and NMDAR1 before and after electric field stimulation was detected by immunoprecipitation and immunofluorescence. Western blot was used to detect its possible downstream signal pathway.Results the expression of CXCR4 and NMDAR1 in rat fetal neural stem cells was increased by electric field stimulation, which was in direct proportion to the time of electric field stimulation, and the protein coupling of the two receptors was also induced by electric field stimulation.In addition, the activity of PTEN and Akt can be changed by electric field stimulation.Conclusion the electric field stimulation of physiological intensity can not only up-regulate the expression of neural receptor NMDAR1 and chemotactic receptor CXCR4 in rat fetal neural stem cells, but also induce the interaction between the two receptors, which may be mediated by the PTEN/PI3K/Akt signaling pathway.To mediate the directional migration of neural stem cells.The results suggest that the electric field action of physiological intensity can drive the directional migration of neural stem cells through the dual action of neurotransmitter receptor and chemotactic receptor.It provides a new treatment strategy for the rehabilitation of clinical nerve injury.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R363

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本文編號：1765630