本文選題：生長激素　切入點：自然殺傷細胞　出處：《山東醫(yī)藥》2017年18期 　論文類型：期刊論文

【摘要】：目的觀察生長激素(GH)對人外周血來源自然殺傷(NK)細胞殺傷活性的影響,并探討其機制。方法選取10名健康志愿者,采用免疫磁珠法分離并培養(yǎng)其外周血NK細胞。將NK細胞分為A、B、C組,分別與終濃度0、5、20μg/mL的重組人GH共培養(yǎng)48 h。以人類髓性白血病K562細胞為靶細胞,NK細胞為效應(yīng)細胞,按不同的效靶比(25∶1、50∶1、100∶1)混合,采用CCK-8法檢測NK細胞對K562細胞的殺傷率;取各組培養(yǎng)48 h的NK細胞,分別采用流式細胞術(shù)和Real-time RT-PCR法檢測NK細胞表面活化受體NKG2D蛋白及基因。結(jié)果三個不同效靶比下,B、C組NK細胞對K562細胞的殺傷率高于A組,且C組高于B組(P均0.01)。A、B、C組NKG2D陽性表達的NK細胞百分?jǐn)?shù)分別為78.8%±2.8%、87.5%±3.1%、91.8%±3.3%,B、C組NKG2D陽性表達的NK細胞百分?jǐn)?shù)高于A組(P均0.05)。A、B、C組NKG2D mRNA相對表達量分別為1±0.01、5±2.32、10±3.21,B、C組NKG2D mRNA相對表達量高于A組,且C組高于B組(P均0.05)。結(jié)論 GH可通過上調(diào)NK細胞NKG2D的表達,增強其殺傷活性。
[Abstract]:Objective to observe the effect of growth hormone (GH) on the cytotoxicity of NK cells from human peripheral blood and its mechanism. Methods Ten healthy volunteers were selected. NK cells in peripheral blood were isolated and cultured by immunomagnetic bead method. NK cells were divided into two groups: group C, co-cultured with recombinant human GH with final concentration of 0 ~ 5 渭 g/mL for 48 h. Human myeloid leukemia K562 cells were used as target cells and NK cells were used as effector cells. The cytotoxicity of NK cells to K562 cells was detected by CCK-8 assay, and the NK cells were cultured for 48 h. Flow cytometry and Real-time RT-PCR were used to detect the surface activated receptor (NKG2D) protein and gene of NK cells. Results the cytotoxicity of NK cells to K562 cells was higher in group C than in group A. The percentage of NKG2D positive NK cells in group C was higher than that in group B (78.8% 鹵2.8% 鹵3.1% 鹵3.1% 鹵3.1% 鹵3.31%), respectively. The relative expression of NKG2D mRNA in group C was higher than that in group A (1 鹵0.01 鹵2.32 10 鹵3.21). The relative expression of NKG2D mRNA in group C was higher than that in group A (P = 0.05 鹵2.32 10 鹵3.21), and the percentage of NK cells in group C was higher than that in group A (P = 0.05 鹵2.32 10 鹵3.21), and the percentage of NK cells in group C was higher than that in group A (P 0.05 鹵2.32 10 鹵3.21). Conclusion GH can enhance the cytotoxicity of NK cells by upregulating the expression of NKG2D.
【作者單位】： 濱州醫(yī)學(xué)院附屬醫(yī)院;
【基金】：國家自然科學(xué)基金資助項目(81171119) 濱州醫(yī)學(xué)院科研計劃課題項目(BY2015KJ28)
【分類號】：R392

【相似文獻】

相關(guān)期刊論文 前6條

1 肖偉玲;梁淑娟;牟東珍;王雪凈;吳慧娜;;小鼠IL-1β在H22細胞的表達及對NK細胞殺傷活性的影響[J];細胞與分子免疫學(xué)雜志;2007年12期

2 高揚,林嘉友,李莉;老化對甲硫腦啡肽增強大鼠NK細胞殺傷活性的影響(論著摘要)[J];中國神經(jīng)免疫學(xué)和神經(jīng)病學(xué)雜志;1998年01期

3 李衛(wèi)華;高金波;李鳳琴;;化學(xué)發(fā)光法測定NK細胞殺傷活性的可行性研究[J];佳木斯醫(yī)學(xué)院學(xué)報;1996年06期

4 石梅,孫朝陽,劉雪松;自體血離體照射回輸對大鼠NK細胞殺傷活性和T細胞亞群的影響[J];細胞與分子免疫學(xué)雜志;2001年04期

5 梁淑娟;肖偉玲;牟東珍;吳慧娜;王雪凈;;IL-1β反義RNA在HepG2細胞的表達及對NK細胞殺傷活性的影響[J];細胞與分子免疫學(xué)雜志;2007年08期

6 ;[J];;年期

相關(guān)碩士學(xué)位論文 前2條

1 高海燕;人CD4～+CD25～+免疫調(diào)節(jié)性T細胞對NK細胞殺傷活性的影響[D];中國人民解放軍軍事醫(yī)學(xué)科學(xué)院;2006年

2 萬瑤;P物質(zhì)與神經(jīng)生長因子對正常人外周血中NK細胞殺傷活性的影響[D];中國醫(yī)科大學(xué);2006年

，

本文編號：1647731