本文選題：大鼠　切入點(diǎn)：初級視皮層　出處：《天津醫(yī)科大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 本研究旨在從神經(jīng)元集群水平研究大鼠初級視皮層(V1區(qū))局部場電位(local field potential, LFP)振蕩的特性,探討大鼠初級視皮層的層間特異性；比較出生后第2、3周和成年大鼠V1區(qū)LFP的特性,觀察視皮層發(fā)育過程中L5(10-20Hz)頻段LFP的變化,以期闡述發(fā)育期不同階段大鼠視皮層自發(fā)LFP振蕩活動(dòng)的特性。以此來探討抑制性成分在視覺可塑性中的作用。 方法 取健康Wistar大鼠62只,根據(jù)其處于發(fā)育期的不同階段分為三組：發(fā)育關(guān)鍵期初期組(P14-16)15只,發(fā)育關(guān)鍵期高峰期組(P20-22)22只,發(fā)育成熟期組(P60)25只。應(yīng)用細(xì)胞外記錄技術(shù),記錄麻醉狀態(tài)下發(fā)育期不同階段大鼠V1區(qū)LFP振蕩活動(dòng)。利用快速傅立葉轉(zhuǎn)換(Fast Fourier Transformation,FFT)獲得功率頻譜圖(Power Spectrum),分析各組V1區(qū)2+3層(L2/3)和第5層(L5)低頻段和(10-20Hz)高頻段的功率成分(Power)。根據(jù)公式：功率比(Power Ratio)=(P high+Plow)/(P high-Plow),求得各個(gè)記錄位點(diǎn)的Power Ratio,并繪制Power Ratio與皮層深度分布散點(diǎn)圖。分別比較三組間L5(10-20Hz)頻段Power和Power Ratio差異,繪制簡單條形圖。觀察大鼠V1區(qū)LFP的層間差異在不同發(fā)育期的變化規(guī)律。 結(jié)果 1.發(fā)育關(guān)鍵期初期大鼠V1區(qū)LFP振蕩特性 采集到P14-16大鼠V1區(qū)28個(gè)記錄位點(diǎn)自發(fā)鋒電位反應(yīng)良好的LFP信號。該組大鼠V1區(qū)LFP振蕩活動(dòng)表現(xiàn)出層間差異。L2/3以(2-5Hz)低頻振蕩為主,L5除了(2-5)Hz頻段存在Power峰值以外,在(10-20)Hz頻段也出現(xiàn)了強(qiáng)烈的振蕩活動(dòng)。Power Ratio與皮層深度相關(guān)性分析顯示：兩者呈正相關(guān)(r=0.527,P=0.007)有統(tǒng)計(jì)學(xué)意義,回歸方程為：y=-1.293+0.001x�？焖僬袷�(高Power Ratio)主要集中在深層：L5Power Ratio集中在-0.3-0.6之間,而L2/3則小于-0.3。 2.發(fā)育關(guān)鍵期高峰期大鼠V1區(qū)LFP振蕩特性 采集到P20-22大鼠V1區(qū)25個(gè)記錄位點(diǎn)自發(fā)鋒電位反應(yīng)良好的LFP信號。其自發(fā)LFP未觀察到層間差異,記錄各層都以(2-5)Hz低頻振蕩為主。對皮層深度與Power Ratio繪制散點(diǎn)圖并進(jìn)行相關(guān)分析,結(jié)果顯示兩者沒有明顯相關(guān)性(r=0.07,P=0.733)。 3.發(fā)育成熟期大鼠V1區(qū)LFP振蕩特性 采集到P60大鼠V1區(qū)24個(gè)記錄位點(diǎn)自發(fā)鋒電位反應(yīng)良好的LFP信號。該層自發(fā)LFP振蕩表現(xiàn)與關(guān)鍵期初期組相似存在層間差異,L2/3以(2-5Hz)低頻振蕩為主,L5除了低頻段功率頻譜存在高峰以外,在(10-20Hz)高頻段也有較強(qiáng)的振蕩活動(dòng)。對皮層深度與Power Ratio繪制散點(diǎn)圖并進(jìn)行相關(guān)分析,結(jié)果顯示兩者呈正相關(guān)(r=0.663,P=0.000)有統(tǒng)計(jì)學(xué)意義。其回歸方程為：y=-0.649+0.001x。 4.發(fā)育期不同階段V1區(qū)LFP特性的比較 采集到三組V1區(qū)77個(gè)記錄位點(diǎn)自發(fā)鋒電位反應(yīng)良好的LFP信號。三組V1區(qū)L5自發(fā)LFP的Power值和Power Ratio值分別進(jìn)行單因素方差分析。統(tǒng)計(jì)結(jié)果顯示：關(guān)鍵期初期組和成年組L5Power相比差異無統(tǒng)計(jì)學(xué)意義(P=0.828),兩組Power Ratio相比差異也無統(tǒng)計(jì)學(xué)意義(P=0.551)。而關(guān)鍵期高峰組和其他兩組比較差異均有統(tǒng)計(jì)學(xué)意義(P=0.000)。 結(jié)論 1.發(fā)育關(guān)鍵期初期和成熟期大鼠V1區(qū)的自發(fā)局部場電位振蕩存在層間差異。 2.V1區(qū)第5層高頻段LFP振蕩活動(dòng)在發(fā)育期不同階段表現(xiàn)不同。 3.實(shí)驗(yàn)結(jié)果與發(fā)育關(guān)鍵期GABA能理論吻合,支持抑制性成分在視皮層發(fā)育可塑性關(guān)鍵期中的作用。
[Abstract]:objective
The purpose of this study is to research from the cluster level of neurons in the rat primary visual cortex (area V1) local field potential (local field potential, LFP) oscillation characteristics of the primary visual cortex of rats between the layers of specificity; characteristics of 2,3 week and LFP V1 adult rats compared after birth, the development of visual observation in the cortex of L5 (10-20Hz) LFP to change frequency, this development stage characteristics of visual cortex LFP spontaneous oscillatory activity in different stages of rats. In order to investigate the inhibitory component of visual plasticity in the role.
Method
62 healthy Wistar rats at different stages in the developmental stages were divided into three groups: the early development of the critical period group (P14-16) 15, the critical period of growth peak group (P20-22) 22, mature stage group (P60) 25. Application of extracellular recording technique to record under anesthesia state development at different stages of rat V1 LFP oscillatory activity. Conversion by fast Fu Liye (Fast Fourier Transformation, FFT) power spectrum (Power Spectrum), V1 was analyzed 2+3 layer (L2/3) and fifth (L5) and low frequency (10-20Hz) power frequency components (Power). According to the formula: power ratio (Power Ratio) = (P / high+Plow) (P high-Plow), to achieve the various recording sites of Power Ratio and Power Ratio and cortex depth distribution of scatter diagram drawing. Were compared between the three groups (L5 10-20Hz) and Power Ratio Power frequency difference, draw a simple bar chart. Observe the LFP V1 region in the layer The variation of the difference between different developmental stages.
Result
1. LFP oscillation in V1 region of rats at the early stage of development
Collected 28 recording sites of P14-16 in V1 area of rats with spontaneous LFP spike signal response is good. The rats in group V1 LFP showed oscillatory activity difference between layers of.L2/3 (2-5Hz) with low frequency oscillation, in addition to L5 (2-5) Hz band Power peak, at (10-20) Hz band also appeared the correlation between.Power Ratio and cortical oscillation depth strongly analysis showed that they were positively correlated (r=0.527, P=0.007) were statistically significant, the regression equation is: y=-1.293+0.001x. fast oscillation (high Power Ratio) mainly concentrated in the deep L5Power Ratio concentrated in -0.3-0.6, while the L2/3 is less than -0.3.
2. LFP oscillation in V1 region of rats during peak period of developmental critical period
Collected 25 recording sites of P20-22 in V1 area of rats with spontaneous LFP spike signal response is good. The spontaneous LFP no observed difference between layers, each layer to record (2-5) Hz of low frequency oscillation. The cortical depth and Power Ratio scatter diagram and correlation analysis, results showed that there was no significant correlation between the two researchers (r=0.07, P=0.733).
3. LFP oscillation in V1 region of mature rats
Collected 24 recording sites of P60 in V1 area of rats with spontaneous LFP spike signal response is good. The LFP layer and the critical period of spontaneous oscillation period were similar to the existing difference between layers, L2/3 (2-5Hz) with low frequency oscillation, L5 low frequency power spectrum peak in addition to outside, in the high frequency (10-20Hz) is oscillatory activity is strong. The cortical depth and Power Ratio scatter diagram and correlation analysis showed a positive correlation (r=0.663, P=0.000) were statistically significant. The regression equation is: y=-0.649+0.001x.
Comparison of LFP characteristics in V1 region at different stages of development stage 4.
The collected 77 records were three V1 in group LFP spontaneous spike signal response is good. The three group of V1 L5 and Power LFP spontaneous Power value Ratio value respectively by single factor variance analysis. The statistical results show that the critical period of the early group and adult group L5Power had no significant difference between the two groups (P= 0.828). Power Ratio compared with no significant differences (P=0.551). And the peak of critical period group and other two groups were statistically significant (P=0.000).
conclusion
1. the spontaneous local field potential oscillations in the V1 region of the early and mature stage of the developmental critical period exist in the interlayer difference.
The LFP oscillations in the fifth layer of high frequency section of the 2.V1 region are different at different stages of development.
3. the results of the experiment coincide with the theory of GABA at the critical stage of development, supporting the role of the inhibitory components in the plastic critical stage of the development of the visual cortex.

【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R339.141

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相關(guān)期刊論文 前1條

1 李東升;谷宇;王立;高江遠(yuǎn);石建;;GABA能聯(lián)系對大鼠初級視皮層Ⅱ/Ⅲ層神經(jīng)元突觸可塑性的影響[J];復(fù)旦學(xué)報(bào)(自然科學(xué)版);2010年05期

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本文編號：1604046