本文選題：母嬰分離　切入點：內臟高敏感　出處：《福建醫(yī)科大學》2012年碩士論文　論文類型：學位論文

【摘要】：目的：采用母嬰分離大鼠，觀察大鼠背根神經節(jié)中瞬時感受器電位錨蛋白-1(TRPA1)表達變化，及其與內臟感覺敏感性、脊髓背角降鈣素基因相關肽(CGRP)和P物質(SP)表達水平的關系，探討TRPA1在母嬰分離大鼠內臟高敏感性發(fā)生中的作用。 方法：按析因設計，將32只新生雄性SD大鼠隨機分為4組，每組8只。A1B1組：正常飼養(yǎng)+結直腸擴張(CRD)前腹腔注射生理鹽水；A1B2組：正常飼養(yǎng)+CRD前腹腔注射釕紅；A2B1組：母嬰分離+CRD前腹腔注射生理鹽水；A2B2組，母嬰分離+CRD前腹腔注射釕紅。大鼠成年后進行CRD來觀察腹壁撤退反射(AWR)和腹外斜肌肌電(EEOA)的檢測，取遠端結腸組織進行HE檢測，并用免疫組化觀察L6-S1節(jié)段背根神經節(jié)中的TRPA1和相應脊髓背角中CGRP、SP的表達。 結果： (1)母嬰分離可以使大鼠受CRD刺激時AWR評分升高，，母嬰分離對AWR評分影響的主效應在CRD壓力40~80mmHg時，均有統(tǒng)計學意義（p值分別為0.036、0.009、0.027）。CRD前腹腔注射釕紅可以降低AWR評分，釕紅對AWR評分影響的主效應在CRD壓力40~60mmHg時，有統(tǒng)計學意義（p值分別為0.036、0.039）。CRD壓力20~80mmHg時母嬰分離和釕紅對AWR評分的影響無顯著性交互作用(p值分別為0.361、0.128、0.352、0.130)； (2)母嬰分離可以使大鼠在受CRD刺激時EEOA值升高，主效應在CRD壓力為40~80mmHg時，均有統(tǒng)計學意義（p值分別為0.000、0.000、0.000）。CRD前腹腔注射釕紅可降低EEOA值，主效應在60~80mmHg時，均有統(tǒng)計學意義（p值分別為0.003、0.039）。CRD壓力20~80mmHg時母嬰分離和釕紅對EEOA值的影響無顯著性交互作用(p值分別為0.744、0.931、0.062、0.572)； (3)母嬰分離可以使TRPA1和CGRP、SP的平均光密度值升高，其主效應均有統(tǒng)計學意義（p值分別為0.001、0.000、0.000）。CRD前腹腔注射釕紅對TRPA1和CGRP、SP的平均光密度值影響的主效應均無統(tǒng)計學意義（p值分別為0.186、0.509、0.579）。母嬰分離和釕紅對TRPA1和CGRP、SP的影響均無顯著性交互作用(p值分別0.321、0.936、0.777)； (4)TRPA1與CGRP、SP、AWR評分、EEOA值之間分別存在正相關關系（p均0.05）。 結論：新生期母嬰分離可引起大鼠內臟高敏感性，背根神經節(jié)TRPAl參與內臟高敏感性的形成。
[Abstract]:Objective: to observe the changes of transient receptor potential anchor protein (-1TRPA1) expression in rat dorsal root ganglion (DRG) and its relationship with visceral sensibility, calcitonin gene-related peptide (CGRPP) and substance P substance (SP) expression in dorsal horn of rats. To investigate the role of TRPA1 in the development of visceral hypersensitivity in rats with maternal and infant separation. Methods: according to the factorial design, 32 newborn male SD rats were randomly divided into 4 groups. Eight rats in each group were treated by intraperitoneal injection of saline A1B2 before normal colorectal dilatation. Group A 2B1 was injected intraperitoneally before normal feeding of CRD: group A 2B2 was injected with normal saline before CRD. Ruthenium red was injected intraperitoneally before mother-to-child separation (CRD). CRD was used to observe the abdominal wall withdrawal reflex (CRD) and the external oblique muscle electromyography (EEOAA), and the distal colon tissue was taken for HE detection. The expression of TRPA1 in L6-S1 segment dorsal root ganglion and the expression of CGRP PSP in the corresponding dorsal horn of spinal cord were observed by immunohistochemistry. Results:. 1) Maternal and infant separation could increase the AWR score of rats stimulated by CRD. The main effect of maternal and infant separation on AWR score was 0.036 鹵0.009 ~ 0.027 鹵0.027. The main effect of maternal and infant separation on AWR score was 0.036 鹵0.009 ~ 0.027 mm Hg, respectively. The AWR score could be decreased by intraperitoneal injection of ruthenium red. The main effect of ruthenium red on AWR score was 0.036 鹵0.039 鹵0.039 mm Hg for CRD pressure and 0.036 鹵0.039 mm Hg for CRD pressure, respectively, and there was no significant interaction between Ru red and Ru Red on AWR score (P = 0.361) 0.1280.3520.130 mm Hg, respectively. (2) Maternal and infant separation could increase the EEOA value of rats stimulated by CRD, and the main effect was that when the CRD pressure was 40,80mmHg, there was significant difference in the main effect between the two groups. The value of Ru red could be decreased by intraperitoneal injection of ruthenium red before CRD, and the main effect was at 60 ~ 80mmHg. There was no significant interaction between maternal and infant separation and ruthenium red on EEOA value at the pressure of 20 ~ 80mmHg (P = 0.003 ~ 0.039), P = 0.744 ~ (0.931) ~ (0.062) ~ (2) ~ (0.572g), P = 0.74 4 ~ (-1) ~ 0. 931 ~ 0. 062 鹵0. 572 mm Hg, respectively. 3) the average optical density of TRPA1 and CGRP SP was increased by maternal separation. There was no significant difference in the main effects of Ru red on the average optical density of TRPA1 and CGRP SP before the injection of ruthenium red. The main effects were not statistically significant. The effects of maternal and infant separation and ruthenium red on TRPA1 and CGRPSP were not significant, respectively. There was no significant difference in the effects of Ru red on TRPA1 and CGRPSP before CRD. The main effects of Ru on TRPA1 and CGRPSP were not statistically significant (P = 0. 186, P = 0. 509 / 0. 579, respectively). The P value of significant interaction was 0. 321 鹵0. 936 and 0. 777m, respectively. There was a positive correlation between TRPA1 and SPAWR score and EEOA value. Conclusion: neonatal maternal and infant separation can induce visceral hypersensitivity in rats. TRPAl in dorsal root ganglion is involved in the formation of visceral hypersensitivity.
【學位授予單位】：福建醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R363

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