本文選題：母嬰分離　切入點(diǎn)：內(nèi)臟高敏感　出處：《福建醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：采用母嬰分離大鼠，觀察大鼠背根神經(jīng)節(jié)中瞬時(shí)感受器電位錨蛋白-1(TRPA1)表達(dá)變化，及其與內(nèi)臟感覺(jué)敏感性、脊髓背角降鈣素基因相關(guān)肽(CGRP)和P物質(zhì)(SP)表達(dá)水平的關(guān)系，探討TRPA1在母嬰分離大鼠內(nèi)臟高敏感性發(fā)生中的作用。 方法：按析因設(shè)計(jì)，將32只新生雄性SD大鼠隨機(jī)分為4組，每組8只。A1B1組：正常飼養(yǎng)+結(jié)直腸擴(kuò)張(CRD)前腹腔注射生理鹽水；A1B2組：正常飼養(yǎng)+CRD前腹腔注射釕紅；A2B1組：母嬰分離+CRD前腹腔注射生理鹽水；A2B2組，母嬰分離+CRD前腹腔注射釕紅。大鼠成年后進(jìn)行CRD來(lái)觀察腹壁撤退反射(AWR)和腹外斜肌肌電(EEOA)的檢測(cè)，取遠(yuǎn)端結(jié)腸組織進(jìn)行HE檢測(cè)，并用免疫組化觀察L6-S1節(jié)段背根神經(jīng)節(jié)中的TRPA1和相應(yīng)脊髓背角中CGRP、SP的表達(dá)。 結(jié)果： (1)母嬰分離可以使大鼠受CRD刺激時(shí)AWR評(píng)分升高，，母嬰分離對(duì)AWR評(píng)分影響的主效應(yīng)在CRD壓力40~80mmHg時(shí)，均有統(tǒng)計(jì)學(xué)意義（p值分別為0.036、0.009、0.027）。CRD前腹腔注射釕紅可以降低AWR評(píng)分，釕紅對(duì)AWR評(píng)分影響的主效應(yīng)在CRD壓力40~60mmHg時(shí)，有統(tǒng)計(jì)學(xué)意義（p值分別為0.036、0.039）。CRD壓力20~80mmHg時(shí)母嬰分離和釕紅對(duì)AWR評(píng)分的影響無(wú)顯著性交互作用(p值分別為0.361、0.128、0.352、0.130)； (2)母嬰分離可以使大鼠在受CRD刺激時(shí)EEOA值升高，主效應(yīng)在CRD壓力為40~80mmHg時(shí)，均有統(tǒng)計(jì)學(xué)意義（p值分別為0.000、0.000、0.000）。CRD前腹腔注射釕紅可降低EEOA值，主效應(yīng)在60~80mmHg時(shí)，均有統(tǒng)計(jì)學(xué)意義（p值分別為0.003、0.039）。CRD壓力20~80mmHg時(shí)母嬰分離和釕紅對(duì)EEOA值的影響無(wú)顯著性交互作用(p值分別為0.744、0.931、0.062、0.572)； (3)母嬰分離可以使TRPA1和CGRP、SP的平均光密度值升高，其主效應(yīng)均有統(tǒng)計(jì)學(xué)意義（p值分別為0.001、0.000、0.000）。CRD前腹腔注射釕紅對(duì)TRPA1和CGRP、SP的平均光密度值影響的主效應(yīng)均無(wú)統(tǒng)計(jì)學(xué)意義（p值分別為0.186、0.509、0.579）。母嬰分離和釕紅對(duì)TRPA1和CGRP、SP的影響均無(wú)顯著性交互作用(p值分別0.321、0.936、0.777)； (4)TRPA1與CGRP、SP、AWR評(píng)分、EEOA值之間分別存在正相關(guān)關(guān)系（p均0.05）。 結(jié)論：新生期母嬰分離可引起大鼠內(nèi)臟高敏感性，背根神經(jīng)節(jié)TRPAl參與內(nèi)臟高敏感性的形成。
[Abstract]:Objective: to observe the changes of transient receptor potential anchor protein (-1TRPA1) expression in rat dorsal root ganglion (DRG) and its relationship with visceral sensibility, calcitonin gene-related peptide (CGRPP) and substance P substance (SP) expression in dorsal horn of rats. To investigate the role of TRPA1 in the development of visceral hypersensitivity in rats with maternal and infant separation. Methods: according to the factorial design, 32 newborn male SD rats were randomly divided into 4 groups. Eight rats in each group were treated by intraperitoneal injection of saline A1B2 before normal colorectal dilatation. Group A 2B1 was injected intraperitoneally before normal feeding of CRD: group A 2B2 was injected with normal saline before CRD. Ruthenium red was injected intraperitoneally before mother-to-child separation (CRD). CRD was used to observe the abdominal wall withdrawal reflex (CRD) and the external oblique muscle electromyography (EEOAA), and the distal colon tissue was taken for HE detection. The expression of TRPA1 in L6-S1 segment dorsal root ganglion and the expression of CGRP PSP in the corresponding dorsal horn of spinal cord were observed by immunohistochemistry. Results:. 1) Maternal and infant separation could increase the AWR score of rats stimulated by CRD. The main effect of maternal and infant separation on AWR score was 0.036 鹵0.009 ~ 0.027 鹵0.027. The main effect of maternal and infant separation on AWR score was 0.036 鹵0.009 ~ 0.027 mm Hg, respectively. The AWR score could be decreased by intraperitoneal injection of ruthenium red. The main effect of ruthenium red on AWR score was 0.036 鹵0.039 鹵0.039 mm Hg for CRD pressure and 0.036 鹵0.039 mm Hg for CRD pressure, respectively, and there was no significant interaction between Ru red and Ru Red on AWR score (P = 0.361) 0.1280.3520.130 mm Hg, respectively. (2) Maternal and infant separation could increase the EEOA value of rats stimulated by CRD, and the main effect was that when the CRD pressure was 40,80mmHg, there was significant difference in the main effect between the two groups. The value of Ru red could be decreased by intraperitoneal injection of ruthenium red before CRD, and the main effect was at 60 ~ 80mmHg. There was no significant interaction between maternal and infant separation and ruthenium red on EEOA value at the pressure of 20 ~ 80mmHg (P = 0.003 ~ 0.039), P = 0.744 ~ (0.931) ~ (0.062) ~ (2) ~ (0.572g), P = 0.74 4 ~ (-1) ~ 0. 931 ~ 0. 062 鹵0. 572 mm Hg, respectively. 3) the average optical density of TRPA1 and CGRP SP was increased by maternal separation. There was no significant difference in the main effects of Ru red on the average optical density of TRPA1 and CGRP SP before the injection of ruthenium red. The main effects were not statistically significant. The effects of maternal and infant separation and ruthenium red on TRPA1 and CGRPSP were not significant, respectively. There was no significant difference in the effects of Ru red on TRPA1 and CGRPSP before CRD. The main effects of Ru on TRPA1 and CGRPSP were not statistically significant (P = 0. 186, P = 0. 509 / 0. 579, respectively). The P value of significant interaction was 0. 321 鹵0. 936 and 0. 777m, respectively. There was a positive correlation between TRPA1 and SPAWR score and EEOA value. Conclusion: neonatal maternal and infant separation can induce visceral hypersensitivity in rats. TRPAl in dorsal root ganglion is involved in the formation of visceral hypersensitivity.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 林濱榕;吳斌;卓玲;陳競(jìng)芳;張睿;;幼鼠慢性內(nèi)臟高敏感模型的建立及評(píng)價(jià)[J];福建醫(yī)科大學(xué)學(xué)報(bào);2010年02期

2 蔡琴燕;唐影;黃楊;祝福存;林春;;動(dòng)物麻醉機(jī)在腸易激綜合征模型大鼠評(píng)價(jià)中的應(yīng)用[J];福建醫(yī)科大學(xué)學(xué)報(bào);2011年01期

3 劉海燕;;灌注固定大鼠的技術(shù)操作及常見(jiàn)問(wèn)題[J];齊齊哈爾醫(yī)學(xué)院學(xué)報(bào);2006年11期

4 林濱榕;吳斌;卓玲;陳兢芳;楊燕珍;張睿;;新生期母嬰分離對(duì)幼鼠內(nèi)臟痛敏感性及脊髓Fos蛋白表達(dá)的影響[J];中國(guó)新生兒科雜志;2010年02期

5 王承黨;鄭雪雁;鄭瑋瑋;;結(jié)腸黏膜低度炎癥對(duì)大鼠內(nèi)臟感覺(jué)的影響[J];世界華人消化雜志;2009年16期

6 王亞雷;姚瑋艷;章永平;喬敏敏;袁耀宗;;阻斷Nav1．8表達(dá)對(duì)大鼠內(nèi)臟高敏感性影響的研究[J];中華消化雜志;2006年02期

相關(guān)碩士學(xué)位論文 前1條

1 林劍;早期應(yīng)激對(duì)SD大鼠內(nèi)臟感覺(jué)和腸道炎癥的影響[D];福建醫(yī)科大學(xué);2011年

本文編號(hào)：1588493