本文選題：IDO　切入點：大鼠骨髓間充質(zhì)干細胞　出處：《中國免疫學(xué)雜志》2017年10期 　論文類型：期刊論文

【摘要】：目的:探討過表達IDO大鼠骨髓間充質(zhì)干細胞(BMSC)改善大鼠腹腔異位移植心臟的存活機制。方法:通過慢病毒載體GV308攜帶IDO基因轉(zhuǎn)染大鼠骨髓間充質(zhì)干細胞構(gòu)建過表達IDO大鼠骨髓間充質(zhì)干細胞。建立大鼠腹腔異位移植心臟模型,經(jīng)尾靜脈給予相應(yīng)細胞處理:(1)利用超聲心動圖檢測移植心臟心功能變化。(2)采用小動物活體成像系統(tǒng)評估移植心臟局部熒光強度。(3)再取各組受體大鼠的脾臟,采用流式細胞技術(shù)檢測CD40、CD86、CD80、MHCⅡ、CD274、CD45RA、CD45RA+CD45RB、Treg細胞的表達情況。(4)取各組移植心臟,采用HE染色評估炎性細胞浸潤情況。(5)利用液相芯片檢測各組血清IL-1ɑ、IL-4、IL-1β、IL-2、IL-10、IFN-γ、IL-18、TGFβ1、TGFβ2、TGFβ3因子的變化情況。結(jié)果:(1)給予相應(yīng)細胞處理,可見過表達IDO-BMSCs處理后2 d移植心臟的EF、FS較其余各組有提高。(2)采用小動物活體評估可見過表達IDO-BMSCs組移植心臟局部熒光強度最強。(3)給予干預(yù)措施后2 d可見過表達IDO-BMSCs組脾臟細胞CD40、CD86、CD80、MHCⅡ、CD45RA、CD45RA+CD45RB表達降低,而CD274、Treg細胞的表達增高。(4)采用液相芯片檢測各組提取血清可見在2 d時,過表達IDOBMSCs組血清中IL-1α、IL-4、IL-1β、IL-2、IFN-γ、IL-18是降低的;IL-10、TGFβ1、TGFβ2、TGFβ3表達量是升高的。HE染色證實過表達IDO-BMSCs組炎性細胞浸潤少于其他組。結(jié)論:過表達IDO的BMSCs可以通過有效調(diào)節(jié)免疫DC、T細胞及細胞因子,從多個免疫層面改善移植心臟存活。
[Abstract]:Objective: to investigate the survival mechanism of IDO rat bone marrow mesenchymal stem cells (BMSCs) in improving the survival of rat heart allograft. Methods: Lentivirus vector GV308 carrying IDO gene was used to transfect rat bone marrow mesenchymal stem cells (BMSCs). Bone marrow mesenchymal stem cells (BMSCs) of IDO rats were used to establish an ectopic heart transplantation model. The changes of cardiac function were detected by echocardiography) the local fluorescence intensity of the transplanted heart was evaluated by small animal in vivo imaging system, and then the spleen of each group of recipient rats was taken out. Flow cytometry was used to detect the expression of CD40, CD86, CD80, MHC 鈪，

本文編號：1588472