本文選題：IDO　切入點(diǎn)：大鼠骨髓間充質(zhì)干細(xì)胞　出處：《中國(guó)免疫學(xué)雜志》2017年10期 　論文類型：期刊論文

【摘要】：目的:探討過(guò)表達(dá)IDO大鼠骨髓間充質(zhì)干細(xì)胞(BMSC)改善大鼠腹腔異位移植心臟的存活機(jī)制。方法:通過(guò)慢病毒載體GV308攜帶IDO基因轉(zhuǎn)染大鼠骨髓間充質(zhì)干細(xì)胞構(gòu)建過(guò)表達(dá)IDO大鼠骨髓間充質(zhì)干細(xì)胞。建立大鼠腹腔異位移植心臟模型,經(jīng)尾靜脈給予相應(yīng)細(xì)胞處理:(1)利用超聲心動(dòng)圖檢測(cè)移植心臟心功能變化。(2)采用小動(dòng)物活體成像系統(tǒng)評(píng)估移植心臟局部熒光強(qiáng)度。(3)再取各組受體大鼠的脾臟,采用流式細(xì)胞技術(shù)檢測(cè)CD40、CD86、CD80、MHCⅡ、CD274、CD45RA、CD45RA+CD45RB、Treg細(xì)胞的表達(dá)情況。(4)取各組移植心臟,采用HE染色評(píng)估炎性細(xì)胞浸潤(rùn)情況。(5)利用液相芯片檢測(cè)各組血清IL-1ɑ、IL-4、IL-1β、IL-2、IL-10、IFN-γ、IL-18、TGFβ1、TGFβ2、TGFβ3因子的變化情況。結(jié)果:(1)給予相應(yīng)細(xì)胞處理,可見(jiàn)過(guò)表達(dá)IDO-BMSCs處理后2 d移植心臟的EF、FS較其余各組有提高。(2)采用小動(dòng)物活體評(píng)估可見(jiàn)過(guò)表達(dá)IDO-BMSCs組移植心臟局部熒光強(qiáng)度最強(qiáng)。(3)給予干預(yù)措施后2 d可見(jiàn)過(guò)表達(dá)IDO-BMSCs組脾臟細(xì)胞CD40、CD86、CD80、MHCⅡ、CD45RA、CD45RA+CD45RB表達(dá)降低,而CD274、Treg細(xì)胞的表達(dá)增高。(4)采用液相芯片檢測(cè)各組提取血清可見(jiàn)在2 d時(shí),過(guò)表達(dá)IDOBMSCs組血清中IL-1α、IL-4、IL-1β、IL-2、IFN-γ、IL-18是降低的;IL-10、TGFβ1、TGFβ2、TGFβ3表達(dá)量是升高的。HE染色證實(shí)過(guò)表達(dá)IDO-BMSCs組炎性細(xì)胞浸潤(rùn)少于其他組。結(jié)論:過(guò)表達(dá)IDO的BMSCs可以通過(guò)有效調(diào)節(jié)免疫DC、T細(xì)胞及細(xì)胞因子,從多個(gè)免疫層面改善移植心臟存活。
[Abstract]:Objective: to investigate the survival mechanism of IDO rat bone marrow mesenchymal stem cells (BMSCs) in improving the survival of rat heart allograft. Methods: Lentivirus vector GV308 carrying IDO gene was used to transfect rat bone marrow mesenchymal stem cells (BMSCs). Bone marrow mesenchymal stem cells (BMSCs) of IDO rats were used to establish an ectopic heart transplantation model. The changes of cardiac function were detected by echocardiography) the local fluorescence intensity of the transplanted heart was evaluated by small animal in vivo imaging system, and then the spleen of each group of recipient rats was taken out. Flow cytometry was used to detect the expression of CD40, CD86, CD80, MHC 鈪，

本文編號(hào)：1588472