本文選題：益生菌L.casei　切入點：Zhang　出處：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：本文旨在研究益生菌L.casei Zhang對內(nèi)毒素性肝損傷大鼠的保護作用。造模方法：禁食12小時后,對大鼠腹腔注射脂多糖(LPS:50mg/kg)和D-半乳糖胺(D-GalN:300mg/kg)。(1)將大鼠分為四組：正常對照組A(n=16)；模型組B(n=16),灌服生理鹽水1m1/d,30d后造模；預(yù)防組C(n=20),灌服益生菌Lcasei Zhang1×109CFU/d,30d后造模；地塞米松組D(n=10),腹腔注射地塞米松、LPS和D-GalN,觀察15d內(nèi)大鼠的存活率,以此制備生存曲線；(2)取48只大鼠分為CTL、L/G、L/G+LCZ. L/G+DXM4組,分別作如下處理：CTL組(正常對照組)正常喂養(yǎng)、L/G組(模型組)灌胃生理鹽水1m1/d,L/G+LCZ組(預(yù)防組)灌胃益生菌菌粉懸液2×109CFU/d, L/G+DXM組(地塞米松組)腹腔注射地塞米松(10m/gkg),各組飼喂基礎(chǔ)飼料,31d后禁食12h, B、C、D三組腹腔注射LPS(50mg/kg)和D-GalN(300mg/kg),處理后16小時處死大鼠,測血漿丙氨酸轉(zhuǎn)氨酶(ALT)、天冬氨酸轉(zhuǎn)氨酶(AST)及肝臟組織中腫瘤壞死因子-a(TNF-a)、Toll受體4(TLR4)、過氧化物酶體增殖物激活受體-γ(PPARγ)、NO等細胞因子的含量,同時,測定肝臟組織中磷酸化核因子抑制蛋白(p-IκB)、PPARY、TLR4等蛋白的表達情況,此外,還觀察肝臟切片病理學(xué)變化。結(jié)果發(fā)現(xiàn)：(1)生存曲線中,C組的生存率低于A組和D組,但明顯高于B組,說明益生菌L.casei Zhang可以在一定程度上保護大鼠肝臟,減輕肝臟損傷對大鼠機體造成的損害；(2)L/G+LCZ組和L/G+DXM組的ALT、AST及肝臟組織中TNF-a、NO、TLR4、p-IκB的表達量顯著低于L/G組(P0.05),L/G+LCZ組PPARy表達高于L/GL/G組(P0.05),；L/G+LCZ組和L/G+DXM組肝臟MDA含量顯著低于L/G組(P0.05),SOD活力顯著高于L/G組(P0.05)；病理切片結(jié)果發(fā)現(xiàn),益生菌干預(yù)治療可緩減內(nèi)毒素引起的急性肝細胞損傷。因此,益生菌L.casei Zhang對內(nèi)毒素性肝損傷的治療具有良好的保護作用,可用于功能性食品的開發(fā)。
[Abstract]:The aim of this study was to study the protective effect of probiotic L.casei Zhang on endotoxin induced liver injury in rats. Rats were divided into four groups by intraperitoneal injection of lipopolysaccharide (LPS: 50 mg / kg) and D-galactosamine (D-GalN: 300mg 路kg 路kg -1). In the dexamethasone group, the lipopolysaccharide (LPS) and D-GalN were injected intraperitoneally to observe the survival rate of rats within 15 days, and the survival curve was prepared. 48 rats were divided into two groups: CTLL / GG / L / G LCZL / G DXM4 group. They were treated as follows: control group (normal control group), normal feeding group (model group), normal saline 1ml / dL / G LCZ group (prevention group), probiotic powder suspension 2 脳 109 CFU / d and dexamethasone DXM group (dexamethasone group) by intraperitoneal injection of dexamethasone 10 mg 路kg ~ (-1) 路kg ~ (-1), the control group (n = 10) was given intraperitoneal injection of dexamethasone 10 mg / g 路kg ~ (-1). The rats in each group were fed with basic feed for 31 days and fasted for 12 hours. The rats in group B were injected intraperitoneally with LPSN 50 mg / kg and D-GalN 300 mg / kg. The rats were killed 16 hours after treatment. The levels of plasma alanine aminotransferase (alt), aspartate aminotransferase (AST), tumor necrosis factor (TNF- 偽), TLR4, peroxisome proliferator activator receptor-緯 -PPAR 緯 no in liver tissue were measured. The expression of phosphorylated nuclear factor inhibitor protein (p-I 魏 B) PPARYTLR4 in liver tissue was determined. In addition, the pathological changes of liver sections were observed. It was found that the survival rate of group C was lower than that of group A and group D, but significantly higher than that of group B in the survival curve. The results showed that probiotic L.casei Zhang could protect the liver of rats to some extent. The expression of TLR4p-I 魏 B in liver tissue of rats with reduced liver injury was significantly lower than that in L- P / L / G LCZ group and L / G DXM group. The expression of MDA in liver tissue was significantly lower than that in L / G group (P 0.05% L / G LCZ group) compared with that in L / G group (P 0.05% G LCZ group) and LG DXM group (P < 0.05 P / P / G DXM group) compared with that in L / G group (P < 0.05% P < 0.05 P / L / G LCZ group). The expression of MDA in liver tissue was significantly lower than that in L / G group (P < 0.05 L / G LCZ group). The activity of SOD in P0. 05 was significantly higher than that in L- / G group. Probiotics intervention can reduce the acute hepatocyte injury induced by endotoxin. Therefore, the probiotic L.casei Zhang has a good protective effect on endotoxin induced liver injury and can be used in the development of functional food.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R371

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