本文選題：桶狀皮層　切入點：跨感覺可塑性　出處：《蚌埠醫(yī)學(xué)院》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：本研究旨在探討小鼠嗅覺損傷后觸覺跨模式可塑性桶狀皮層的細胞機理，尤其是比較長短觸須觸覺代表區(qū)A5-E1和C9-E4內(nèi)錐體神經(jīng)元可塑性的差異。 方法：1.行為學(xué)實驗：選用出生后12天的小鼠，建立嗅覺剝奪誘導(dǎo)觸覺功能上調(diào)的動物模型，實驗分為兩組，正常組和剝奪組。嗅覺剝奪后1周到2周，應(yīng)用數(shù)碼照相機對小鼠行為學(xué)進行視頻拍攝，然后在視頻軟件中記錄單位時間內(nèi)（30s）長、短觸須擺動的次數(shù)(頻率)及觸須屈曲的時間。 2.膜片鉗電生理實驗：在嗅覺剝奪后1周，用異氟烷吸入麻醉，迅速將大腦分離，將大腦桶狀皮層分為與短觸須對應(yīng)的C9-E4區(qū)和與長觸須對應(yīng)的A5-E1區(qū)，然后將大腦分離并做冠狀切片(400μm)，，分別將C9-E4區(qū)和A5-E1區(qū)的腦片放置于氧合的人工腦脊液(ASCF)中，25C人工腦脊液中，孵育1-2小時后轉(zhuǎn)移至灌流槽內(nèi)，在31C下對腦片進行灌流；采用IR-DIC光學(xué)顯微鏡(Nikon E600FN)熒光顯微鏡定位大腦桶狀皮層錐體神經(jīng)元；運用Axoclamp-200B放大器全細胞記錄大腦桶狀皮層錐體神經(jīng)元的輸入輸出曲線和桶狀皮層C9-E4區(qū)和A5-E1區(qū)錐體神經(jīng)元動作電位的閾電位(threshold potentials,Vts)、峰電位間距，信號輸入Clampex10.2軟件獲取群集發(fā)放的動作電位間距(inter-spike interval,ISI)，使用Clampfit10.2軟件進行數(shù)據(jù)分析。 結(jié)果：1.嗅覺剝奪小鼠觸須較正常組擺動頻率增加，剝奪組的長觸須擺動頻率較短觸須更顯著(P0.05)；2.應(yīng)用膜片鉗電生理方法，較正常組相比，剝奪組桶狀皮層錐體神經(jīng)元發(fā)放功能增強(P0.05)。尤其是在長觸須代表區(qū)的A5-E1的桶狀皮層功能上調(diào)更顯著(P0.05)。 結(jié)論：1.嗅覺損傷誘導(dǎo)的觸覺跨模式可塑性的研究中，桶狀皮層錐體神經(jīng)元的功能上調(diào)。2.嗅覺、觸覺存在的跨感覺可塑性和桶狀皮層神經(jīng)元功能上調(diào)在與長觸須代表區(qū)A5-E1表現(xiàn)更顯著。
[Abstract]:Aim: to investigate the cellular mechanism of the transmodal plasticity bucket cortex of tactile sensation after olfactory injury in mice, especially to compare the difference of the plasticity of pyramidal neurons between A5-E1 and C9-E4 in the tactile representative regions of the long and short tentacles. Methods 1. Behavioral experiment: mice were selected 12 days after birth to establish an animal model of increased tactile function induced by olfactory deprivation. The experiment was divided into two groups: normal group and deprivation group, 1 week to 2 weeks after olfactory deprivation. The mouse behavior was photographed by digital camera, and the number of times (frequency) of wobble of tentacles and the time of tentacles flexion were recorded in the video software in a unit time of 30 s. 2. Patch clamp electrophysiological experiment: 1 week after olfactory deprivation, isoflurane was inhaled to anesthetize the brain, and the brain barrel cortex was divided into C9-E4 region corresponding to short tentacles and A5-E1 region corresponding to long tentacles. Then the brain was separated and coronal sliced with 400 渭 m. The brain slices of C9-E4 area and A5-E1 area were placed in 25 C artificial cerebrospinal fluid in oxygenated artificial cerebrospinal fluid (ASCF), incubated for 1-2 hours, then transferred to the perfusion trough. The slices were perfused at 31C. The pyramidal neurons in the barrelled cortex were located with IR-DIC optical microscope and Nikon E600FN fluorescence microscope. The input and output curves of the pyramidal neurons in the barrelled cortex and the threshold potentials of the action potentials of the pyramidal neurons in the C9-E4 and A5-E1 regions of the brain were recorded by Axoclamp-200B amplifiers. The signal input Clampex10.2 software was used to obtain the inter-spike interval between the action potentials issued by the cluster, and the data were analyzed using Clampfit10.2 software. Results 1. The frequency of wobble of tentacles of olfactory deprived mice was higher than that of normal group, and the frequency of wobble of long tentacles in deprivation group was more significant than that of short tentacles. 2. Using patch clamp electrophysiological method, compared with normal group, In deprivation group, the function of pyramidal neurons in bucket cortex was enhanced (P 0.05), especially in the area of long tentacular representative area (A5-E1), the function of bucket cortex was upregulated more significantly (P 0.05). Conclusion 1. In the study of transmodal plasticity of tactile neurons induced by olfactory injury, the function of pyramidal neurons in bucket cortex is up-regulated. The transsensory plasticity of tactile and the up-regulation of the function of bucket cortical neurons were more significant in A5-E1 with long tentacles.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R338.1

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