本文選題：染色體　切入點：非整倍體　出處：《山西醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：研究流產(chǎn)絨毛染色體非整倍體及其雙親染色體著絲粒點(Cd)結(jié)構(gòu)變異和著絲粒蛋白(CENP)表達情況，探討子代非整倍體形成及其雙親染色體Cd結(jié)構(gòu)變異和CENP表達的關(guān)系。方法：以非整倍體流產(chǎn)絨毛及其雙親為研究組，以正常絨毛及其雙親為對照組。應(yīng)用染色體G顯帶技術(shù)、改良的著絲粒點-核仁組織區(qū)(Cd - NOR)同步銀染方法及免疫組化技術(shù)，對每例標(biāo)本進行染色體核型分析、Cd變異及CENP檢測。結(jié)果：①兩組流產(chǎn)絨毛染色體的Cd變異有顯著差異，研究組中的流產(chǎn)絨毛染色體Cd變異明顯高于對照組（t＝14.785，p㩳0.05）。②研究組中的流產(chǎn)絨毛染色體Cd變異高于雙親外周血染色體，差異有統(tǒng)計學(xué)意義,（t＝②14.965 p②㩳0.05，t＝③15.371，p③㩳0.05）。③對照組中的流產(chǎn)絨毛染色體Cd變異與其雙親外周血染色體比較，差異無統(tǒng)計學(xué)意義（t④＝2.463 p④㧐0.05，t＝⑤2.156 p⑤㧐0.05）④兩組流產(chǎn)絨毛染色體中均有CENP的表達，且研究組(灰度值：165.53±4.81)顯著低于對照組(灰度值：160.87±4.62)( t＝3.012，p㩳0.05)。⑤研究組非整倍體絨毛染色體中CENP含量低于其父方，差異有統(tǒng)計學(xué)意義（t①＝-4.279，p①㩳0.05），而與母方的差異無統(tǒng)計學(xué)意義（t②＝-1.927,p②㧐0.05)。⑥對照組正常絨毛染色體CENP含量低于其父方，差異有統(tǒng)計學(xué)意義（t③＝-3.008,p③㩳0.05),而與母方的差異無統(tǒng)計學(xué)意義(t④＝-1.234 p④㧐0.05)。⑦CENP的含量與Cd結(jié)構(gòu)變異呈負(fù)相關(guān)。 結(jié)論：Cd變異、CENP含量降低可能是染色體著絲粒不分離造成非整體形成的原因之一，且CENP的含量與其母親具有一定的同源性，染色體中CENP的含量與Cd變異呈負(fù)相關(guān)，即Cd變異數(shù)/細(xì)胞數(shù)越高，CENP的含量越少。
[Abstract]:Objective: to study the structural variation of chromosomal aneuploidy (CDD) and the expression of centromere protein (CENPP) in chromosomal aneuploidy of abortive villi and their parents. To investigate the relationship between the formation of aneuploidy and the relationship between CD structural variation and CENP expression in the chromosomes of their parents. Methods: using aneuploidy abortion villi and their parents as study group and normal villi and their parents as control group, chromosome G banding technique was used. The modified Cd-NORs simultaneous silver staining method and immunohistochemical technique were used to analyze the chromosome karyotype variation and CENP. Results there were significant differences between the two groups of abortive villi. The chromosome CD variation of abortive villi in the study group was significantly higher than that in the control group. The CD variation of abortive villi chromosomes in the study group was higher than that in the peripheral blood chromosomes of both parents, and the difference was statistically significant. 0.05t ~ (315.371n) p ~ (3)? The chromosome CD variation of abortive villi in the control group was not significantly different from that of their parents' peripheral blood chromosomes. 0. 05 TX 52.156 p5? The expression of CENP in the villus chromosomes of both groups was significantly lower in the study group (gray value: 165.53 鹵4.81) than that in the control group (gray value: 1: 160.87 鹵4.62). The CENP content in the chromosomes of aneuploidy villi in the study group was lower than that in its father's side, and the difference was statistically significant. There was no statistical difference between the two groups and the difference between them was not statistically significant (t _ (2)) -1.927 (P ~ (2))? The CENP content of normal villi in the control group was lower than that of its father's side, and the difference was statistically significant. 0. 05%, but there was no statistical difference between the two groups. There was a negative correlation between the content of 0. 05 and 7 CENP and the variation of CD structure. Conclusion the decrease in the content of CENs in the chromosome of 1: CD mutation may be one of the reasons for the formation of nonwhole in chromosome centromere dissection, and the content of CENP has some homology with its mother, and the content of CENP in chromosome is negatively correlated with the variation of CD. That is, the higher the number of CD variation / cell number, the less the content of CENP.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R394

【參考文獻】

相關(guān)期刊論文 前10條

1 梁前進,魯香鳳,程曉蕾,張煥相,何大澄,王永潮;乳腺癌中著絲粒蛋白CenpG的差異表達[J];高技術(shù)通訊;2002年01期

2 杜婧,墻克信,焦海燕;非整倍體患者及其雙親染色體著絲粒點(Cd)結(jié)構(gòu)和核仁組織區(qū)(NOR)rRNA基因活性研究[J];寧夏醫(yī)學(xué)雜志;2003年02期

3 唐寧;;染色體非整倍體畸變的產(chǎn)前診斷研究進展[J];實用醫(yī)技雜志;2008年09期

4 蔡燕;翁亞光;施瓊;徐遠(yuǎn)久;劉子杰;王應(yīng)雄;;著絲粒特異性蛋白CENP-Ⅰ的原核表達、純化及抗體制備[J];西南大學(xué)學(xué)報(自然科學(xué)版);2008年02期

5 焦海燕,張翠霞,陳銀濤,彭亮,于黎明,墻克信;不良孕產(chǎn)夫婦核仁組織區(qū)rRNA基因活性的研究[J];寧夏醫(yī)學(xué)院學(xué)報;2000年03期

6 王應(yīng)雄;翁亞光;何俊琳;劉學(xué)慶;鄭增淳;;正常人胚胎絨毛細(xì)胞染色體著絲粒點(Cd)變異的研究[J];遺傳;1995年02期

7 劉彥慧;黎麗芬;吳亞敏;王明波;石靜;袁冰;宋娟;何永明;魏代奎;;抗病毒治療對CMV感染者血染色體著絲粒點的影響[J];醫(yī)學(xué)研究雜志;2008年12期

8 劉彥軒;賈安奎;;流產(chǎn)孕婦HBV-DNA含量與染色體著絲粒點變異研究[J];中國婦幼保健;2010年19期

9 王應(yīng)雄;翁亞光;張湘蜀;鄭增淳;;高育齡婦女染色體著絲粒點研究[J];遺傳與疾病;1990年03期

10 王應(yīng)雄，，翁亞光，吳春英，何俊琳，鄭增淳;21三體患者及其雙親染色體著絲粒點研究[J];中華醫(yī)學(xué)遺傳學(xué)雜志;1994年04期

相關(guān)博士學(xué)位論文 前1條

1 蔡燕;著絲粒蛋白CENP-E和CENP-I與染色體分離關(guān)系研究[D];重慶醫(yī)科大學(xué);2008年

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