本文選題：肺動(dòng)脈高壓　切入點(diǎn)：高肺血流　出處：《廣州醫(yī)科大學(xué)學(xué)報(bào)》2016年05期 　論文類型：期刊論文

【摘要】：目的:簡化、改良建立大鼠高肺血流性肺動(dòng)脈高壓模型的方法。方法:采用腹主動(dòng)脈-下腔靜脈穿刺分流術(shù)建立大鼠高肺血流性肺動(dòng)脈高壓模型,并以組織黏合劑閉合腹主動(dòng)脈左側(cè)穿刺口。分流11周后,觀察下腔靜脈變化情況,通過右心導(dǎo)管術(shù)檢測大鼠肺動(dòng)脈壓力,以血?dú)夥治龇椒y定動(dòng)靜脈血氧飽和度,分離右心室與左心室(包含室間隔)并計(jì)算右心室肥厚指數(shù)。結(jié)果:腹主動(dòng)脈阻斷時(shí)間少于2 min;11周后分流組大鼠下腔靜脈明顯增粗并存在震顫及動(dòng)靜脈血混合,其分流瘺口之上的下腔靜脈血氧飽和度約等于腹主動(dòng)脈血氧飽和度,但明顯高于假手術(shù)組下腔靜脈血氧飽和度(P0.01),分流組大鼠平均肺動(dòng)脈壓及右心室肥厚指數(shù)顯著高于假手術(shù)組(P0.01)。結(jié)論:采用組織黏合劑的方法能簡易、快速地完成腹主動(dòng)脈-下腔靜脈穿刺分流術(shù),有效地建立大鼠高肺血流性肺動(dòng)脈高壓模型。
[Abstract]:Objective: to simplify and improve the method of establishing a rat model of pulmonary hypertension with high pulmonary blood flow. Methods: a rat model of pulmonary hypertension with high pulmonary blood flow was established by abdominal aorta-inferior vena cava shunt. After 11 weeks of shunt, the changes of inferior vena cava were observed, pulmonary artery pressure was measured by right cardiac catheterization, and arterial and venous oxygen saturation was measured by blood gas analysis. The right ventricle and left ventricle (including septum) were separated and the right ventricular hypertrophy index was calculated. Results: the abdominal aorta occlusion time was less than 2 mins and 11 weeks later, the inferior vena cava was thickened and there was tremor and mixed arteriovenous blood in the shunt group. The oxygen saturation of the inferior vena cava above the shunt fistula is about equal to that of the abdominal aorta. The mean pulmonary artery pressure and the right ventricular hypertrophy index in shunt group were significantly higher than those in sham operation group. Conclusion: the method of tissue adhesive can be used as a simple method. Abdominal aorta-inferior vena cava shunt (IVC) was performed quickly to establish a rat model of pulmonary hypertension with high pulmonary blood flow.
【作者單位】： 廣州醫(yī)科大學(xué)附屬廣州市婦女兒童醫(yī)療中心心臟中心;
【基金】：國家自然科學(xué)基金(81100233)
【分類號(hào)】：R544.1;R-332

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